Production of xylan degrading endo-1, 4-β-xylanase from thermophilic Geobacillus stearothermophilus KIBGE-IB29

Bibi, Zainab; Ansari, Asma; Zohra, Raheela Rahmat; Aman, Afsheen; Qader, Shah Ali Ul

doi:10.1016/j.jrras.2014.08.001

Cited by 58 publications

(40 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar finding to this study was made by other authors who reported 30°C as optimal temperature for xylanase and cellulase production by different Bacillus species (Nagar et al, 2012;Bai et al, 2012;; and very close to the finding of Mukesh who reported that 32°C was optimal for cellulase production by B. cereus MRK1. Contrary to the result of this study however, maximum production of xylanase and cellulase by other bacterial species at both lower and higher incubation temperatures have been reported (Sepahy et al, 2011;Bibi et al, 2014), which is suggestive that optimal incubation temperature for cellulase and xylanase production is dependent on bacterial strain (Gautam et al, 2011).…”

Section: Effect Of Incubation Temperaturecontrasting

confidence: 99%

Optimization of Process Conditions for the Production of Holocellulase by a <i>Bacillus</i> Species Isolated from Nahoon Beach Sediments

Fatokun¹,

Nwodo²,

Olaniran³

et al. 2017

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

Production of holocellulase by a newly isolated marine Bacillus species via submerged fermentation technique at varying cultivation conditions was investigated. Enzyme production was optimized by altering one variable per time. Moreover effect of lignocellulosic saccharides in inducing cellulase and xylanase production was also investigated. The 16S ribosomal Deoxyribonucleic Acid (16S rDNA) gene sequence analysis exhibited 98% sequence similarity of the isolate with other Bacillus species in the gene bank and was deposited with the accession number KX524510. Optimal xylanase and cellulase production was attained at pH 7, temperature 30°C and agitation speed of 50 and 150 rpm. Furthermore, maximum xylanase and cellulase production were both achieved at 60 h corresponding to the late stationary growth stage, with activity of 16.6 and 0.061 U mL −1 respectively. Xylanase production was maximally induced by beechwood xylan, xylose and arabinose with activities of 13.59, 8.78 and 1.90 (U mL −1 ) respectively; while cellulase production was induced by carboxymethyl cellulose only and no cellulase activity was detected in the culture supernatant of the other carbon sources tested. Optimization increased cellulase and xylanase yields being 0.006-0.061 and 0.23-16.6 (U mL −1 ) from unoptimized to optimized respectively. The results of the study suggest the bacterial strain to be a proficient producer of cellulase and xylanase with potentials in biotechnological application.

show abstract

Section: Effect Of Incubation Temperaturecontrasting

confidence: 99%

Optimization of Process Conditions for the Production of Holocellulase by a <i>Bacillus</i> Species Isolated from Nahoon Beach Sediments

Fatokun¹,

Nwodo²,

Olaniran³

et al. 2017

American Journal of Biochemistry and Biotechnology

View full text Add to dashboard Cite

show abstract

“…A thermophilic bacterium namely G. stearothermophilus KIBGE-IB29 was previously isolated from the soil collected around the blast furnace site of Pakistan Steel Mill, Karachi, Pakistan (Bibi et al 2014). Bacterium was inoculated into optimized fermentation medium containing (gL −1 ) corncob xylan, 5.0; peptone, 5.0; yeast extract, 2.0; meat extract, 1.0; K 2 HPO 4 , 2.5; KH 2 PO 4 , 0.5; (NH 4 ) 2 SO 4 , 0.5; and CaCl 2 , 0.1 with pH-6.0 and incubated at 60 °C for 24 h. Cells were harvested by centrifugation at 40248×g for 15.0 min at 4 °C.…”

Section: Production Of Endo-β-14-xylanase From Geobacillus Stearothementioning

confidence: 99%

Calcium alginate matrix increases the stability and recycling capability of immobilized endo-β-1,4-xylanase from Geobacillus stearothermophilus KIBGE-IB29

