Effect of fermentation system on the production and properties of tannase of Aspergillus niger van Tieghem MTCC 2425

Rana, Nisha; Bhat, T. K.

doi:10.2323/jgam.51.203

Cited by 38 publications

(23 citation statements)

References 37 publications

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“…The optimum temperature was determined by incubating the reaction mixture for 15 min at different temperatures (30,40,50,60,70,80, and 90°C). The substrate solution (0.01 M methyl gallate in 50 mM phosphate buffer pH 7) and 50 mM phosphate buffer were preincubated at the different temperatures before beginning the enzymatic reaction.…”

Section: Enzyme Stabilitymentioning

confidence: 99%

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Differential Properties of Aspergillus niger Tannase Produced Under Solid-State and Submerged Fermentations

Renovato

Gutiérrez-Sánchez

Rodríguez‐Durán

et al. 2011

Appl Biochem Biotechnol

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Significant differences on structure, stability, and catalytic properties of tannase were found when this enzyme was produced under solid-state and submerged fermentations (SSF and SmF) by Aspergillus niger. The specific activity was 5.5 times higher on SSF than in SmF. Significant differences in isoelectric points of tannases were found. The pH optima for both types of enzyme was found at 6 and the pH stability of SSF and SmF tannase were at 6 and 5-8, respectively. The optimal temperature range was from 50 to 60 °C for SmF tannase and 60 °C for SSF tannase, and both enzyme types showed tolerance to high temperatures (60-70 °C). The SSF tannase showed a major specificity for methyl gallate substrate while SmF tannase for tannic acid. All metal ions tested, had an activity inhibition from 30-46% on SSF tannase. SDS-PAGE analysis as well as gel localization studies of both SSF and SmF purified tannases showed a single band with a molecular weight of 102 and 105 kDa, respectively. Different levels of glycosylation were found among SSF and SmF purified tannases. This is the first report about structural differences among tannase produced under SSF and SmF and this study provides basis for explanation of the stability and catalytic differences observed previously for this two tannase types.

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Section: Enzyme Stabilitymentioning

confidence: 99%

“…At industrial levels tannase is mainly produced by Aspergillus species under submerged fermentation (SmF). The drawback of tannase produced under SmF, is that it is expressed mainly intracellularly by fungi and additional costs for purification need to be considered [30,40].…”

Section: Introductionmentioning

confidence: 99%

Differential Properties of Aspergillus niger Tannase Produced Under Solid-State and Submerged Fermentations

Renovato

Gutiérrez-Sánchez

Rodríguez‐Durán

et al. 2011

Appl Biochem Biotechnol

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“…They are water-soluble polyphenols with molecular mass ranging from 0.3 to 5 kDa (Bhat et al 1998;Rana et al 2005). Tannase (Tannin acyl hydrolase, E.C.3.1.1.20) is an industrially important inducible enzyme produced by large number of fungi and a few bacteria which hydrolyzes the ester and depside bonds of tannin and gallic acid esters.…”

Section: Introductionmentioning

confidence: 99%

Tannase production by Aspergillus fumigatus MA under solid-state fermentation

Manjit

Yadav

Aggarwal

et al. 2008

World J Microbiol Biotechnol

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A tannase yielding fungal culture identified as Aspergillus fumigatus MA was isolated from the effluent collected from a local small scale tannery. The fungal culture produced high yields of extracellular tannase under solid-state fermentation (SSF) using different agro forest residues such as Amla leaves (Phyllanthus emblica), Ber leaves (Zyzyphus mauritiana), Jamun leaves (Syzygium cumini), Jamoa leaves (Syzygium sp.) and Keekar leaves (Acacia nilotica). Among different substrates used, Jamun leaves yielded maximal extra-cellular production of tannase. Various parameters were studied to optimize the extracellular yield of tannase under SSF. The maximum yield of 174.32 U g -1 was obtained at 25°C after 96 h of incubation at pH 5.0. The tap water was used as a moistening agent. A substrate to tap water ratio of 1:1 was found to best for tannase production. Supplementation of the medium with ammonium sulfate as nitrogen source had enhanced tannase production whereas glucose had decreased the enzyme production. This is the first report on production of tannase by Aspergillus fumigatus MA, giving a much higher yield of enzyme under SSF with Jamun leaves as the substrate.

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“…Different optimum incubation periods, however, were reported by Alberts (2002) and Rana and Bhat (2005). Alberts (2002) found that optimum incubation period was 24 h while Rana and Bhat (2005) reported maximum tannase production with incubation period of 96 h. The varied optimal incubation period found by different workers might be due to differences in fungal strain and medium composition used for tannase production.…”

Section: Discussionmentioning

confidence: 65%

The Production of Tannin Acyl Hydrolase from Aspergillus niger

Anwar¹,

Hasim²,

Artika³

2007

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The aim of this research was to produce tannin acylhydrolase (tannase) from Aspergillus niger isolated from cacao pod. The first step of the study included determination of optimal pH, temperature, and incubation period to produce tannase. Optimal conditions obtained for tannase production were pH 5.5, a temperature of 28 o C and an incubation period of 3 days. Optimization of production medium was conducted. The media tested were solid and liquid wheat flour media with a concentration of tannic acid as inducer at 0, 3, 5, and 7% (wt/vol). The best production medium was solid medium with tannic acid concentration of 5% (wt/vol).

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Effect of fermentation system on the production and properties of tannase of Aspergillus niger van Tieghem MTCC 2425

Cited by 38 publications

References 37 publications

Differential Properties of Aspergillus niger Tannase Produced Under Solid-State and Submerged Fermentations

Differential Properties of Aspergillus niger Tannase Produced Under Solid-State and Submerged Fermentations

Tannase production by Aspergillus fumigatus MA under solid-state fermentation

The Production of Tannin Acyl Hydrolase from Aspergillus niger

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