Production of thermostable multiple enzymes from <i>Bacillus amyloliquefaciens</i> KUB29

Devaraj, Kubendran; Aathika, Salma; Periyasamy, Karthik; Periyaraman, Premkumar Manickam; Palaniyandi, Shanmugam; Sivanesan, Subramanian

doi:10.1080/14786419.2018.1425857

Cited by 13 publications

(9 citation statements)

References 155 publications

(161 reference statements)

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“…AM1 ( Table 1 ) revealed similar results to those described for Bacillus amyloliquefaciens , B. velezensis , and B. siamensis , including inulinase activity. Bacillus genus is considered of interest for its enzyme productivity for many manufacturing processes [ 33 , 34 , 35 ]. The antibiotic sensitivity testing revealed that Bacillus sp.…”

Section: Resultsmentioning

confidence: 99%

Representative Bacillus sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation

López-Moreno

Torres-Sánchez

Acuña³

et al. 2021

IJMS

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Human gut microbiota harbors numerous microbial species with molecular enzymatic potential that impact on the eubiosis/dysbiosis and health/disease balances. Microbiota species isolation and description of their specific molecular features remain largely unexplored. In the present study, we focused on the cultivation and selection of species able to tolerate or biodegrade the endocrine disruptor bisphenol A (BPA), a xenobiotic extensively found in food plastic containers. Chemical xenobiotic addition methods for the directed isolation, culturing, Whole Genome Sequencing (WGS), phylogenomic identification, and specific gene-encoding searches have been applied to isolate microorganisms, assess their BPA metabolization potential, and describe encoded catabolic pathways. BPA-tolerant strains were isolated from 30% of infant fecal microbial culture libraries analyzed. Most isolated strains were phylogenetically related to the operational taxonomic group Bacillus amyloliquefaciens spp. Importantly, WGS analysis of microbial representative strain, Bacillus sp. AM1 identified the four complete molecular pathways involved on BPA degradation indicating its versatility and high potential to degrade BPA. Pathways for Exopolysaccharide (EPS) and Polyhydroxyalkanates (PHA) biopolymer synthesis were also identified and phenotypically confirmed by transmission electronic microscopy (TEM). These microbial biopolymers could generally contribute to capture and/or deposit xenobiotics.

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“…The increased protease activity for FA100 and FA60 treatments may be associated with the capability of Bacillus sp. and Paenibacillus strains to produce heat stable proteases [53,54]. The limitations of phosphorus availability in soils are known to stimulate plant roots to secrete acid phosphatases which may contributed to an improvement in the aforementioned enzyme activity in AL soil in comparison with BA soil [55,56].…”

Section: Discussionmentioning

confidence: 99%

The Status of Soil Microbiome as Affected by the Application of Phosphorus Biofertilizer: Fertilizer Enriched with Beneficial Bacterial Strains

Mącik

Gryta

Sas-Paszt

et al. 2020

IJMS

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Regarding the unfavourable changes in agroecosystems resulting from the excessive application of mineral fertilizers, biopreparations containing live microorganisms are gaining increasing attention. We assumed that the application of phosphorus mineral fertilizer enriched with strains of beneficial microorganisms contribute to favourable changes in enzymatic activity and in the genetic and functional diversity of microbial populations inhabiting degraded soils. Therefore, in field experiments conditions, the effects of phosphorus fertilizer enriched with bacterial strains on the status of soil microbiome in two chemically degraded soil types (Brunic Arenosol – BA and Abruptic Luvisol – AL) were investigated. The field experiments included treatments with an optimal dose of phosphorus fertilizer (without microorganisms – FC), optimal dose of phosphorus fertilizer enriched with microorganisms including Paenibacillus polymyxa strain CHT114AB, Bacillus amyloliquefaciens strain AF75BB and Bacillus sp. strain CZP4/4 (FA100) and a dose of phosphorus fertilizer reduced by 40% and enriched with the above-mentioned bacteria (FA60). The analyzes performed included: the determination of the activity of the soil enzymes (protease, urease, acid phosphomonoesterase, β-glucosidase), the assessment of the functional diversity of microorganisms with the application of BIOLOGTM plates and the characterization of the genetic diversity of bacteria, archaea and fungi with multiplex terminal restriction fragment length polymorphism and next generation sequencing. The obtained results indicated that the application of phosphorus fertilizer enriched with microorganisms improved enzymatic activity, and the genetic and functional diversity of the soil microbial communities, however these effects were dependent on the soil type.

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“…Bacillus sp. AM1 and other strains belonging to Bacillus genus have shown remarkable hydrolytic enzyme capacity ( Table 3 ), being related to the performance of key roles in several biotechnological and many manufacturing processes [ 61 , 62 , 63 ].…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Effects of Potential Probiotics of Bacillus spp. Isolated from Human Microbiota: In Vitro and In Silico Methods