2015

Self Cite

View full text Add to dashboard Cite

Exploration of microbial pool from extremely diversified ecosystem is significantly important for various industrial applications. Bacterial communities from extreme habitats including volcanic vents, hot springs, and industrial sectors are eagerly explored for the isolation of thermophiles. Geobacillus stearothermophilus KIBGE-IB29, isolated from blast furnace site of a steel processing industry, is capable of producing thermostable endo-β-1,4-xylanase. In the current study, this enzyme was immobilized within calcium alginate beads using entrapment technique. Amalgamation of sodium alginate (40.0 gL(-1)) and calcium chloride (0.4 M) was used for the formation of immobilized beads. It was observed that temperature (50 °C) and pH (7.0) optima of immobilized enzyme remained same, but enzyme-substrate reaction time increased from 5.0 to 30.0 min as compared to free enzyme. Diffusion limit of high molecular weight xylan (corncob) caused a decline in V max of immobilized enzyme from 4773 to 203.7 U min(-1), whereas K m value increased from 0.5074 to 0.5722 mg ml(-1) with reference to free enzyme. Immobilized endo-β-1,4-xylanase showed its stability even at high temperatures as compared to free enzyme and retained 18 and 9 % residual activity at 70 and 80 °C, respectively. Immobilized enzyme also exhibited sufficient recycling efficiency up to five reaction cycles which indicated that this enzyme can be a plausible candidate in paper and pulp industry.

show abstract

“…However, different optimum temperature has been observed in various thermophilic isolates namely G. stearothermophilus and B. halodurans [35,36]. Xylanase from bacterial sources are active and stable in a wide range of pH and temperature conditions.…”

Section: Discussionmentioning

confidence: 99%

Production of Industrially Important Enzymes by Thermobacilli Isolated From Hot Springs of India

Dhyani

Gururani

Selim

et al. 2017

RIB

View full text Add to dashboard Cite

Enzymes from thermophilic bacteria have received great attention for their potential applications in various industrial sectors. The present study deals with the production of five thermozymes (amylase, lipase, xylanase, protease and cellulase) from 10 thermophilic bacterial species, originally isolated from two hot springs namely Soldhar and Ringigad in Uttarakhand Himalaya, India. The bacterial isolate GBPI_25 produced maximum amylase (1217.86 U/ml) at 45 °C and 5 pH, GBPI 3 produced maximum lipase (22.59 U/ml) at 65 °C and 9 pH, GBPI_25 produced maximum xylanase (98.07 U/ml) at45 °C and 9 pH, GBPI_35 produced maximum protease (16.66 U/ml) at 55 °C and 9 pH, and GBPI 4 produced maximum cellulose (108.68 U/ml) at 45 °C and 5 pH. Crude enzyme preparations showed thermal and pH activities at broad temperature and pH range between 10-100 °C and 3-11 pH, respectively, with different temperature and pH optima. Amylase, xylanase and cellulase showed maximum activity at 50 °C while lipase and protease showed higher activity at 40 and 60 °C, respectively. Enzyme activity at wide temperature range-cellulase and protease from 10-100 °C, amylase and xylanasefrom10-90 °C, and lipase activity from 10-80 °C were the remarkable records from this study. Similarly, pH range for amylase and lipase activity was recorded from 4-11, for xylanase from 3-9, and for protease and cellulase from 3-10. All the thermozymes showed maximum stability at 40 °C and pH 5 except cellulase that showed higher stability at40 °C and neutral pH.

show abstract

Production of xylan degrading endo-1, 4-β-xylanase from thermophilic Geobacillus stearothermophilus KIBGE-IB29

Cited by 58 publications

References 30 publications

Optimization of Process Conditions for the Production of Holocellulase by a <i>Bacillus</i> Species Isolated from Nahoon Beach Sediments

Optimization of Process Conditions for the Production of Holocellulase by a <i>Bacillus</i> Species Isolated from Nahoon Beach Sediments

Calcium alginate matrix increases the stability and recycling capability of immobilized endo-β-1,4-xylanase from Geobacillus stearothermophilus KIBGE-IB29

Production of Industrially Important Enzymes by Thermobacilli Isolated From Hot Springs of India

Contact Info

Product

Resources

About