Influence of Agitation Speed on Tannase Production and Morphology of Aspergillus niger FETL FT3 in Submerged Fermentation

Demirtaş, İbrahim; Sumathi, G.; Jain, Kavish Kumar; Lim, Sheh-Hong

doi:10.1007/s12010-011-9387-8

Cited by 24 publications

(20 citation statements)

References 16 publications

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“…5A. However, higher Re values resulted in higher shear rate that could break down the flocculation of the sludge, inhibit the biomass activity, reduce biomass concentration and change the metabolic distributions, etc [25,26]. Therefore, the hydrogen yields showed no notable increase anymore at higher Re values, with the values fluctuated between 1.9 and 2.0 mol/mol_glucose.…”

Section: 2mentioning

confidence: 99%

Hydrogen supersaturation in thermophilic mixed culture fermentation

Zhang

Chen

et al. 2012

International Journal of Hydrogen Energy

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Section: 2mentioning

confidence: 99%

Hydrogen supersaturation in thermophilic mixed culture fermentation

Zhang

Chen

et al. 2012

International Journal of Hydrogen Energy

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“…The samples for scanning electron microscopy (SEM) were prepared as described previously by Darah et al [5] and…”

Section: Scanning Electron Microscopy Studiesmentioning

confidence: 99%

“…Agitation speed and dissolved oxygen concentration on pectinase production are important factors leading to successful progress of fermentation. Sufficient dissolved oxygen in medium is an important for microbial cells in mass transfer characteristics example substrate and product [5] . Agitation provides adequate mixing, mass and heat transfer.…”

Section: A B C D E Fmentioning

confidence: 99%

“…Agitation provides adequate mixing, mass and heat transfer. However, agitation also creates shear forces, causing morphological changes, and eventually damage to the cell structure [5,20] . In industrial applications submerged cultures of filamentous fungi are widely used to produce com mercially products and pellet form is usually preferred in downstream fermentation process due to the non viscosity of fermentation medium [14] .…”

Section: A B C D E Fmentioning

confidence: 99%

“…The rate of the agitation speed could influenced the extent of mixing in the shake flasks system and affected the nutrient availability as well [4] . Agitation speed has been affected many enzymes activities in different strains of bacteria and fungi [5,6] as well as microalgae [7] . Agitation provides adequate mixing, mass, heat transfer and also improving dissolved oxygen in the culture medium.…”

Section: Introductionmentioning

confidence: 99%

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Effect of agitation speed on the morphology ofAspergillus nigerHFD5A-1 hyphae and its pectinase production in submerged fermentation

Demirtaş

Weloosamy

Lim

2015

WJBC

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AIM:To investigate the impact of agitation speed on pectinase production and morphological changing of Aspergillus niger (A. niger ) HFD5A-1 in submerged fermentation. METHODS:A. niger HFM5A-1 was isolated from a rotted pomelo. The inoculum preparation was performed by adding 5.0 mL of sterile distilled water containing 0.1% Tween 80 to a sporulated culture. Cultivation was carried out with inoculated 1 × 10 7 spores/mL suspension and incubated at 30 ℃ with different agitation speed for 6 d. The samples were withdrawn after 6 d cultivation time and were assayed for pectinase activity and fungal growth determination. The culture broth was filtered through filter paper (Whatman No. 1, London) to separate the fungal mycelium. The cell-free culture filtrate containing the crude enzyme was then assayed for pectinase activity. The biomass was dried at 80 ℃ until constant weight. The fungal cell dry weight was then expressed as g/L. The 6 d old fungal mycelia were harvested from various agitation speed, 0, 50, 100, 150, 200 and 250 rpm. The morphological changing of samples was then viewed under the light microscope and scanning electron microscope. RESULTS:In the present study, agitation speed was found to influence pectinase production in a batch cultivation system. However, higher agitation speeds than the optimal speed (150 rpm) reduced pectinase Effect of agitation speed on the morphology of Aspergillus niger HFD5A-1 hyphae and its pectinase production in submerged fermentation ORIGINAL ARTICLE Basic StudyDarah Ibrahim, Haritharan Weloosamy, Sheh-Hong Lim production which due to shear forces and also collision among the suspended fungal cells in the cultivation medium. Enzyme activity increased with the increasing of agitation speed up to 150 rpm, where it achieved its maximal pectinase activity of 1.559 U/mL. There were significant different (Duncan, P < 0.05) of the pectinase production with the agitation speed at static, 50, 100, 200 and 250 rpm. At the static condition, a well growth mycelial mat was observed on the surface of the cultivation medium and sporulation occurred all over the fungal mycelial mat. However with the increased in agitation speed, the mycelial mat turned slowly to become a single circular pellet. Thus, it was found that agitation speed affected the morphological characteristics of the fungal hyphae/mycelia of A. niger HFD5A-1 by altering their external as well as internal cell structures. CONCLUSION:Exposure to higher shear stress with an increasing agitation speed could result in lower biomass yields as well as pectinase production by A. niger HFD5A-1. Core tip:We report the influence of agitation speed on pectinase production on our newly isolate Aspergillus niger (A. niger ) HFM5A-1 in submerged fermentation.The agitation speed was found to influence pectinase production in a batch cultivation system. However, higher agitation speeds than the optimal speed (150 rpm) reduced pectinase production which due to shear forces and also collision among the suspended fungal cells in...

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Polygalacturonase Production by Calcium Alginate Immobilized Enterobacter aerogenes NBO2 Cells

Demirtaş

Nisha

Lim

2014

Appl Biochem Biotechnol

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Bacterial cells of Enterobacter aerogenes NBO2 were entrapped in calcium alginate beads in order to enhance polygalacturonase production compared to free cells. The optimized condition of 5 % (w/v) sodium alginate concentration, agitation speed of 250 rpm, and 15 beads of calcium alginate with inoculum size of 4 % (v/v; 5.4 × 10(7) cells/ml) produced 23.48 U/mL of polygalacturonase compared to free cells of 18.54 U/ml. There was about 26.6 % increment in polygalaturonase production. However, in this study, there was 296.6 % of increment in polygalacturonase production after improvement parameters compared to before improvement parameters of calcium alginate bead immobilization cells (5.92 U/ml). This research has indicated that optimized physical parameters of calcium alginate bead immobilization cells have significantly enhanced the production of polygalacturonase.

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Influence of Agitation Speed on Tannase Production and Morphology of Aspergillus niger FETL FT3 in Submerged Fermentation

Cited by 24 publications

References 16 publications

Hydrogen supersaturation in thermophilic mixed culture fermentation

Hydrogen supersaturation in thermophilic mixed culture fermentation

Effect of agitation speed on the morphology ofAspergillus nigerHFD5A-1 hyphae and its pectinase production in submerged fermentation

Polygalacturonase Production by Calcium Alginate Immobilized Enterobacter aerogenes NBO2 Cells

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