Production of rosmarinic acid in high density perfusion cultures ofAnchusa officinalis using a high sugar medium

Su, Wanting; Humphrey, Arthur E.

doi:10.1007/bf01022597

Cited by 31 publications

(8 citation statements)

References 3 publications

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“…Results in this research show that CA in leaves (which are photosynthetic tissue) compared with those nonphotosynthetic tissues, such as callus. The concentration of growth regulators often affected [16,17]. In the present study unorganized callus of rosemary could synthesize CAR, but at low concentrations.…”

Section: Rt (Min)mentioning

confidence: 45%

Effect of Some Biotic and Abiotic Elicitors on Phenolic Acids and Diterpenes Production from Rosemary (Rosmarinus officinalis L.) Leaf and Callus Analyzed by High Performance Liquid Chromatography (Hplc)

Rashid¹,

Ibrahim²,

Hamza

2011

JNUS

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The yield of phenolic acids; rosmarinic acid (RA), caffeic acid (CAF) and phenolic diterpenes; carnosic acid (CA), carnosol (CAR), rosmanol (ROL) from rosemary (Rosmarinus officinalis L.) was investigated. Aqueous extraction was conducted for leaf and callus tissues, the chemical analysis for extracts was carried out using high performance liquid chromatography (HPLC). Data showed that RA and CAF production was 4.5, 2.7 µg/ml respectively. CA and CAR production reached 3.3, 2.8 µg/ml when the fungal elicitor; Fusarium oxysporum was added at the concentration 2.0 mg/l (2×10 4 CFU/ml), except ROL which was found at high levels (4.3, 4.2 and 4.6 µg/ml) only from leaf extracts, untreated callus and when callus treated with 0.4 mg/l of CaCl 2 respectively.

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Section: Rt (Min)mentioning

confidence: 45%

Effect of Some Biotic and Abiotic Elicitors on Phenolic Acids and Diterpenes Production from Rosemary (Rosmarinus officinalis L.) Leaf and Callus Analyzed by High Performance Liquid Chromatography (Hplc)

Rashid¹,

Ibrahim²,

Hamza

2011

JNUS

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“…It has been reported that elicitors influenced RA biosynthesis in numerous plant in vitro cultures and that RA may be accumulated in non-organ and organ cultures in higher concentration than in the intact plants (Petersen and Simmonds 2003;Matkowski 2008). Production of bioactive compounds in a number of plant cultures was affected by sucrose concentration (Su and Humphrey 1990;Gertlowski and Petersen 1993) or by jasmonates treatment (Zhao et al 2005). This is the first report on the synthesis of RA by an E. planum cell culture and to the best of our knowledge in Apiaceae family.…”

Section: Resultsmentioning

confidence: 68%

Accumulation of rosmarinic, chlorogenic and caffeic acids in in vitro cultures of Eryngium planum L.

Kikowska

Budzianowski

Krawczyk³

et al. 2012

Acta Physiol Plant

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Eryngium planum L. cell and organ cultures were maintained on Murashige and Skoog media (MS), supplemented with exogenous hormones of different types and various concentrations for high biomass growth. The callus and cell suspension cultures were treated with increased sucrose concentration and/or elicited by methyl jasmonate for the enhancement of selected phenolic acids accumulation. Three phenolic acids, rosmarinic acid (RA), chlorogenic acid (CGA) and caffeic acid (CA), were detected by HPLC-DAD in those cultures. The sum of their content in the dry material was found to be higher in the shoot culture (3.95 mg g -1 ), root culture (7.05 mg g -1 ), callus (6.20 mg g -1 ) and cell suspension (2.04 mg g -1 ) than in the leaves (1.87 mg g -1 ) and roots (0.76 mg g -1 ) of intact plants. The major compound of in vitro cultures was always rosmarinic acid. The content of RA could be increased approximately threefold (16.24 mg g -1 ) in the callus culture and approximately twofold (3.91 mg g -1 ) in the cell suspension culture by elicitation with 100 lM methyl jasmonate (MeJA). The higher concentration of sucrose (S) in the medium (5, 6 %) led to over a twofold increase of CGA content in the callus culture (2.54 mg g -1 ).The three mentioned phenolic acids have been found in E. planum undifferentiated and differentiated in vitro cultures for the first time.

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“…This suggests that RA productivity (g of RA/reactor volumemtime) can be increased at higher sucrose concentrations because of smaller cell size. The advantage of smaller cell size in high cell density cultures was mentioned by Su and Humphrey (1990). For C. blumei, the upper limit of sucrose concentration seems to be approximately 70 g/L considering plasmolysis a t 75 g/L sucrose (Ulbrich et al, 1985).…”

Section: Resultsmentioning

confidence: 99%

Characteristics of Batch Suspension Cultures of Preconditioned Coleus blumei Cells: Sucrose Effect

Martínez

Park

1993

Biotechnology Progress

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Batch suspension cultures of Coleus blumei cells preconditioned with 0.1% dimethyl sulfoxide (DMSO) maintained viability at above 85 % during a 14‐day culturing period for initial sucrose concentrations of 30–70 g/L. At 60 g/L sucrose, cells grew rapidly with a doubling time of 10.7 h, and dry cell mass reached a maximum of 15.6 g/L. Broad optima of cell yield (0.46–0.48 g of cell/g of sucrose), rosmarinic acid (RA) yield (3.1–3.5 g of RA/100 g of sucrose), and rosmarinic acid production (1.0–1.1 g of RA/L) were observed at 40–60 g/L sucrose concentration. Sucrose in the medium was hydrolyzed to glucose and fructose by extracellular and/or cell‐bound invertase, and glucose was taken up by the cells at a higher rate than fructose. Even at sucrose concentrations of 40–70 g/L, sucrose consumption over 14 days was limited to 33 g/L.

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Production of rosmarinic acid in high density perfusion cultures ofAnchusa officinalis using a high sugar medium

Cited by 31 publications

References 3 publications

Effect of Some Biotic and Abiotic Elicitors on Phenolic Acids and Diterpenes Production from Rosemary (Rosmarinus officinalis L.) Leaf and Callus Analyzed by High Performance Liquid Chromatography (Hplc)

Effect of Some Biotic and Abiotic Elicitors on Phenolic Acids and Diterpenes Production from Rosemary (Rosmarinus officinalis L.) Leaf and Callus Analyzed by High Performance Liquid Chromatography (Hplc)

Accumulation of rosmarinic, chlorogenic and caffeic acids in in vitro cultures of Eryngium planum L.

Characteristics of Batch Suspension Cultures of Preconditioned Coleus blumei Cells: Sucrose Effect

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