Production of trans-free interesterified fat using indigenously immobilized lipase

Kavadia, Monali R.; Yadav, Manish G.; Vadgama, Rajeshkumar N.; Odaneth, Annamma A.; Lali, Arvind

doi:10.1080/10826068.2019.1566142

Cited by 4 publications

(1 citation statement)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to temperature tolerance, pH stability, organic solvent compatibility, reusability, substrate specificity, and enantioselective hydrolysis, lipases have gained a predominance group of protein-based biocatalysts . These enzymes are currently used in various industrial fields such as in alternative energy production (biodiesel), surfactants production, racemic resolution of compounds, fine chemicals (ester synthesis) production, life-cycle assessment through utilization of wastewater, etc. − Lipases are efficiently accessible from a low-cost and renewable raw materials and could be isolated from plants or secreted by fungi, bacteria, or yeasts due to their metabolic activity. , Nowadays, lipases are commercially available as a solution or immobilized form, with an aspect of new immobilization eco-friendly techniques based on the application of the natural polymers …”

Section: Introductionmentioning

confidence: 99%

A New Spectrophotometric Assay for Measuring the Hydrolytic Activity of Lipase from Thermomyces lanuginosus: A Kinetic Modeling

Šibalić

Šalić

Zelić

et al. 2020

ACS Sustainable Chem. Eng.

View full text Add to dashboard Cite

In the present study, several key reaction parameters that affect the hydrolytic rate of the p-NP palmitate by Thermomyces lanuginosus lipase were studied to define new spectrophotometric assay for measuring the lipase activity. The influence of the substrate and enzyme concentration, reaction time, pH, organic solvents (acetonitrile, methanol, isopropanol, ethanol, 2-ethoxyethanol, DMSO, acetone, and 1,4-dioxane) and emulsifier agents (Gum Arabic, PEG 4000, PEG 6000, Triton X-45, Triton X-100, SDS, CTAB, Tween 20, Tween 40, Tween 80, and Span 80) on the reaction rate was analyzed. Two kinetic models, Michaelis–Menten kinetic model and Hill kinetic model were suggested. The results obtained by developed models were compared, and it was shown that Hill kinetic model describes experimental data better. A spectrophotometric test is designed for its primary applications for the measuring of lipase activity during the enzymatic biodiesel production process and for the measurement of lipase activity during its production.

show abstract