Production of PUFA Concentrates from Poultry and Fish Processing Waste

Patil, Dipak; Nag, Ahindra

doi:10.1007/s11746-010-1689-4

Cited by 29 publications

(24 citation statements)

References 21 publications

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“…Production of PUFA concentrate (up to 73.2% of total FA) from mackerel processing waste has been reported by using urea fractionation , and another work achieved a concentrate fraction (78.2% EPA + DHA with a yield of 11%) by using a two‐step process (concentration of free PUFA in acetone at low temperature and further urea complexation) from hilsa fish viscera . The main concern in these cases is the potential obtaining of ethyl carbamates as a product of the reaction between urea and ethanol, which has been described as hazardous .…”

Section: Resultsmentioning

confidence: 99%

Docosahexaenoic acid purification from fish processing industry by‐products

Cervera

Venegas

Ramos‐Bueno

et al. 2015

Euro J Lipid Sci & Tech

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Docosahexaenoic acid (DHA) is a well-known dietary n-3 polyunsaturated fatty acid (PUFA) found in marine fish and organisms whose beneficial effects for human health are widely reported. However, the supply of food containing DHA is currently becoming critical due to the overexploitation of fisheries worldwide. Consequently, searching for alternative sources of DHA is a relevant issue nowadays. Viscera from cultured fishes is considered a by-product by the fishery industry and therefore discarded after fish processing. In this work, viscera from farmed marine fish (Sparus aurata and Dicentrarchus labrax) and also oil extracted from such by-products have been studied to assess their suitability as DHA sources by obtaining their fatty acid profiles. Viscera oil was used as a source for DHA purification by a simple and easily scalable chromatographic method. As a result, a highly purified DHA fraction (>99.0% DHA on total fatty acids) was obtained.Practical applications: This DHA concentrate obtained from viscera oil could be potentially used for alimentary or pharmaceutical purposes due to its lower cholesterol content compared with other known marine sources and also because only safe and legally allowed food-grade solvents have been used throughout the whole process. This way, by obtaining a high added-value product, a revaluation is given to fish by-products which are usually discarded. Abbreviations: DHA, docosahexaenoic acid; EE, ethyl ester; EPA, eicosapentaenoic acid; FA, fatty acid; FAME, fatty acid methyl ester; FID, flame ionization detector; MUFA, monounsaturated fatty acids; PCB, polychlorinated biphenyls; PUFA, polyunsaturated fatty acids; SFA, saturated fatty acids 724Eur.

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Section: Resultsmentioning

confidence: 99%

Docosahexaenoic acid purification from fish processing industry by‐products

Cervera

Venegas

Ramos‐Bueno

et al. 2015

Euro J Lipid Sci & Tech

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“…Eicosapentaenoic acid (EPA C20:5 n-3) and docosahexaenoic acid (DHA, C22:6 n-3) are the most important n-3 PUFA (Sargent et al 2002;Sahena et al 2009) and marine organisms are the main natural source of them (Kim & Mendis 2006). The interest for n-3 PUFA and the decline in natural marine resources stimulated research to "find out" new sources alternative to fisheries, such as by-products originated from fish transformation industry and fisheries by-catch (Kim & Mendis 2006;Ferraro et al 2010;Patil & Nag 2011). The European sea bass (Dicentrarchus labrax L., 1758) is one of the most important fish species farmed in the Mediterranean, for which new processed products, such as ready to cook fillets and heat induced gel products are recently developed in order to realize new market opportunities (Cardoso et al 2012).…”

Section: Introductionmentioning

confidence: 99%

By-products of farmed European sea bass (Dicentrarchus labrax L.) as a potential source of n-3 PUFA

Messina

Renda²,

Barbera³

et al. 2013

Biologia

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Total by-products (TBP) obtained by filleting farmed and wild European sea bass (Dicentrarchus labrax) were analyzed to evaluate if, on the basis of the percentage yield, total lipid content and fatty acid composition, they can be considered a resource of n-3 polyunsaturated fatty acids (PUFA). Results show that TBP from intensively farmed fish (IFF) contain higher total lipid content and have a higher level of n-3 PUFA rich in eicosapentaenoic (EPA) and docosaexaenoic acid (DHA), compared to extensively farmed fish (EFF) and to wild fish (WF) (P < 0.05). This difference may suggest a way of promotion of TBP from IFF sea bass through the n-3 PUFA recovery by extraction.

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“…In previous studies, lowtemperature crystallization was commonly used to purify the polyunsaturated fatty acids. 20 We expected that a similar purification strategy would also apply to the purification of oleic acid and plan to demonstrate the effectiveness of this procedure.…”

Section: ■ Introductionmentioning

confidence: 99%

Synthesis of Oleoylethanolamide Using Lipase

Wang

2011

J. Agric. Food Chem.

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An effective process for the enzymatic synthesis of oleoylethanolamide is described in this study. The process included purification of a commercial oleic acid product and then optimization of the reaction between the purified oleic acid and ethanolamine in the presence of hexane and a lipase. Under the optimal amidation reaction conditions identified, oleoylethanolamide was obtained with 96.6% purity. The synthesis was also conducted on a large scale (50 mmol of each of the reactants), and oleoylethanolamide purity and yield after crystallization purification were 96.1 and 73.5%, respectively. Compared to the previous studies, the current method of preparing high-purity oleoylethanolamide is more effective and economically feasible. The scalability and ease for such synthesis make it possible to study the biological and nutritional functions of the cannabinoid-like oleoylethanolamide in animal or human subjects. ABSTRACT: An effective process for the enzymatic synthesis of oleoylethanolamide is described in this study. The process included purification of a commercial oleic acid product and then optimization of the reaction between the purified oleic acid and ethanolamine in the presence of hexane and a lipase. Under the optimal amidation reaction conditions identified, oleoylethanolamide was obtained with 96.6% purity. The synthesis was also conducted on a large scale (50 mmol of each of the reactants), and oleoylethanolamide purity and yield after crystallization purification were 96.1 and 73.5%, respectively. Compared to the previous studies, the current method of preparing high-purity oleoylethanolamide is more effective and economically feasible. The scalability and ease for such synthesis make it possible to study the biological and nutritional functions of the cannabinoid-like oleoylethanolamide in animal or human subjects.

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Production of PUFA Concentrates from Poultry and Fish Processing Waste

Cited by 29 publications

References 21 publications

Docosahexaenoic acid purification from fish processing industry by‐products

Docosahexaenoic acid purification from fish processing industry by‐products

By-products of farmed European sea bass (Dicentrarchus labrax L.) as a potential source of n-3 PUFA

Synthesis of Oleoylethanolamide Using Lipase

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