Production of slime by coagulase-negative staphylococci (CNS) isolated from clinical and subclinical mastitis in cows

Abstract

The aim of the study was to evaluate the slime-producing ability of coagulase-negative staphylococci (CNS) isolated from clinical and subclinical mastitis in cows. The study was carried out on 100 isolates of CNS obtained from milk of 86 cows from farms located in the Lublin region (Poland). Slime-producing ability was observed in over half of coagulase-negative staphylococci (54.0% of isolated CNS), including 19 isolates of methicillin-resistant staphylococci (95.5% of all MRCNS). Of 22 isolates of CNS responsible for the clinical form of mastitis, 20 isolates (90.9%) produced slime: S. xylosus (7 isolates), S. haemolyticus (6 isolates), S. chromogenes (4 isolates), and S. sciuri (3 isolates), including 9 isolates of MRCNS (45.0%). The remaining 34 isolates of CNS (43.6%) with the ability to produce this exopolysaccharide were isolated from the milk of cows with subclinical form of mastitis: S. xylosus (12 isolates), S. sciuri (9 isolates), S. chromogenes (6 isolates), S. haemolyticus (3 isolates), S. warneri (3 isolates) and S. saprophyticus (1 isolate), including 10 isolates of MRCNS (12.8%).

The resident pathobiont Staphylococcus xylosus in Nfkbiz-deficient skin accelerates spontaneous skin inflammation

“…We found a correlation between the expansion of S. xylosus and the severity of inflammation in Nfkbiz −/− mice, a finding that is supported by a recent study describing the dominant expansion of S. aureus among patients with more severe AD 34 . Moreover, the fact that antibiotics specific to S. xylosus resulted in the complete suppression of dermatitis 35, 36 supports the notion that the dysbiosis provoked by the predominance of S. xylosus is closely related to skin inflammation.…”

Coagulase Negative Staphylococci as an emerging cause of bovine mastitis: prevalence, antimicrobial resistance and biofilm formation

“…At the species level; 70% of S. epidermidis, 60% of S. xylosus, 58.3% of S.chromogenes, 57.1% of S. haemolyticus, 50% of S. caprae and 33.3% of both S. hominis and S. sicuri were positive for phenotypic biofilm formation on CRA. Slime-producing ability was observed by in 54% of recovered CNS in the study by Bochniarz et al, (2014). Osman et al (2015) reported that 70.2 % of recovered CNS species were positive for their ability to produce slime and those with slime-positive strains which appeared as black colonies.…”

“…Slime production and the ability to adhere to surfaces, facilitating the formation of a biofilm, is one of the important factors responsible for the CNS pathogenicity. It's also one of the most important elements in the intramammary survival of such organisms (Bochniarz et al, 2014).…”

Carvacrol Essential Oil: A Natural Antibiotic against Zoonotic Multidrug-Resistant Staphylococcus Species Isolated from Diseased Livestock and Humans

“…In this study, the isolates that were biofilm producers were consistent with the results found by El-Seedy et al [ 28 ]. Moreover, Bochniarz et al [ 12 ] observed a slime-producing ability in 54% of the recovered Staphylococci while higher values were recorded. [ 31 , 32 , 33 ].…”

“…Additionally, they were discovered to be capable of producing proteolytic enzymes, exotoxins and haemolysins that aid in the uptake of iron such as staphylococcal protein A (SpA), the staphylococcal binder of immunoglobulin (Sbi), adenosine synthase A (AdsA), PVL (associated with staphylococcal skin and pulmonary infections), toxic shock syndrome toxin (TSST-1) and enterotoxins which lead to staphylococcal food poisoning. [7,12]. Apart from additional virulence factors, Staphylococci are protected from both the local and systemic host immunity [13].…”