Polygalacturonase Production by Calcium Alginate Immobilized Enterobacter aerogenes NBO2 Cells

Demirtaş, İbrahim; Nisha, Maimun; Lim, Sheh-Hong

doi:10.1007/s12010-014-1447-4

Cited by 12 publications

(8 citation statements)

References 8 publications

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“…Only Bacillus spp isolated where indicated to be a producer of pectinase. This agrees with different documentations of Bayoumi et al, Geetha [75][76][77][78][79][80][81][82]. Bacillus subtilis being a producer of pectinase agrees with the report of Mariam and Aruna that B. subtilis strain arium 1115 produced the highest quantity of extracellular pectinase out of the arrays of Bacillus spp assayed for [83] [80][81][82][83][84][85][86][87][88][89].…”

Section: Discussionsupporting

confidence: 90%

Millet Cobs: A Source of Microbial Enzymes

Ao¹,

Dj²

2018

J Microb Biochem Technol

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Section: Discussionsupporting

confidence: 90%

Millet Cobs: A Source of Microbial Enzymes

Ao¹,

Dj²

2018

J Microb Biochem Technol

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“…IFVB and Citrobacter sp. IFVB were greater than reported (18Á54 and 1Á047 U ml À1 respectively) (Darah et al 2015;Tripathi and Kapoor 2018).…”

Section: Discussioncontrasting

confidence: 63%

Evaluation of process involved in the production of aromatic compounds in Gram‐negative bacteria isolated from vanilla ( Vanilla planifolia ex. Andrews) beans

et al. 2019

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Aim The present investigation was aimed at isolating and identifying bacterial strains from cured vanilla beans. Additionally, the study focused on evaluating bacterial processes pertaining to the aromatic compounds production (ACP). Methods and Results Three bacteria were isolated from Vanilla planifolia beans, previously subjected to the curing process. According to morphological, biochemical and 16S rRNA analysis, the strains were identified as Citrobacter sp., Enterobacter sp. and Pseudomonas sp. The polygalacturonase activity (PGA) was determined using the drop, cup‐plate and DNS methods. Aromatic compounds production was analysed by cup‐plate method using FA as substrate and quantified by high performance liquid chromatography (ppm), the functional groups of vanillic acid (VA) were identified by FT‐IR and the aromatic compounds (AC) resistance was determined and reported as minimum inhibitory concentration. Citrobacter sp., Enterobacter sp. and Pseudomonas showed PGA (70·31 ± 364, 76·07 ± 12·47 and 51 ± 10·92 U ml−1 respectively), were producers of VA (3·23 ± 0·49, 324 ± 41 and 265·99 ± 11·61 ppm respectively) and were resistant to AC. Conclusions The Gram‐negative bacteria isolated from V. planifolia beans were responsible for ACP. Significance and Impact of the Study This is the first evidence for the role of Gram‐negative bacterial isolates from cured Mexican V. planifolia beans in the process related to ACP.

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“…Pseudomonas aeruginosa (ZS-45), isolated from orange produced maximum enzyme activity, 4.4 U/µL. Polygalacturonase production by Enterobacter aerogenes NBO 2 (18.54 U/mL) ( 17 ) was much less compared to Bacillus and Pseudomonas (4.4 U/µL each). Zeni et al ( 18 ) reported the same results for PG production by various species of Bacillus from agro-industrial waste.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis of Polygalacturonase-Producing Bacillus and Pseudomonas Isolated From Plant Waste Material

Sohail

Latif

2016

Jundishapur J Microbiol

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Background:Keeping in mind the commercial application of polygalacturonase (PG) in juice and beverages industry, bacterial strains were isolated from rotten fruits and vegetables to screen for competent producers of PG.Objectives:In this study, the plate method was used for preliminary screening of polygalacturonase-producing bacteria, while the Dinitrosalicylic Acid (DNS) method was used for quantifications of PG.Materials and Methods:Biochemically-identified polygalacturonase-producing Bacillus and Pseudomonas species were further characterized by molecular markers. The genetic diversity among these selected strains was analyzed by investigating microsatellite distribution in their genome. Out of 110 strains, 17 competent strains of Bacillus and eight strains of Pseudomonas were selected, identified and confirmed biochemically. Selected strains were characterized by 16S rRNA sequencing and data was submitted to the national center for biotechnology information (NCBI) website for accession numbers.Results:Among the Bacillus, Bacillus vallismortis (JQ990307) isolated from mango was the most competent producer of PG; producing up to 4.4 U/µL. Amongst Pseudomonas, Pseudomonas aeruginosa (JQ990314) isolated from oranges was the most competent PG producer equivalent to B. vallismortis (JQ990307). To determine genetic diversity of different strains of Pseudomonas and Bacillus varying in PG production, fingerprinting was done on the basis of Simple Sequence Repeats (SSR) or microsatellites. The data was analyzed and a phylogenetic tree was constructed using the Minitab 3 software for comparison of bacterial isolates producing different concentrations of PG. Fingerprinting showed that presence or absence of certain microsatellites correlated with the ability of PG production.Conclusions:Bacteria from biological waste were competent producers of PG and must be used on an industrial scale to cope with the demand of PG in the food industry.

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Polygalacturonase Production by Calcium Alginate Immobilized Enterobacter aerogenes NBO2 Cells

Cited by 12 publications

References 8 publications

Millet Cobs: A Source of Microbial Enzymes

Millet Cobs: A Source of Microbial Enzymes

Evaluation of process involved in the production of aromatic compounds in Gram‐negative bacteria isolated from vanilla ( Vanilla planifolia ex. Andrews) beans

Phylogenetic Analysis of Polygalacturonase-Producing Bacillus and Pseudomonas Isolated From Plant Waste Material

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