Optimization of Process Conditions for the Production of Holocellulase by a <i>Bacillus</i> Species Isolated from Nahoon Beach Sediments

Abstract

Production of holocellulase by a newly isolated marine Bacillus species via submerged fermentation technique at varying cultivation conditions was investigated. Enzyme production was optimized by altering one variable per time. Moreover effect of lignocellulosic saccharides in inducing cellulase and xylanase production was also investigated. The 16S ribosomal Deoxyribonucleic Acid (16S rDNA) gene sequence analysis exhibited 98% sequence similarity of the isolate with other Bacillus species in the gene bank and was deposited with the accession number KX524510. Optimal xylanase and cellulase production was attained at pH 7, temperature 30°C and agitation speed of 50 and 150 rpm. Furthermore, maximum xylanase and cellulase production were both achieved at 60 h corresponding to the late stationary growth stage, with activity of 16.6 and 0.061 U mL −1 respectively. Xylanase production was maximally induced by beechwood xylan, xylose and arabinose with activities of 13.59, 8.78 and 1.90 (U mL −1 ) respectively; while cellulase production was induced by carboxymethyl cellulose only and no cellulase activity was detected in the culture supernatant of the other carbon sources tested. Optimization increased cellulase and xylanase yields being 0.006-0.061 and 0.23-16.6 (U mL −1 ) from unoptimized to optimized respectively. The results of the study suggest the bacterial strain to be a proficient producer of cellulase and xylanase with potentials in biotechnological application.

Studies on peroxidase production and detection of Sporotrichum thermophile-like catalase-peroxidase gene in a Bacillus species isolated from Hogsback forest reserve, South Africa

“…This indicates that production of peroxidase by the bacteria was growth associated. The decrease observed in the level of peroxidase produced by the bacteria after the optimal period of incubation may be as a result of denaturation or proteolysis (Fatokun et al., 2017). This finding contradicts a number of similar studies where the maximum peroxidase was produced at 72 h of incubation (Nour El-Dein et al., 2014; Falade et al., 2019b).…”

Statistical optimization of cellulase production by halophilic strains isolated from Tunisian Sebkhas using the renewable marine biomass waste, Posidonia oceanica, as a cellulosic substrate

“…The levels of factors were selected by taking into account the operating experimental limits, and the previous studies. [34][35][36] The domain and matrix characteristics of Plackett-Burman experimental design used for the optimization of cellulase production were presented in Tables 1&2, respectively. As shown in Table 2, the experimental and predicted values of CMCase activity match quite well.…”

Screening the Effect of the Expression Medium and Growth Conditions on the Performance of Engineered Xylanase Produced by Immobilized Recombinant E. Coli

“…Thus, any alteration of cultivation temperature above or below the optimum temperature could lead to alteration in xylanase protein structure and change the three-dimensional shape or xylanase denaturation consequently altering its catalytic activities or its ability to bind substrates [30]. Resulting change in the reaction and lead to reduction in cellular metabolic activities rate, with subsequent inhibition in protein synthesis and cell growth [31].…”