Production of tannase (tannin acyl hydrolase E.C. 3.1.1.20) by a strain of Aspergillus niger

Pourrat, Henri; Regerat, F; Pourrat, A; Jean, D.

doi:10.1007/bf00127789

Cited by 25 publications

(17 citation statements)

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“…3.1.1.20) catalyzes the hydrolysis of tannins by breaking their esters and depside bonds releasing glucose and gallic acid. At present, tannase is mostly used to manufacture instant tea, acorn liquor and gallic acid (Coggon et al 1975;Pourrat et al 1985). This product of tannin hydrolysis finds application in many fields like the dye-making, pharmaceutical, leather and chemical industries (Hadi et al 1994;Mukharjee and Banerjee 2003).…”

Section: Introductionmentioning

confidence: 96%

Production of tannase from wood-degrading fungus using as substrate plant residues: purification and characterization

Ordóñez

Colombo

Arena

et al. 2011

World J Microbiol Biotechnol

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In the present study Lenzites elegans, Schizophyllum commune, Ganoderma applanatum and Pycnoporus sanguineus (wood-degrading fungi) were assayed for their tannase producing potential in culture media containing plant residues or/and tannic acid as carbon source. Aspergillus niger was used as positive control for tannase production. We also carried out the isolation, purification and characterization of the enzyme from the fungi selected as the major productor. The highest fungal growth was observed in A. niger and L. elegans in the media containing tannic acid ? glucose ? plant residues (Fabiana densa). A. niger and L. elegans reached the highest extracellular tannase production in a medium containing tannic acid ? F. densa and in a medium supplemented with glucose ? tannic acid ? F. densa. The produced enzyme by L. elegans was purified by DEAE-Sepharose. Km value was 5.5 mM and relative molecular mass was about 163,000. Tannase was stable at a pH range 3.0-6.0 and its optimum pH was 5.5. The enzyme showed an optimum temperature of 60°C and was stable between 40 and 60°C. This paper is the first communication of tannase production by wood-degrading fungi. Fermentation technology to produce tannase using plant residues and xylophagous fungi could be very important in order to take advantage of plant industrial waste.

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Section: Introductionmentioning

confidence: 96%

Production of tannase from wood-degrading fungus using as substrate plant residues: purification and characterization

Ordóñez

Colombo

Arena

et al. 2011

World J Microbiol Biotechnol

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“…Seth and Chan [13] also explained that at lower agitation rates, the inadequate mixing of the broth towards the later stages of growth affected the enzyme synthesis by accumulation of gallic acid on the surface of the mycelium, while the drastic fall in enzyme activity at higher agitation rates was largely due to shearing effect on the mycelium. In the same way, Barthomeuf et al [14] and Pourrat et al [15,16] have found that excessive aeration favored the oxidation of tannins and thus had inhibiting effects on the biosynthesis of the tannase. Furthermore, changes in the morphology of microorganisms caused by different agitation speeds were also found to influence tannase production and growth of the organism [17,18].…”

Section: Discussionmentioning

confidence: 88%

Influence of Agitation Speed on Tannase Production and Morphology of Aspergillus niger FETL FT3 in Submerged Fermentation

Demirtaş

Sumathi

Jain

et al. 2011

Appl Biochem Biotechnol

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Agitation speed was found to influence the tannase production and fungal growth of Aspergillus niger FETL FT3. The optimal agitation speed was at 200 rpm which produced 1.41 U/ml tannase and 3.75 g/l of fungal growth. Lower or higher agitation speeds than 200 rpm produced lower enzyme production and fungal growth. Based on the SEM and TEM micrograph observation, there was a significant correlation between agitation speed and the morphology of the fungal mycelia. The results revealed an increase of the enzyme production with the change of the fungal growth morphology from filamentous to pelleted growth forms. However, the exposure to higher shear stress with an increasing agitation speed of the shaker also resulted in lower biomass yields as well as enzyme production.

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“…and Lonsane, 1997). Besides the SmF process, some studies have been done on the production of TAH by the LSF system (Ganga et al, 1977(Ganga et al, , 1978Pourrat et al, 1982). During the past decade, efforts have been intensified for production of TAH using the SSF system (Aguilar et al, 2001a(Aguilar et al, , b, 2002Favela-Torres et al, 1998;Kar and Banerjee, 2000;Ramirez-Coronel et al, 2003;Pyle, 2001, 2004).…”

Section: Discussionmentioning

confidence: 99%

Effect of fermentation system on the production and properties of tannase of Aspergillus niger van Tieghem MTCC 2425

Rana

Bhat

2005

J. Gen. Appl. Microbiol.

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The tannase-producing efficiency of liquid-surface fermentation (LSF) and solid-state fermentation (SSF) vis-à-vis submerged fermentation (SmF) was investigated in a strain of Aspergillus niger, besides finding out if there was a change in the activity pattern of tannase in these fermentation processes. The studies on the physicochemical properties were confined to intracellular tannase as only this form of enzyme was produced by A. niger in all three fermentation processes. In LSF and SmF, the maximum production of tannase was observed by 120 h, whereas in SSF its activity peaked at 96 h of growth. SSF had the maximum efficiency of enzyme production. Tannase produced by the SmF, LSF and SSF processes had similar properties except that the one produced during SSF had a broader pH stability of 4.5-6.5 and thermostability of 20°-60°C.

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Production of tannase (tannin acyl hydrolase E.C. 3.1.1.20) by a strain of Aspergillus niger

Abstract: Culture of a strain of Aspergillus niger on medium containing3 % tannic acid yielded tannase (tanin acylhydrolase), evidenced both in the culture medium and the mycelium. Fermentation in submerged culture at constan: air flow gave mycelium with high tannase activity.

Cited by 25 publications

References 1 publication

Production of tannase from wood-degrading fungus using as substrate plant residues: purification and characterization

Production of tannase from wood-degrading fungus using as substrate plant residues: purification and characterization

Influence of Agitation Speed on Tannase Production and Morphology of Aspergillus niger FETL FT3 in Submerged Fermentation

Effect of fermentation system on the production and properties of tannase of Aspergillus niger van Tieghem MTCC 2425

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