Effect of agro-industrial residues mixtures on the production of endoglucanase by Aspergillus niger in solid state fermentation

Abstract

The low-cost production of cellulolytic complexes that present high action at mild conditions is one of the major bottlenecks for the economic viability of the production of cellulosic ethanol. The influence of agro-industrial residues was assessed to enhance endoglucanase production by Aspergillus niger 426 grown in solid state fermentation. The highest percentage of lignin degradation was found on soybean hulls (56%) followed by sugarcane bagasse (36%) and rice straw (8.5%). The cellulose degradation, around 90%, was observed on soybean hulls and sugarcane bagasse, but only 50% on rice straw, and maximum production of endoglucanase (112.34 ± 0.984 U mL-1) was observed for soybean hulls. The best Experimental Mixture Design condition was under cultivation of 2.5 g of sugarcane bagasse, 2.3 g of rice straw and 5.2 g of soybean hulls, leading to a maximum activity of 138.92 ± 0.02 U mL-1. The statistical methodology enabled an increase of over 20% in the production of endoglucanase using agro-industrial waste. These data demonstrate that A. niger 426 is a potential source of cellulases which can be obtained by solid state fermentation using agro-industrial waste.

Amylase potential of filamentous fungi isolated from sweet potato pulp

“…The Aspergillus niger fungus crude extract was seen to be efficient in the hydrolysis of sweet potato starch (Pereira et al, 2017), simultaneous hydrolysis with fermentation in potato (Izmirliogli & Demirci, 2016), and hydrolysis and simultaneous fermentation in agro-industrial waste (Oliveira et al, 2020). By using an enzymatic cocktail produced with Penicillium sp., great efficiency was observed in the hydrolysis of cassava flour (Hai-Juan et al, 2010).…”