et al. 2021

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The variable taxa components of human gut microbiota seem to have an enormous biotechnological potential that is not yet well explored. To investigate the usefulness and applications of its biocompounds and/or bioactive substances would have a dual impact, allowing us to better understand the ecology of these microbiota consortia and to obtain resources for extended uses. Our research team has obtained a catalogue of isolated and typified strains from microbiota showing resistance to dietary contaminants and obesogens. Special attention was paid to cultivable Bacillus species as potential next-generation probiotics (NGP) together with their antimicrobial production and ecological impacts. The objective of the present work focused on bioinformatic genome data mining and phenotypic analyses for antimicrobial production. In silico methods were applied over the phylogenetically closest type strain genomes of the microbiota Bacillus spp. isolates and standardized antimicrobial production procedures were used. The main results showed partial and complete gene identification and presence of polyketide (PK) clusters on the whole genome sequences (WGS) analysed. Moreover, specific antimicrobial effects against B. cereus, B. circulans, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Serratia marcescens, Klebsiella spp., Pseudomonas spp., and Salmonella spp. confirmed their capacity of antimicrobial production. In conclusion, Bacillus strains isolated from human gut microbiota and taxonomic group, resistant to Bisphenols as xenobiotics type endocrine disruptors, showed parallel PKS biosynthesis and a phenotypic antimicrobial effect. This could modulate the composition of human gut microbiota and therefore its functionalities, becoming a predominant group when high contaminant exposure conditions are present.

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“…Microbial enzymes such as α-amylase, protease, and lipase have been used in various biotechnological applications including textile applications, feed industry, food industry, and organic synthesis [87][88][89]. The U.S. Food and Drug Administration (FDA) in 1999 reported that enzymes such as α-amylase and protease originating from B. subtilis are Generally Recognized as Safe (GRAS) for use as direct food ingredients [90].…”

Section: Source Of Commercial Enzymesmentioning

confidence: 99%

A Review on the Biotechnological Applications of the Operational Group Bacillus amyloliquefaciens

et al. 2021

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Bacteria under the operational group Bacillus amyloliquefaciens (OGBa) are all Gram-positive, endospore-forming, and rod-shaped. Taxonomically, the OGBa belongs to the Bacillus subtilis species complex, family Bacillaceae, class Bacilli, and phylum Firmicutes. To date, the OGBa comprises four bacterial species: Bacillus amyloliquefaciens, Bacillus siamensis, Bacillus velezensis and Bacillus nakamurai. They are widely distributed in various niches including soil, plants, food, and water. A resurgence in genome mining has caused an increased focus on the biotechnological applications of bacterial species belonging to the OGBa. The members of OGBa are known as plant growth-promoting bacteria (PGPB) due to their abilities to fix nitrogen, solubilize phosphate, and produce siderophore and phytohormones, as well as antimicrobial compounds. Moreover, they are also reported to produce various enzymes including α-amylase, protease, lipase, cellulase, xylanase, pectinase, aminotransferase, barnase, peroxidase, and laccase. Antimicrobial compounds that able to inhibit the growth of pathogens including non-ribosomal peptides and polyketides are also produced by these bacteria. Within the OGBa, various B. velezensis strains are promising for use as probiotics for animals and fishes. Genome mining has revealed the potential applications of members of OGBa for removing organophosphorus (OPs) pesticides. Thus, this review focused on the applicability of members of OGBa as plant growth promoters, biocontrol agents, probiotics, bioremediation agents, as well as producers of commercial enzymes and antibiotics. Here, the bioformulations and commercial products available based on these bacteria are also highlighted. This review will better facilitate understandings of members of OGBa and their biotechnological applications.

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“…The culture medium used in the protease production contained 0.5% glucose (w/v), 0.75% peptone (w/v), 0.5% (w/v) MgSO4.7H2O, 0.5% (w/v), KH2PO4 (w/v), and 0.01% (w/v) FeSO4.7H2OS (w/v) maintained at 37°C for 24 h with pH 6.8 in a shaking incubator at (140 rpm). At the end of each fermentation period, the entire fermentation broth was centrifuged at 10,000 rpm at 4°C for 15 min and the clear supernatant was used for crude enzyme preparation 16 .…”

Section: Protease Productionmentioning

confidence: 99%

Study of Uv Mutated Protease Enzyme From Various Sources

Saranya¹,

Dhanachezhiyan²,

Gopinath³

et al. 2019

Int Res J Pharm

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Proteases have wide variety of functions and commercial applications. Protease is the third largest groups of industrial enzymes and is used in detergents production, leather industry, food industry, pharmaceutical industry and bioremediation processes. Protease and amylase are well known industrially important enzymes. To study the UV mutated protease enzyme from various sources. Microbes were isolated from curd, buccal cavity are probiotics were maintained under optimum conditions, the microbial growth and enzyme production carried out at 37 º C for at 24 hours and pH 7.0. The isolated microbes were mutated using UV radiation exposure which enhanced protease and amylase enzymes production. The protease enzyme was confirmed using protease and amylase assay. The isolated bacteria have the ability to produce the industrially valuable enzymes which have various industrial applications. The protease enzyme has wide applications in many industries and therefore the UV mutated enzyme will have more thermostability.

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Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29

Cited by 13 publications

References 155 publications

Representative Bacillus sp. AM1 from Gut Microbiota Harbor Versatile Molecular Pathways for Bisphenol A Biodegradation

The Status of Soil Microbiome as Affected by the Application of Phosphorus Biofertilizer: Fertilizer Enriched with Beneficial Bacterial Strains

Antimicrobial Effects of Potential Probiotics of Bacillus spp. Isolated from Human Microbiota: In Vitro and In Silico Methods

A Review on the Biotechnological Applications of the Operational Group Bacillus amyloliquefaciens

Study of Uv Mutated Protease Enzyme From Various Sources

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