Optimization of Cellulase-Free Xylanase Production by Thermophilic Streptomyces thermovulgaris TISTR1948 through Plackett-Burman and Response Surface Methodological Approaches

Abstract

Cellulase-free xylanase production by thermophilic Streptomyces thermovulgaris TISTR1948 was cultivated in a basal medium with rice straw as sole source of carbon and as an inducible substrate. Variable medium components were selected in accordance with the Plackett-Burman experimental design. The optimization conditions of physical factors (pH and temperature levels) were then combined in further studies through the response surface methodology approach. Only two significant components, rice straw and yeast extract, were chosen for the optimization studies. A second-order quadratic model was constructed by central composite design (CCD). The model revealed that both pH and temperature levels were significant, and were dependent on xylanase production. Under these experimental designs, the xylanase yield increased from 51.11 to 274.49 U/mL (3,400 to 10,000 U/g of rice straw) or about 537% higher than an unoptimized basal medium. The optimum conditions to achieve maximum yield of xylanase were 27.45 g/L of rice straw and 5.42 g/L of yeast extract under relatively neutral conditions of pH 7.11, 50.03 °C, and a incubation period.

Diversity and Antimicrobial Activity of Actinomycetes from Fecal Sample of Rhinoceros Beetle Larvae

“…2) (Rivas et al, 2003). Clone C07, C08, C20와 99.6%의 상동성을 보인 Streptomyces thermovulgaris의 경우 호열성 균주 TISTR1948으로부터 xylanase 를 분리하여 산업적으로 이용하기 위한 연구가 수행되었다 (Chaiyaso et al, 2011). Clone C19, C23과 높은 상동성을 보인 Xylanimicrobium pachnoda VPCX2 T 는 풍뎅이(Pachnoda marginata) 유충의 후장에서 분리되었고 xylanase와 endoglucanase 활성을 가지고 있다 (Stackebrandt and Schumann, 2004 선발된 27균주의 16S rRNA 유전자 염기서열을 EzBioCloud 데이터베이스에서 검색된 유사 균주들과 계통수를 작성하여 계 통학적 다양성을 분석하였다 (Fig.…”

Secretion of recombinant thermo-alkali-stable endoxylanase of polyextremophilic Bacillus halodurans TSEV1 and its utility in generating xylooligosaccharides from renewable agro-residues

“…The statistical optimization of the selected variables using CCD design of RSM (Table 1) led to a 4-fold higher extracellular enzyme titre than that in the unoptimized medium and conditions. Although there are reports of statistical optimization of xylanase production by the wild bacterial strains [18,32], there is no such attempt for optimizing recombinant xylanase production by recombinant E. coli. The statistical optimization is known to provide interactive optimal conditions among various independent variables.…”

Production and Characterization of Partially Purified Thermostable Endoxylanase and Endoglucanase from Novel Actinomadura geliboluensis and the Biotechnological Applications in the Saccharification of Lignocellulosic Biomass

“…Similarly, Streptomyces thermovulgaris TISTR1948 (Chaiyaso et al 2011) and Streptomyces sp. B-PNG23 (Bettache et al 2013) secreted high amounts of enzyme when the yeast extract was used in the medium.…”