Production of Single Cell Protein (SCP) from Vinasse

Martínez, Ernesto Acosta; Santos, Jéssica Ferreira dos; Araújo, Geiza Suzart; Souza, Sílvia Maria Almeida de; Rodrigues, Rita de Cássia Lacerda Brambilla; Canettieri, Eliana Vieira

doi:10.1007/978-3-319-90379-8_10

Cited by 5 publications

(7 citation statements)

References 47 publications

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“…3 As a result of its strong respiratory capacity, this strain possesses excellent intracellular protein synthesis ability, with protein content regularly exceeding 50% of the cell's dry weight, 4 surpassing most other yeast species. Consequently, C. jadinii has been implemented in the production of single-cell protein 4 and an array of industrial enzymes, including xylanase, 5 phospholipase B, 6 phytase, 7 monellin, 8 and glutathione, 9 among others. Furthermore, C. jadinii demonstrates superior properties for producing valuable compounds.…”

Section: ■ Introductionmentioning

confidence: 99%

“…C. jadinii displays the characteristics of a "Crabtree-negative" yeast, which tends to direct sugar metabolism to respiratory metabolism and biomass accumulation under aerobic conditions. 3 As a result of its strong respiratory capacity, this strain possesses excellent intracellular protein synthesis ability, with protein content regularly exceeding 50% of the cell's dry weight, 4 surpassing most other yeast species. Consequently, C. jadinii has been implemented in the production of single-cell protein 4 and an array of industrial enzymes, including xylanase, 5 phospholipase B, 6 phytase, 7 monellin, 8 and glutathione, 9 among others.…”

Section: ■ Introductionmentioning

confidence: 99%

“…jadinii displays the characteristics of a “Crabtree-negative” yeast, which tends to direct sugar metabolism to respiratory metabolism and biomass accumulation under aerobic conditions . As a result of its strong respiratory capacity, this strain possesses excellent intracellular protein synthesis ability, with protein content regularly exceeding 50% of the cell’s dry weight, surpassing most other yeast species. Consequently, C.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, C. jadinii has been implemented in the production of single-cell protein and an array of industrial enzymes, including xylanase, phospholipase B, phytase, monellin, and glutathione, among others. Furthermore, C.…”

Section: Introductionmentioning

confidence: 99%

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Development of CRISPR/Cas9-Based Genome Editing Tools for Polyploid Yeast Cyberlindnera jadinii and Its Application in Engineering Heterologous Steroid-Producing Strains

Gu,

Zhang,

Fan

et al. 2023

ACS Synth. Biol.

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In this study, a suite of efficient CRISPR/Cas9 tools was developed to overcome the genetic manipulation challenges posed by the polyploid genome of industrial yeast Cyberlindnera jadinii. The developed CRISPR/Cas9 system can achieve a 100% single-gene knockdown efficiency in strain NBRC0988. Moreover, the integration of a single exogenous gene into the target locus using a 50 bp homology arm achieved near-100% efficiency. The efficiency of simultaneous integration of three genes into the chromosome is strongly influenced by the length of the homology arm, with the highest integration efficiency of 62.5% obtained when selecting a homology arm of about 500 bp. By utilizing the CRISPR/Cas system, this study demonstrated the potential of C. jadinii in producing heterologous sterols. Through shake-flask fermentation, the engineered strains produced 92.1 and 81.8 mg/L of campesterol and cholesterol, respectively. Furthermore, the production levels of these two sterols were further enhanced through high-cell-density fed-batch fermentation in a 5 L bioreactor. The highest titer of campesterol reached 807 mg/L [biomass OD 600 = 294, productivity of 6.73 mg/(L•h)]. The titer of cholesterol reached 1.52 g/L [biomass OD 600 = 380, productivity of 9.06 mg/(L• h)], marking the first gram-scale production of steroidal compounds in C. jadinii.

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Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of CRISPR/Cas9-Based Genome Editing Tools for Polyploid Yeast Cyberlindnera jadinii and Its Application in Engineering Heterologous Steroid-Producing Strains

Gu,

Zhang,

Fan

et al. 2023

ACS Synth. Biol.

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“…The yeast C. jadinii is able to produce valuable bioproducts being an attractive source of biomass enriched in protein and vitamins. The richness of protein content, around 50% of dry cell weight, and amino acid diversity turn its biomass ideal as a source of protein supplement for animal feed and human consumption [ 4 ]. The high degree of tolerance to environmental changes occurring during fermentation turn C. jadinii an alternative to other established cell factory systems [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Expanding the Knowledge on the Skillful Yeast Cyberlindnera jadinii

Sousa-Silva

Vieira

Soares

et al. 2021

JoF

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Cyberlindnera jadinii is widely used as a source of single-cell protein and is known for its ability to synthesize a great variety of valuable compounds for the food and pharmaceutical industries. Its capacity to produce compounds such as food additives, supplements, and organic acids, among other fine chemicals, has turned it into an attractive microorganism in the biotechnology field. In this review, we performed a robust phylogenetic analysis using the core proteome of C. jadinii and other fungal species, from Asco- to Basidiomycota, to elucidate the evolutionary roots of this species. In addition, we report the evolution of this species nomenclature over-time and the existence of a teleomorph (C. jadinii) and anamorph state (Candida utilis) and summarize the current nomenclature of most common strains. Finally, we highlight relevant traits of its physiology, the solute membrane transporters so far characterized, as well as the molecular tools currently available for its genomic manipulation. The emerging applications of this yeast reinforce its potential in the white biotechnology sector. Nonetheless, it is necessary to expand the knowledge on its metabolism, regulatory networks, and transport mechanisms, as well as to develop more robust genetic manipulation systems and synthetic biology tools to promote the full exploitation of C. jadinii.

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