Production of tropane alkaloids by small-scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots

Enhanced production of tropane alkaloids in transgenic Scopolia parviflora hairy root cultures over-expressing putrescine N-methyl transferase (PMT) and hyoscyamine-6β-hydroxylase (H6H)

“…Thus, PMT and H6H enzymes are important targets for metabolic engineering in order to improve the yields of scopolamine. Also, as roots are the main sites of alkaloid biosynthesis in Scopolia parviflora, the generation of hairy roots followed by their mass culture would provide increased TA productions (Min et al 2007a;Palazon et al 2008).…”

Increased puerarin biosynthesis during in vitro shoot formation in Pueraria tuberosa grown in growtek bioreactor with aeration

“…Similar to M. citrifolia (Ahmed et al 2008), higher biomass was also obtained in Scopolia parviflora with high aeration volume, and alkaloid content increased with low aeration (Min et al 2007). Aeration is not always beneficial for high secondary metabolites accumulation but sometimes it depends on the condition of culture stage and selected explants source (Ahmed et al 2008;Senoussi et al 2009).…”

Increasing Scopolamine content in Hairy Roots of Atropa belladonna using Bioreactor

“…Notwithstanding, a high volume doesn"t always enhance the accumulation of compounds in plant cultures. Min et al (2007) reported higher biomass production from Scopolia parviflora when cultured in a high aeration volume (0.4 vvm), but increased alkaloid contents at a lower volume (0.1 wm). These results suggested that the aeration volume should be adjusted based on the selected ex-plant source and the cultural stage for biomass production.…”

“…The present study indicated that hairy roots of A. belladonna required high concentration of aeration compared to other root cultures and plant cells. Scopolamine production is dependent on aeration such that the oxygen molecules activate one of the important key enzymes (6b-hydroxyhyoscyamine) in scopolamine production in plant cells and tissue cultures (Hashimoto et al 1989;Min et al 2007;Kardilo et al 2010). Improvements in H6H activity causes more hyosiamine to change into scopolamine (Palazon et al 2003).…”