Production of sophorolipids from whey: development of a two-stage process with Cryptococcus curvatus ATCC 20509 and Candida bombicola ATCC 22214 using deproteinized whey concentrates as substrates

Abstract

In order to produce sophorolipids from whey, thereby lowering the lactose content and biological oxygen demand, a two-step batch cultivation process was developed including medium sterilization by filtration. In the first step, whey was sterilized by a combination of crossflow and sterile filtration. Because the sophorolipid-producing yeast Candida bombicola ATCC 22214 was not able to use lactose as a carbon source directly, the oleaginous yeast Cryptococcus curvatus ATCC 20509 was grown on deproteinized whey concentrates (DWC). With 1:1 diluted DWC-20, lactose was consumed as the carbon source and biomass (24 g/l dry weight content) as well as single-cell oil (SCO, 10 g/l) were produced. The cultivation broth was disrupted with a glass bead mill and it served as medium for growth (29 g cell dry mass/l) and sophorolipid production (12 g/l) of the yeast C. bombicola.

Getting Lipids for Biodiesel Production from Oleaginous Fungi

“…Carbon sources other than glucose, such as xylose (Chistopher et al, 1983;Heredia & Ratledge, 1988;), lactose (Christopher et al, 1983;Daniel et al, 1999;), arabinose, mannose (Hansson & Dostalek, 1986), mannitol (Hansson & Dostalek, 1986), ethanol (Chistopher et al, 1983Eroshin & Krylova, 1983), have been also investigated in the 80s and 90s for the production of microbial lipids. Albeit glucose is a very good carbon source for lipid production with oleaginous fungi, molasses, which carbohydrate fraction is mainly composed of sucrose, glucose, and fructose, do not represent a promising raw material for lipid production, since they are characterized by a high nitrogen content which delays the unbalanced growth, where number of cells can not augment anymore and lipids are accumulated (Johnson et al, 1995).…”

Industrial Use of Whey by Fermentation Processes

“…Entre otros productos que se pueden obtener a partir de lactosuero están: insecticidas, como Endotoxina [77] o Toxina Mosquitocidal [78]; Solventes como el Acetano-Butanol [79], Acetona-Butanol-Etanol (ABE) [80], [81], [82]; Tensoactivos, como los Soforolípidos [83], [84], [85] y Vitaminas, como Vitamina C o Ácido L-Ascórbico [86] y Vitamina B12 [87], [88].…”

Production and stability studies of the bioemulsifier obtained from a new strain of Candida glabrata UCP 1002

“…Bioshyntesis of biosurfactants from a variety of bacteria and yeasts has been reported (Davila et al 1992;Zhou and Kosaric, 1995;Daniel et al 1999;Lang and Wullbrandt, 1999), most commonly involving rhamno-lipids, trehalose and sophorose-lipids. These usually contain various hydroxy fatty acids and carbohydrates and are characterized by unique surfactant properties (Ron and Rosenberg, 2001).…”