Oleaginous Yeasts form Uruguay and Antarctica as Renewable Raw Material for Biodiesel Production

Pereyra, Virginia

doi:10.11648/j.ajbio.20140206.20

Cited by 15 publications

(3 citation statements)

References 24 publications

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“…The microscope analysis of the C. tropicalis CMGB114 cells grown during 72 h on BS-Gly showed large vacuoles and lipid droplets which stained blue with Sudan Black B As shown by [10] lipid synthesis in Cryptococcus curvatus and Yarrowia lipolytica was induced by using minimal media supplemented with glycerol as sole carbon source or mixed with industrial lipids. Glycerol and ammonium salts were also described as inducing lipid synthesis in yeast strains isolated from soil [37]. Moreover, C. freyschussii accumulated in short time (30 h), lipids with similar composition as plant oil, when grown on same concentrations of glycerol (40 g/L) and yeast extract (3 g/ L) as those used during our experiments [38].…”

Section: Lipid Synthesis and Correlation With Biosurfactant Productionmentioning

confidence: 54%

Studies on the Correlation Between Biosurfactant and Lipid Synthesis in Candida tropicalis CMGB114 Using Hydrocarbons and Vegetable Oil Wastes

Csutak¹,

Corbu²,

Vassu³

2017

Rev. Chim.

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During past decades there is an augmented interest regarding the possibility of biosurfactant and lipid production using microorganisms able to assimilate various polluting substrates represented by hydrocarbons and industrial or household wastes such as glycerol and fried vegetable oil. The yeast strain Candida tropicalis CMGB114 produced hhigh rates of biosurfactants when grown on yeast extract and peptone supplemented with 1% petroleum, fried sunflower oil or n-hexadecane, respectively, on yeast extract and NH4NO3 with 40 g/L glycerol. Proliferating lipid bodies were observed in the cells from all the tested culture media, indicating an active lipogenesis and a correlation between biosurfactant and lipid synthesis. Future research will focus on developing strategies for practical applications of C. tropicalis CMGB114 in bioremediation and biofuel production.

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Section: Lipid Synthesis and Correlation With Biosurfactant Productionmentioning

confidence: 54%

Studies on the Correlation Between Biosurfactant and Lipid Synthesis in Candida tropicalis CMGB114 Using Hydrocarbons and Vegetable Oil Wastes

Csutak¹,

Corbu²,

Vassu³

2017

Rev. Chim.

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“…For example, the oleaginous yeast Yarrowia lipolytica YB-392 has been reported to accumulate up to 40% of lipids [mainly composed by oleic acid (C18:1), palmitic acid (C16:0) and linoleic acid (C18:2)], when cultivated in medium with a carbon to nitrogen (C:N) ratio of 100 (Liu et al, 2020). Similarly, L. scottii was previously reported to accumulate 32% of lipids when cultivated in glycerol medium with a C:N ratio of 57 (Pereyra et al, 2014). In fact, the carbon and nitrogen sources as well as their ratio, and the oxygen supply, are important factors affecting the accumulation of lipids in yeasts.…”

Section: Lipids Accumulation By Leucosporidium Scottiimentioning

confidence: 98%

“…This yeast has been isolated from Polar Regions or temperate regions during cold weather seasons, and from Antarctic habitats of seawater, soils, mosses and saline lakes (Summerbell, 1983;Sampaio, 2011). L. scottii has been reported to have ability to metabolize alternative carbon sources such as xylose and glycerol, being able to produce lipases and coenzymes Q9 and Q10 (Duarte et al, 2013) as well as to accumulate lipids (Pereyra et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

L-asparaginase Production by Leucosporidium scottii in a Bench-Scale Bioreactor With Co-production of Lipids

Moguel

Yamakawa

Pessoa

et al. 2020

Front. Bioeng. Biotechnol.

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L-asparaginase (ASNase) is a therapeutical enzyme used for treatment of acute lymphoblastic leukemia. ASNase products available in the market are produced by bacteria and usually present allergic response and important toxicity effects to the patients. Production of ASNase by yeasts could be an alternative to overcome these problems since yeasts have better compatibility with the human system. Recently, it was found that Leucosporidium scottii, a psychrotolerant yeast, produces ASNase. In order to advance the production of ASNase by this yeast, the present study aimed to select suitable process conditions able to maximize the production of this enzyme in a bench-scale bioreactor. Additionally, the accumulation of lipids during the enzyme production process was also determined and quantified. Experiments were carried out with the aim of selecting the most appropriate conditions of initial cell concentration (1.0, 3.5, and 5.6 g L–1), carbon source (sucrose and glycerol, individually or in mixture) and oxygen transfer rate (kLa in the range of 1.42–123 h–1) to be used on the production of ASNase by this yeast. Results revealed that the enzyme production increased when using an initial cell concentration of 5.6 g L–1, mixture of sucrose and glycerol as carbon source, and kLa of 91.72 h–1. Under these conditions, the enzyme productivity was maximized, reaching 35.11 U L–1 h–1, which is already suitable for the development of scale-up studies. Additionally, accumulation of lipids was observed in all the cultivations, corresponding to 2–7 g L–1 (32–40% of the cell dry mass), with oleic acid (C18:1) being the predominant compound (50.15%). Since the L-asparaginase biopharmaceuticals on the market are highly priced, the co-production of lipids as a secondary high-value product during the ASNase production, as observed in the present study, is an interesting finding that opens up perspectives to increase the economic feasibility of the enzyme production process.

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Yeast Activities Involved in Carbon and Nitrogen Cycles in Antarctica

Vero

Garmendia

Martínez-Silveira

et al. 2019

Springer Polar Sciences

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Oleaginous Yeasts form Uruguay and Antarctica as Renewable Raw Material for Biodiesel Production

Cited by 15 publications

References 24 publications

Studies on the Correlation Between Biosurfactant and Lipid Synthesis in Candida tropicalis CMGB114 Using Hydrocarbons and Vegetable Oil Wastes

Studies on the Correlation Between Biosurfactant and Lipid Synthesis in Candida tropicalis CMGB114 Using Hydrocarbons and Vegetable Oil Wastes

L-asparaginase Production by Leucosporidium scottii in a Bench-Scale Bioreactor With Co-production of Lipids

Yeast Activities Involved in Carbon and Nitrogen Cycles in Antarctica

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