Increased puerarin biosynthesis during in vitro shoot formation in Pueraria tuberosa grown in growtek bioreactor with aeration

Sharma, Varsha; Goyal, Shaily; Ramawat, K. G.

doi:10.1007/s12298-011-0049-7

Cited by 24 publications

(7 citation statements)

References 21 publications

(23 reference statements)

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“…Growtek bioreactor (1 L, Tarson, India) was used with 10 % v/v aeration provided by inserting a needle through septum in the side tube. The details of Growtek bioreactor's design have been described earlier (Sharma et al 2011). The explants used for the experiment were single nodal pieces of 0.5 cm obtained from 6 weeks old tissuecultured plants in all the experiments.…”

Section: Methodsmentioning

confidence: 99%

Shoot culture of Bacopa monnieri: standardization of explant, vessels and bioreactor for growth and antioxidant capacity

Jain

Sharma

Ramawat

2012

Physiol Mol Biol Plants

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Standardization of biomass production in different vessels and bioreactor using explants and media for growth, total phenolic content and antioxidant capacity of shoot culture of Bacopa monnieri is described. Maximum number of shoots per explant, higher explants response irrespective of the type of explants, and higher shoot length was obtained on MS medium containing BAP (2.5 mg l −1 ) and IAA (0.01 mg l −1 ) with 3 % sucrose. This medium was selected by varying BAP concentration and recorded optimal for shoot culture on gelled medium. The condition of 0.5 cm explant size and 20 explant/40 ml (1 explant/2 ml) was optimal for high explant response, number of shoots per explant regenerated and shoots length. Among the different vessels used, maximum growth index was achieved in Growtek bioreactor (10.0) followed by magenta box (9.16), industrial glass jar (7.7) and conical flask (7.2). The cultures grown in conical flask (100 ml) were used as control. The total phenolic content and antioxidant capacity of in vitro grown plants was higher to that recorded for in vivo material. Among in vitro regenerated plants, the activity was maximal in the tissues grown in 250 ml conical flask. The most critical function for vessels is to support the optimum profusion (growing area for maximum growth) of shoots and for B. monnieri, Growtek bioreactor supported 1980 shoots l −1 medium as compared to control (938). Growtek bioreactor was considered effective system to produce B. monnieri biomass in culture without loss of antioxidant properties.

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Section: Methodsmentioning

confidence: 99%

Shoot culture of Bacopa monnieri: standardization of explant, vessels and bioreactor for growth and antioxidant capacity

Jain

Sharma

Ramawat

2012

Physiol Mol Biol Plants

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“…The science of biotechnology has provided us the required tools and techniques for mass cloning, improvement in medicinal plants and increased production of useful metabolites (Arora et al 2010 ;Sharma et al 2011a ). Conventional methods of propagation have their own shortcomings with respect to commercial level production and quality control.…”

Section: Why Biotechnology Of Lianas?mentioning

confidence: 99%

“…The propagation in its natural habitat is becoming a hard phenomenon. Therefore, biotechnology helps in conservation of such plants and meets the growing requirements (Arora et al 2010 ;Sharma et al 2011a ). The lianas selected for detailed study in this article are traditionally used in Indian system of medicine and are well-established medicinal plants.…”

Section: Introductionmentioning

confidence: 99%

Biodiversity of Lianas

Parthasarathy¹

2015

Sustainable Development and Biodiversity

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This book series provides complete, comprehensive and broad subject based reviews about existing biodiversity of different habitats and conservation strategies in the framework of different technologies, ecosystem diversity, and genetic diversity. The ways by which these resources are used with sustainable management and replenishment are also dealt with. The topics of interest include but are not restricted only to sustainable development of various ecosystems and conservation of hotspots, traditional methods and role of local people, threatened and endangered species, global climate change and effect on biodiversity, invasive species, impact of various activities on biodiversity, biodiversity conservation in sustaining livelihoods and reducing poverty, and technologies available and required. The books in this series will be useful to botanists, environmentalists, marine biologists, policy makers, conservationists, and NGOs working for environment protection.More information about this series at

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“…The calculated productivities of IM and IG, which are much less abundant than mangiferin (and consequently, more expensive), may be interesting from a practical perspective. The obtained results indicate that C. genistoides shoot cultures could be successfully maintained in commercially available and easily multipliable temporary-immersion (RITA) (Zobayed et al 2004) or raft (Growtek) bioreactor systems (Sharma et al 2011), and utilized for the production of biologically active xanthone and benzophenone derivatives. Fig.…”

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confidence: 95%

Xanthone, benzophenone and bioflavonoid accumulation in Cyclopia genistoides (L.) Vent. (honeybush) shoot cultures grown on membrane rafts and in a temporary immersion system

Kokotkiewicz

Buciński

Łuczkiewicz

2014

Plant Cell Tiss Organ Cult

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In vitro shoots of the commercially important South-African legume Cyclopia genistoides were grown in different liquid culture systems [membrane rafts (MR) and a temporary immersion bioreactor (TIB)] and evaluated for the accumulation of phenolic secondary metabolites. The major constituents of the investigated cultures were medicinally relevant xanthones [mangiferin (M) and isomangiferin (IM)] and benzophenone derivatives [iriflophenone 3-C-b-glucoside (IG)]. The highest concentrations of M, IM and IG in MR-grown shoots were 1,843.59, 712.02 and 594.29 mg 100 g -1 dry wt, respectively. Bioreactor cultivation provided higher peak concentrations of M (2,622.70 mg 100 g -1 dry wt), IM (757.40 mg 100 g -1 dry wt) and IG (648.30 mg 100 g -1 dry wt) which corresponded to the respective productivities of 5.48, 1.58 and 3.04 mg l -1 d -1 . The results indicate that TIB cultures of C. genistoides may be utilized as an alternative source of the above constituents, particularly IM and IG, which are relatively expensive and so far hardly available from commercial sources.

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Increased puerarin biosynthesis during in vitro shoot formation in Pueraria tuberosa grown in growtek bioreactor with aeration

Cited by 24 publications

References 21 publications

Shoot culture of Bacopa monnieri: standardization of explant, vessels and bioreactor for growth and antioxidant capacity

Shoot culture of Bacopa monnieri: standardization of explant, vessels and bioreactor for growth and antioxidant capacity

Biodiversity of Lianas

Xanthone, benzophenone and bioflavonoid accumulation in Cyclopia genistoides (L.) Vent. (honeybush) shoot cultures grown on membrane rafts and in a temporary immersion system

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