Hydrogen supersaturation in thermophilic mixed culture fermentation

Zhang, Fang; Zhang, Yan; Chen, Man; Zeng, Raymond J.

doi:10.1016/j.ijhydene.2012.09.019

Cited by 53 publications

(32 citation statements)

References 27 publications

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“…hydrogenase concentration) was greater with 4 g/L glucose. This result was consistent with other studies [14,28].…”

Section: The Effect Of Dh On Decolorizationsupporting

confidence: 94%

“…Concentrations of DH were determined by MIMS, which was calibrated by a standard calibration method [14]. Briefly, the recorded mass to charge (m/z) ratio of H 2 was 2 for the MIMS signal.…”

Section: Discussionmentioning

confidence: 99%

“…The function of hydrogenase in the decolorization process, however, is not fully understood [8,13]. By contrast, H 2 supersaturation is widely observed in mixed culture fermentation [14,15]; thus, DH in liquid is more meaningful than H 2 in the headspace to represent hydrogen for the metabolic process.…”

Section: Introductionmentioning

confidence: 99%

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Decolorization by Caldicellulosiruptor saccharolyticus with dissolved hydrogen under extreme thermophilic conditions

Shen

Huo

Chen

et al. 2015

Chemical Engineering Journal

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“…hydrogenase concentration) was greater with 4 g/L glucose. This result was consistent with other studies [14,28].…”

Section: The Effect Of Dh On Decolorizationsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Decolorization by Caldicellulosiruptor saccharolyticus with dissolved hydrogen under extreme thermophilic conditions

Shen

Huo

Chen

et al. 2015

Chemical Engineering Journal

Self Cite

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“…For glucose, the data was obtained from mixed culture dark fermentation under continuous mode with suspended microorganisms, i.e. not immobilized (Aceves-Lara et al 2008a, b;Temudo et al 2008aTemudo et al , b, 2009Bastidas-Oyanedel et al 2012;Zhang et al 2012). In the case of organic solid waste the data corresponds to the dark fermentation of organic fraction of municipal solid waste (Zahedi et al 2013) and food waste (Jiang et al 2013).…”

Section: Dark Fermentation Product Considerationsmentioning

confidence: 99%

“…Nowadays these compounds are produced by petroleum-based chemical synthesis, Temudo et al 2008aTemudo et al , 2008bTemudo et al , 2009BastidasOyanedel et al 2012;Zhang et al 2012). Organic solid waste plot is based on Jiang et al (2013) and Zahedi et al (2013) Mussatto et al (2010) Rev Environ Sci Biotechnol except for ethanol and lactic acid which are mainly produced by pure-culture bioprocess.…”

Section: Technological Importance Of Dark Fermentation Productsmentioning

confidence: 99%

Dark fermentation biorefinery in the present and future (bio)chemical industry

Bastidas‐Oyanedel

Bonk

Thomsen

et al. 2015

Rev Environ Sci Biotechnol

133

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Dark fermentation, also known as acidogenesis, involves the transformation of a wide range of organic substrates into a mixture of products, e.g. acetic acid, butyric acid and hydrogen. This bioprocess occurs in the absence of oxygen and light. The ability to synthesize hydrogen, by dark fermentation, has raised its scientific attention. Hydrogen is a nonpolluting energy carrier molecule. However, for energy generation, there is a variety of other sustainable alternatives to hydrogen energy, e.g. solar, wind, tide, hydroelectric, biomass incineration, or nuclear fission. Nevertheless, dark fermentation appears as an important sustainable process in another area: the synthesis of valuable chemicals, i.e. an alternative to petrochemical refinery. Currently, acetic acid, butyric acid and hydrogen are mostly produced by petrochemical reforming, and they serve as precursors of ubiquitous petrochemical derived products. Hence, the future of dark fermentation relies as a core bioprocess in the biorefinery concept. The present article aims to present and discuss the current and future status of dark fermentation in the biorefinery concept. The first half of the article presents the metabolic pathways, product yields and its technological importance, microorganisms responsible for mixed dark fermentation, and operational parameters, e.g. substrates, pH, temperature and head-space composition, which affect dark fermentation. The minimal selling price of dark fermentation products is also presented in this section. The second half discusses the perspectives and future of dark fermentation as a core bioprocess. The relationship of dark fermentation with other (bio)processes, e.g. liquid fuels and fine chemicals, algae cultivation, biomethane-biohythane-biosyngas production, and syngas fermentation, is then explored.

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Overflow metabolism and growth cessation in Clostridium thermocellum DSM1313 during high cellulose loading fermentations

Thompson

Trinh

2017

Biotech & Bioengineering

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As a model thermophilic bacterium for the production of second-generation biofuels, the metabolism of Clostridium thermocellum has been widely studied. However, most studies have characterized C. thermocellum metabolism for growth at relatively low substrate concentrations. This outlook is not industrially relevant, however, as commercial viability requires substrate loadings of at least 100 g/L cellulosic materials. Recently, a wild-type C. thermocellum DSM1313 was cultured on high cellulose loading batch fermentations and reported to produce a wide range of fermentative products not seen at lower substrate concentrations, opening the door for a more in-depth analysis of how this organism will behave in industrially relevant conditions. In this work, we elucidated the interconnectedness of overflow metabolism and growth cessation in C. thermocellum during high cellulose loading batch fermentations (100 g/L). Metabolic flux and thermodynamic analyses suggested that hydrogen and formate accumulation perturbed the complex redox metabolism and limited conversion of pyruvate to acetyl-CoA conversion, likely leading to overflow metabolism and growth cessation in C. thermocellum. Pyruvate formate lyase (PFL) acts as an important redox valve and its flux is inhibited by formate accumulation. Finally, we demonstrated that manipulation of fermentation conditions to alleviate hydrogen accumulation could dramatically alter the fate of pyruvate, providing valuable insight into process design for enhanced C. thermocellum production of chemicals and biofuels. Biotechnol. Bioeng. 2017;114: 2592-2604. © 2017 Wiley Periodicals, Inc.

show abstract

Hydrogen supersaturation in thermophilic mixed culture fermentation

Cited by 53 publications

References 27 publications

Decolorization by Caldicellulosiruptor saccharolyticus with dissolved hydrogen under extreme thermophilic conditions

Decolorization by Caldicellulosiruptor saccharolyticus with dissolved hydrogen under extreme thermophilic conditions

Dark fermentation biorefinery in the present and future (bio)chemical industry

Overflow metabolism and growth cessation in Clostridium thermocellum DSM1313 during high cellulose loading fermentations

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