Production of rice straw hydrolysis enzymes by the fungi Trichoderma reesei and Humicola insolens using rice straw as a carbon source

Abstract

Rice straw was evaluated as a carbon source for the fungi, Trichoderma reesei and Humicola insolens, to produce enzymes for rice straw hydrolysis. The enzyme activity of T. reesei and H. insolens cultivated in medium containing non-treated rice straw were almost equivalent to the enzyme of T. reesei cultivated in Avicel medium, a form of refined cellulose. The enzyme activity of T. reesei cultivated in medium containing NHOH-treated rice straw was 4-fold higher than enzyme from cultures grown in Avicel medium. In contrast, H. insolens enzyme from cultures grown in NHOH-treated rice straw had significantly lower activity compared with non-treated rice straw or Avicel. The combined use of T. reesei and H. insolens enzymes resulted in a significant synergistic enhancement in enzymatic activity. Our data suggest that rice straw is a promising low-cost carbon source for fungal enzyme production for rice straw hydrolysis.

Rice straw as a feedstock for biofuels: Availability, recalcitrance, and chemical properties

“…HSQC is the most frequently collected 2D NMR spectrum that is used not only for structural identification but also for estimation of the relative abundance of inter‐unit linkages as well as S/G ratios in lignin. Although this technique is not quantitative but it has been widely employed to offer relative comparisons of inter‐unit linkage levels in biomass lignins, such as alfalfa, eucalyptus, poplar, wheat straw, and rice straw …”

“…Although this technique is not quantitative but it has been widely employed to off er relative comparisons of inter-unit linkage levels in biomass lignins, such as alfalfa, eucalyptus, poplar, wheat straw, and rice straw. 65,[75][76][77][78] Lignin is primarily composed of three types of monolignols/hydroxycinnamyl alcohols (p-coumaryl, coniferyl, and sinapyl alcohols) linked with each other by diff erent types of ether and carbon-carbon bonds like β-O-4, 4-O-5, β-β, β-1 and β-5 to make phenylpropanoid units such as p-hydroxyphenyl (H), guaiacyl (G) and syringyl (S). Among these, the β-O-4 linkage is the most predominant ether bond (about 40-60%) in rice straw lignin.…”

Effect of agro-industrial residues mixtures on the production of endoglucanase by Aspergillus niger in solid state fermentation

“…Better inducing capability of sugarcane bagasse could be attributed to the high content of cellulose of this substrate (Table 1). In contrast, Kogo et al (2017) reported that rice straw was a viable carbon source for fungal production of cellulases. Figure 1.…”

Xylanase production from a new strain of Aspergillus terreus S9 and its application for saccharification of rice straw using combinatorial approach

“…Rice straw is one of the most abundant agricultural wastes in tropical countries (e.g., India, China) that can be exploited for production of ethanol-biofuel and other industrial products [9,16,17]. The cellulosic and hemicellulosic contents of rice straw can be enzymatically hydrolyzed only after appropriate pretreatments [2,9].…”

“…The cellulosic and hemicellulosic contents of rice straw can be enzymatically hydrolyzed only after appropriate pretreatments [2,9]. However, unavailability of suitable pretreatment regime and/or ace saccharifying enzymes pose big challenge for bioconversion of rice straw polysaccharides into simple sugars for microbial fermentation [1,9,17].…”