Increasing Scopolamine content in Hairy Roots of Atropa belladonna using Bioreactor

Habibi, Peyman; Piri, Khosro; Deljo, Ali; Moghadam, Y Ahmadi; Ghiasvand, Taiebeh

doi:10.1590/s1516-8913201400276

Cited by 19 publications

(5 citation statements)

References 38 publications

(40 reference statements)

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“…Additionally, by manipulating metabolic pathways (introducing new genes or silencing selected ones), it is possible to create efficient plant-based production systems. Plant cells or tissue cultures obtained in this way can be transferred to bioreactors, allowing them to be cultivated on a larger scale, which can directly lead to a further increase in productivity and even their use on an industrial scale [ 33 , 34 ]. The entire set of factors described above makes it possible to develop highly efficient plant culture systems, the metabolites of which can be used in the pharmaceutical and medical industries [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Methyl Jasmonate Effect on Betulinic Acid Content and Biological Properties of Extract from Senna obtusifolia Transgenic Hairy Roots

et al. 2021

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It is known that Senna obtusifolia has been used in medicine since ancient times due to the content of many valuable compounds with a pro-health effect. One of them is betulinic acid, which is a pentacyclic triterpene with antimalarial, antiviral, anti-inflammatory and anticancer properties. In this work, a continuation of our previous research, an attempt was made to increase the level of betulinic acid accumulation by the cultivation of transgenic hairy roots that overexpress the squalene synthase gene in a 10 L sprinkle bioreactor with methyl jasmonate elicitation. We present that the applied strategy allowed us to increase the content of betulinic acid in hairy root cultures to the level of 48 mg/g dry weight. The obtained plant extracts showed a stronger cytotoxic effect on the U87MG glioblastoma cell line than the roots grown without elicitors. Additionally, the induction of apoptosis, reduction of mitochondrial membrane potential, chromosomal DNA fragmentation and activation of caspase cascades are demonstrated. Moreover, the tested extract showed inhibition of topoisomerase I activity.

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Section: Discussionmentioning

confidence: 99%

Methyl Jasmonate Effect on Betulinic Acid Content and Biological Properties of Extract from Senna obtusifolia Transgenic Hairy Roots

et al. 2021

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“…However, Habibi et al [ 56 ] reported that apart from aeration, media, and culture agitation can also be important factors, which enhanced cell growth and increased scopolamine production of Atropa belladonna hairy roots in bioreactor. BCB cultivation involves mechanical stress caused by inflows of air and high agitation, which often results in some slight damage of hairy roots that manifests as foaming of growth medium.…”

Section: Discussionmentioning

confidence: 99%

Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Vinterhalter

Banjac

Vinterhalter

et al. 2021

Plants

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The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.

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“…belladonna Hairy root cultures were produced by inoculating sterile leaf explants with Agrobacterium rhizogenes strain 15834 (Habibi et al 2015) (Fig. 1a).…”

Section: Methodsmentioning

confidence: 99%

Removal of Phenol byA. belladonna L.Hairy Root

Mazaheri

Piri

2015

International Journal of Phytoremediation

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Phenolic compounds that present in the several industries are harmful and dangerous for human health. In this study we have studied the potential of Atropa belladonna hairy roots in phenol removal of wastewater. The optimal conditions for the removal process were evaluated using different phenol (10-500 mg.1(-1)) and H2O2 (1-15 Mm) concentrations. In the presence of H2O2, Roots were able to remove phenol concentrations up to 500 mg.1(-1). in the wide range of pH (4-9), reaching high removal efficiency. When roots were re-used for five consecutive cycles, phenol removal efficiency decreased from 98-62%, in the last cycle. After the removal process, the solutions were obtained from the experiment were estimated for their toxicity using a test with Lactaca sativa L. seeds. Results showed that the treated solution was less toxic than the parent solution.

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Increasing Scopolamine content in Hairy Roots of Atropa belladonna using Bioreactor

Abstract: ABSTRACT

Cited by 19 publications

References 38 publications

Methyl Jasmonate Effect on Betulinic Acid Content and Biological Properties of Extract from Senna obtusifolia Transgenic Hairy Roots

Methyl Jasmonate Effect on Betulinic Acid Content and Biological Properties of Extract from Senna obtusifolia Transgenic Hairy Roots

Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Removal of Phenol byA. belladonna L.Hairy Root

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