Getting Lipids for Biodiesel Production from Oleaginous Fungi

Rossi, Maddalena; Amaretti, Alberto; Raimondi, Stefano; Leonardi, Alan

doi:10.5772/25864

Cited by 49 publications

(50 citation statements)

References 86 publications

(73 reference statements)

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“…More than 75% of the obtained isolates were characterized as oleaginous according to Li el al. [4], since they were capable of yielding lipid content higher than 20% on their dry weight, These results confirm the wide distribution of these microorganisms as stated by Rossi et al [19]. Moreover, two of the psycrotrophic isolates from Antarctica resulted oleaginous.…”

Section: Discussionsupporting

confidence: 87%

Oleaginous Yeasts form Uruguay and Antarctica as Renewable Raw Material for Biodiesel Production

Pereyra¹

2014

AJBIO

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Abstract:In the present study a screening of oleaginous yeasts from different habitats nearby Montevideo, Uruguay, was carried out. Four yeast strains previously isolated from soil from Fildes Peninsula, in the Antarctic region, were also included in the study. More than 75% of the obtained isolates were characterized as oleaginous though only four of them (two from Antarctica and two from Uruguay) were able to accumulate lipids to levels exceeding the 40% of cell dry weight. One of the selected strains was identified as Rhodotorula graminis, which has been already recognized as an oleaginous species. However, the other three isolates belong to species, which have not been reported as oleaginous before. This work constitutes the first report of Cryptococcus phenolicus, Cystofilobasidium infirmominiatum and Leucosporidium scottii as oleaginous species. Three of the selected isolates were able to grow with glycerol as carbon source. According to lipid production in presence of glycerol, one isolate was selected for further studies. The ability to grow and accumulate intracellular lipids in presence of crude glycerol was assessed for the selected strain. In such conditions, a maximum concentration of 5.9 g L -1 of lipids with a suitable fatty acid profile according to the requirements established in our country for the raw material used in the production of biodiesel, was obtained.

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Section: Discussionsupporting

confidence: 87%

Oleaginous Yeasts form Uruguay and Antarctica as Renewable Raw Material for Biodiesel Production

Pereyra¹

2014

AJBIO

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“…5) via the ATP:citrate lyase (ACL) pathway. 3,4,[8][9][10]23 As abovementioned, with genetic modication, lipid accumulation can increase by as much as 90% of oleaginous yeast biomass . 10 Our ndings of over 70% lipid accumulation without genetic modication are really interesting in this context.…”

Section: View Article Onlinementioning

confidence: 98%

“…3,4,7 Research has been carried out in many directions, including molecular modication of the genes involved in lipid metabolism, to increase the lipid accumulation capacity of this yeast. [8][9][10] The leucine and uracil-supplemented complex medium for the leucine + uracil + strain of Y. lipolytica can produce high intracellular lipid, nearly 90% of the dry biomass. 10 However, literature on the metabolic modication of Y. lipolytica without genetic modication and cultivation with glycerol is rare.…”

Section: Introductionmentioning

confidence: 99%

Development of nutrient media to increase the accumulation of lipids without genetic modification of a lipogenic microorganism

et al. 2017

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a Yarrowia lipolytica has drawn attention from the scientists over the last few decades because of its potential to accumulate intracellular lipid that can be transesterified to methyl esters with properties resembling those of diesel. A kinetics study of intracellular lipid production in Y. lipolytica is very important because it would help process modeling and cost minimization for increasing the lipid production. A set of defined media with varying amounts of pure glycerol as the carbon source and a fixed amount of ammonium sulfate as the nitrogen source and other media components, including thiamine and uracil supplements, was used to study the kinetics of lipid production. With an increase in the carbon to nitrogen (C/N) ratio, lipid accumulation increased to a certain limit up to 4 g L À1, followed by substrate inhibition. The best rate and amount of lipid accumulation was predicted to be obtained in a cultivation medium with an initial glycerol concentration of 93 g L À1. While lipid production was high, citric acid production in the media was negligible. Minimization of citric acid synthesis via proper media development with nitrogen starvation and unique supplement utilization can increase lipid accumulation in Y. lipolytica IMUFRJ 50682 up to a great extent in mere batch cultivation without any genetic modification.

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“…Lipid accumulation occurred at the C/N ratio of 11.7-47 when citric acid production was absent or limited (Ochoa-Estopier and Guillouet, 2014). Higher ratios may reduce lipid yield and increase citric acid and other undesired byproducts (Rossi et al, 2011;Thevenieau and Nicaud, 2013). However, another study found that total glucose amount plays a major role in lipid accumulation for engineered glucose-grown strains (Blazeck et al, 2014).…”

Section: Strategies For Cultivation and Fermentationmentioning

confidence: 98%

Yarrowia lipolytica as an Oleaginous Cell Factory Platform for Production of Fatty Acid-Based Biofuel and Bioproducts

2014

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Today's biotechnologists seek new biocatalysts to meet the growing demand for the bioproducts. This review critically evaluates the potential use of Y. lipolytica as an oleaginous cell factory platform. This yeast has undergone extensive modifications for converting a wide range of hydrophobic and hydrophilic biomass, including alkane, oil, glycerol, and sugars to fatty acid-based products. This article highlights challenges in the development of this platform and provides an overview of strategies to enhance its potential in the sustainable production of biodiesel, functional dietary lipid compounds, and other value-added oleochemical compounds. Future applications of the recombinant Y. lipolytica platform are also discussed.

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Getting Lipids for Biodiesel Production from Oleaginous Fungi

Cited by 49 publications

References 86 publications

Oleaginous Yeasts form Uruguay and Antarctica as Renewable Raw Material for Biodiesel Production

Oleaginous Yeasts form Uruguay and Antarctica as Renewable Raw Material for Biodiesel Production

Development of nutrient media to increase the accumulation of lipids without genetic modification of a lipogenic microorganism

Yarrowia lipolytica as an Oleaginous Cell Factory Platform for Production of Fatty Acid-Based Biofuel and Bioproducts

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