Accumulation of rosmarinic, chlorogenic and caffeic acids in in vitro cultures of Eryngium planum L.

Abstract

Eryngium planum L. cell and organ cultures were maintained on Murashige and Skoog media (MS), supplemented with exogenous hormones of different types and various concentrations for high biomass growth. The callus and cell suspension cultures were treated with increased sucrose concentration and/or elicited by methyl jasmonate for the enhancement of selected phenolic acids accumulation. Three phenolic acids, rosmarinic acid (RA), chlorogenic acid (CGA) and caffeic acid (CA), were detected by HPLC-DAD in those cultures. The sum of their content in the dry material was found to be higher in the shoot culture (3.95 mg g -1 ), root culture (7.05 mg g -1 ), callus (6.20 mg g -1 ) and cell suspension (2.04 mg g -1 ) than in the leaves (1.87 mg g -1 ) and roots (0.76 mg g -1 ) of intact plants. The major compound of in vitro cultures was always rosmarinic acid. The content of RA could be increased approximately threefold (16.24 mg g -1 ) in the callus culture and approximately twofold (3.91 mg g -1 ) in the cell suspension culture by elicitation with 100 lM methyl jasmonate (MeJA). The higher concentration of sucrose (S) in the medium (5, 6 %) led to over a twofold increase of CGA content in the callus culture (2.54 mg g -1 ).The three mentioned phenolic acids have been found in E. planum undifferentiated and differentiated in vitro cultures for the first time.

Composition, antimicrobial and antioxidant activity of the extracts of Eryngium palmatum Pančić and Vis. (Apiaceae)

“…The content of chlorogenic acid in the roots of E. palmatum (1.6±0.0 mg g -1 ) was higher than the previously reported content of chlorogenic acid in the roots of E. planum (0.19±0.00 mg g -1 ) [21]. The roots of different species of the genus Eryngium contain rosmarinic acid in the range 0.4-272.0 mg g -1 [5,21]. In the methanol extract of roots of E. palmatum a moderate quantity of rosmarinic acid was determined.…”

“…The presence of chlorogenic acid was previously reported in the leaves and flowers of E. bourgatii [6], leaves of E. foetidum L. [3] and leaves and roots of E. planum L. [21]. The content of chlorogenic acid in the roots of E. palmatum (1.6±0.0 mg g -1 ) was higher than the previously reported content of chlorogenic acid in the roots of E. planum (0.19±0.00 mg g -1 ) [21]. The roots of different species of the genus Eryngium contain rosmarinic acid in the range 0.4-272.0 mg g -1 [5,21].…”

High production of bioactive depsides in shoot and callus cultures of Aronia arbutifolia and Aronia × prunifolia

“…Results from other research centres have also proved an interesting amount of DE in in vitro cultures of species representing various taxa, other than the families Lamiaceae and Boraginaceae, where the most prominent metabolite is rosmarinic acid . For example, significant amounts of DE-chlorogenic acid and rosmarinic acid-have been proven in cultures of Eryngium planum (Apiaceae) (Kikowska et al 2012) and of chlorogenic acid in cultures of Hypericum perforatum (Hypericaceae) (Cui et al 2010). In addition, our currently presented research results prove that in vitro cultures of the species of the family Rosaceae are also a rich source of DE.…”

daptation of the in vitro Culture of Origanum majorana L. For Production of Phenolic Acids

“…The sterile seeds were transferred on MS medium; 4.4 g/L containing 3% sucrose in 200 mL glass jars; 30 mL/ jar [28,29] and then incubated at 25C  2 with 16 hr. photoperiod [30] .…”