Production of Xylanase of Bacillus coagulans and its bleaching potential

Choudhury, Bijan; Chauhan, Shakti; Singh, Suren; Ghosh, Purnendu

doi:10.1007/s11274-005-9033-0

Cited by 29 publications

(9 citation statements)

References 21 publications

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“…GthC5ProXyl displayed better and longer effects on the hot and alkaline pulp samples than the wild type xylanase ( Table 2). Similar effects of xylanases from B. licheniformis [41] B. pumilus [42] and B. coagulans [43] have been reported. After tagging the proline at C terminal, its alkalistability and thermostability was enhanced thus making the proline tagged xylanase as a biocatalyst choice for bio-bleaching in paper pulps.…”

Section: Discussionsupporting

confidence: 74%

C-Terminal proline-rich sequence broadens the optimal temperature and pH ranges of recombinant xylanase from Geobacillus thermodenitrificans C5

Irfan

Güler

Özer

et al. 2016

Enzyme and Microbial Technology

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Section: Discussionsupporting

confidence: 74%

C-Terminal proline-rich sequence broadens the optimal temperature and pH ranges of recombinant xylanase from Geobacillus thermodenitrificans C5

Irfan

Güler

Özer

et al. 2016

Enzyme and Microbial Technology

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“…Compared to the levels of xylanase produced by thermotolerant bacteria and fungi (Table 7), strain TISTR1948 also showed higher xylanase activity than Bacillus coagulans, 30) which gave only 2,420 U/g of birch-wood xylan). Su et al 32) reported very high xylanase activity, of 3,078 U/mL or 79,535 U/g of corn cob, by the fungus Thermomyces lanuginosus SDYKY-1 under optimal conditions obtained by a statistically based experimental design (Plackett-Burman and CCD).…”

Section: Y(xylanase Activity)mentioning

confidence: 92%

Optimization of Cellulase-Free Xylanase Production by Thermophilic Streptomyces thermovulgaris TISTR1948 through Plackett-Burman and Response Surface Methodological Approaches

Chaiyaso

Kuntiya

Techapun

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Cellulase-free xylanase production by thermophilic Streptomyces thermovulgaris TISTR1948 was cultivated in a basal medium with rice straw as sole source of carbon and as an inducible substrate. Variable medium components were selected in accordance with the Plackett-Burman experimental design. The optimization conditions of physical factors (pH and temperature levels) were then combined in further studies through the response surface methodology approach. Only two significant components, rice straw and yeast extract, were chosen for the optimization studies. A second-order quadratic model was constructed by central composite design (CCD). The model revealed that both pH and temperature levels were significant, and were dependent on xylanase production. Under these experimental designs, the xylanase yield increased from 51.11 to 274.49 U/mL (3,400 to 10,000 U/g of rice straw) or about 537% higher than an unoptimized basal medium. The optimum conditions to achieve maximum yield of xylanase were 27.45 g/L of rice straw and 5.42 g/L of yeast extract under relatively neutral conditions of pH 7.11, 50.03 °C, and a incubation period.

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“…Continuing interest in Bacillus sp. xylanases is also evident in the recent papers, among others, handling several aspects from enzyme production to application (Avcioglu et al 2005;Heck et al 2005;Sapre et al 2005;Virupakshi et al 2005;Battan et al 2006;Choudhury et al 2006;etc.). The present paper reports the exceptionally high production, purification, characterization and application in Kraft pulp biobleaching of an extracellular xylanase produced by Bacillus sp.…”

Section: Introductionmentioning

confidence: 88%

Production, purification and characterization of Bacillus sp. GRE7 xylanase and its application in eucalyptus Kraft pulp biobleaching

Kiddinamoorthy

Anceno

Haki

et al. 2007

World J Microbiol Biotechnol

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Production of extracellular xylanase from Bacillus sp. GRE7 using a bench-top bioreactor and solidstate fermentation (SSF) was attempted. SSF using wheat bran as substrate and submerged cultivation using oat-spelt xylan as substrate resulted in an enzyme productivity of 3,950 IU g À1 bran and 180 IU ml À1 , respectively. The purified enzyme had an apparent molecular weight of 42 kDa and showed optimum activity at 70°C and pH 7. The enzyme was stable at 60-80°C at pH 7 and pH 5-11 at 37°C. Metal ions Mn 2+ and Co 2+ increased activity by twofold, while Cu 2+ and Fe 2+ reduced activity by fivefold as compared to the control. At 60°C and pH 6, the K m for oat-spelt xylan was 2.23 mg ml À1 and V max was 296.8 IU mg À1 protein. In the enzymatic prebleaching of eucalyptus Kraft pulp, the release of chromophores, formation of reducing sugars and brightness was higher while the Kappa number was lower than the control with increased enzyme dosage at 30% reduction of the original chlorine dioxide usage. The thermostability, alkali-tolerance, negligible presence of cellulolytic activity, ability to improve brightness and capacity to reduce chlorine dioxide usage demonstrates the high potential of the enzyme for application in the biobleaching of Kraft pulp.

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Production of Xylanase of Bacillus coagulans and its bleaching potential

Cited by 29 publications

References 21 publications

C-Terminal proline-rich sequence broadens the optimal temperature and pH ranges of recombinant xylanase from Geobacillus thermodenitrificans C5

C-Terminal proline-rich sequence broadens the optimal temperature and pH ranges of recombinant xylanase from Geobacillus thermodenitrificans C5

Optimization of Cellulase-Free Xylanase Production by Thermophilic Streptomyces thermovulgaris TISTR1948 through Plackett-Burman and Response Surface Methodological Approaches

Production, purification and characterization of Bacillus sp. GRE7 xylanase and its application in eucalyptus Kraft pulp biobleaching

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