Development of nutrient media to increase the accumulation of lipids without genetic modification of a lipogenic microorganism

Das, Shrilekha; Coelho, Maria Alice Zarur; Amaral, Priscilla Filomena Fonseca; Sil, Jayeeta

doi:10.1039/c7ra04026a

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…In this study, the lipid productivity was 1.62±0.11 g/L, without optimization. This lipid productivity is comparable with the results reported by Dobrowolski et al [37], who cultured Y. lipolytica on crude glycerol but lower than reported by Das et al [38], who reported lipid productivity of up to 4 g/L. A much higher lipid productivity of 11.02-16.45 (g/L) was observed when using vegetable oil to cultivate Y. lipolytica YB 423-12 [39].…”

Section: Nutritional Composition Of Yeast Biomass Cultured On Tuna He...supporting

confidence: 88%

Investigation of the Yeast Yarrowia lipolytica Cultivation on Tuna Head Protein Hydrolysate

Ngoc Thi Minh Ta,

Nguyen Hong Ngan,

Pham Thi Lan

2023

Curr. Appl. Sci. Technol.

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Tuna (Thunnus spp.) is a significant commodity within the fishing industry. However, up to half of the total tuna weight ends up as waste, including tuna heads, which contain valuable nutrients such as protein, polyunsaturated fatty acids, enzymes, minerals, and trace elements. The purpose of this study was to investigate an environmentally sustainable approach for tuna head processing by yeast cultivation. The oleaginous yeast Yarrowia lipolytica was successfully cultured on tuna head protein hydrolysate and the effects of culture conditions including inoculum size, hydrolysate pH and incubation volume on nutrition utilization and on yeast growth were investigated. Y. lipolytica showed good biomass accumulation when cultured on tuna head protein hydrolysates. The yeast biomass increased with decreasing incubation volume and reached 8.6 g/L. The yeast also showed the ability to reduce up to 83.7% of hydrolysate lipid content while remaining 70.7% of nitrogen content. The culture conditions had more effect on lipid reduction than on nitrogen reduction and a good correlation between the specific growth rate and lipid reduction was observed. The yeast biomass was 64.0% in protein content and 9.6% in lipid content, without optimization. The investigation results demonstrated the potential improvement of tuna head utilization as well as the potential application of yeast biomass cultivation.

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Section: Nutritional Composition Of Yeast Biomass Cultured On Tuna He...supporting

confidence: 88%

Investigation of the Yeast Yarrowia lipolytica Cultivation on Tuna Head Protein Hydrolysate

Ngoc Thi Minh Ta,

Nguyen Hong Ngan,

Pham Thi Lan

2023

Curr. Appl. Sci. Technol.

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“…The addition of this vitamin did not alter the lipid accumulation and growth profile of C. oleaginosus, it has been shown that thiamine metabolism has a crucial role in the lipid accumulation of Y. lipolytica by characterization of thiamine-auxotrophic strain (Walker et al, 2020). On the other hand, the lipid content of Y. lipolytica did not change by supplementing thiamine with a glycerol-based minimal medium (Das et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Model-driven engineering ofCutaneotrichosporon oleaginosusATCC 20509 for improved microbial oil production

Duman-Özdamar,

Julsing,

Verbokkem

et al. 2024

Preprint

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Consumption of plant-based oils, especially palm oil, is increasing at an alarming rate. This boosted demand for palm oil has drastic effects on the ecosystem as its production is not sustainable.C. oleaginosusis an oleaginous yeast with great potential as a source for microbial-based oil production which is a sustainable alternative to palm oil. However, microbial processes are not yet economically feasible to replace palm oil, unto a large extent due to limited lipid accumulation in the microbe, which limits titers and productivity. Therefore, obtaining enhanced lipid accumulation is essential to render this process commercially viable. Herein we deployed a systematic, iterative Design-Build-Test-Learn (DBTL) approach to establishC. oleaginosusas an efficient fatty acid production platform. In the design step, we identified genes and medium supplements that improved lipid content. To this end, we compared its transcriptional landscape in conditions with high and low amounts of lipid production. A metabolic map was reconstructed and integrated with the expression data. Finally, the genome-scale metabolic model ofC. oleaginosuswas used to explore metabolism under maximal growth and maximal production conditions. The combination of these four analyses led to the selection of four overexpression targets (ATP-citrate lyase (ACL1), acetyl-CoA carboxylase (ACC), threonine synthase (TS), and hydroxymethylglutaryl-CoA synthase (HMGS)) and five media supplements (biotin, thiamine, threonine, serine, and aspartate). We established an electroporation-based co-transformation method to implement selected genetic interventions. These findings were experimentally validated in the build and test steps of the DBTL approach by adding supplements into the medium and overexpressing the identified genes. Characterization of ACL, ACC, and TS at various C/N ratios, and the addition of medium supplements provided up to 56% (w/w) lipid content, and a 2.5-fold increase in total lipid in the glycerol and urea-based defined medium. In the learn step, quadratic models identified the optimum C/N ratios shifted towards around C/N240. These results firmly confirmC. oleaginousas a sustainable alternative to replace palm as an oil source.

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Unveiling the anticancer potentiality of single cell oils produced by marine oleaginous Paradendryphiella sp. under optimized economic growth conditions

El-Shall,

Abu‑Serie,

Abu-Elreesh

et al. 2023

Sci Rep

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Bioprospecting about new marine oleaginous fungi that produce advantageous bioproducts in a green sustainable process is the key of blue bioeconomy. Herein, the marine Paradendryphiella sp. was utilized for single cell oils (SCOs) production economically, via central composite design, the lipid content enhanced 2.2-fold by 5.5 g/L lipid yeild on seawater-based media supplemented with molasses concentration 50 g/L, yeast extract, 2.25 g/L at initial pH value (5.3) and 8 days of static incubation. Subsequently, the fatty acid methyl esters profiles of SCOs produced on optimized media under different abiotic conditions were determined; signifying qualitative and quantitative variations. Interestingly, the psychrophilic-prolonged incubation increased the unsaturation level of fatty acids to 59.34%, while ω-6 and ω-3 contents representing 23.53% and 0.67% respectively. Remarkably, it exhibited the highest EC100 dose by 677.03 µg/mL on normal human lung fibroblast Wi-38 cells. Meanwhile, it showed the highest inhibiting proliferation potential on cancer cell lines of A549, MDA-MB 231 and HepG-2 cells by 372.37, 417.48 and 365.00 µg/mL, respectively. Besides, it elevated the oxidative stress, the expression of key apoptotic genes and suppressed the expression of key oncogenes (NF-κB, BCL2 and cyclin D); implying its promising efficacy in cancer treatment as adjuvant drug. This study denoted the lipogenesis capacity of Paradendryphiella sp. under acidic/alkaline and psychrophilic/mesophilic conditions. Hereby attaining efficient and economic process under seasonal variation with different Egyptian marine sources to fill the gap of freshwater crisis and simultaneously preserve energy.

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Development of nutrient media to increase the accumulation of lipids without genetic modification of a lipogenic microorganism

Cited by 5 publications

References 19 publications

Investigation of the Yeast Yarrowia lipolytica Cultivation on Tuna Head Protein Hydrolysate

Investigation of the Yeast Yarrowia lipolytica Cultivation on Tuna Head Protein Hydrolysate

Model-driven engineering ofCutaneotrichosporon oleaginosusATCC 20509 for improved microbial oil production

Unveiling the anticancer potentiality of single cell oils produced by marine oleaginous Paradendryphiella sp. under optimized economic growth conditions

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