Production of prebiotic galactooligosaccharides from lactose by Pseudozyma tsukubaensis and Pichia kluyveri

Fai, Ana Elizabeth Cavalcante; Silva, Juliana Bueno da; Andrade, Cristiano José de; Bution, Murillo Lino; Pastore, Gláucia Maria

doi:10.1016/j.bcab.2014.04.005

Cited by 26 publications

(22 citation statements)

References 57 publications

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“…Galactooligosaccharides (GOS) are prebiotics synthesized from lactose transgalactosylation. Recent studies attribute to these oligosaccharides a number of potential health benefits [6]. Galactooligosaccharides stimulate Bifidobacterium proliferation in the colon, which suppress the activity of putrefying bacteria by antagonistic effect and reduce the formation of toxic metabolites [7,8].…”

Section: Introductionmentioning

confidence: 99%

Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs

Rentas

Pedreira²,

Perini

et al. 2020

PLoS ONE

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This study aimed to evaluate the effects of two prebiotics in different concentrations on nutrient digestibility, fermentative products and immunological variables in adult dogs. Twentyfour adult dogs were randomly divided into six blocks according to their metabolic body weights (BW 0.75); within these groups, dogs were randomized to four treatments: control without prebiotics (CO); inclusion of 0.5% prebiotic blend Yes-Golf (B1); inclusion of 1.0% galactooligosaccharide (GOS); and inclusion of 1.0% prebiotic blend Yes-Golf (B2). The experiment lasted 30 days, with 20 days adaptation and 10 days stool and blood collection. Results were analyzed for normality and means were separated by ANOVA and adjusted by the Tukey test at the significance level of 5.0%. Prebiotic supplementation had no effect on apparent digestibility coefficients (ADC), total stool production and fecal scores (p > 0.05). Prebiotics evaluated also did not alter fecal pH, nor the concentrations of ammonia, lactic acid, short chain fatty acids (SCFA) and most fecal branched chain fatty acids (BCFA) (p > 0.05). The addition of GOS decreased the concentration of iso-valeric acid (p = 0.0423). Regarding immunological variables, concentrations of fecal IgA were not influenced by the treatments. Treatments GOS and B2 increased the total number of polymorphonuclear cells, as well as the oxidative burst in relation to treatments B1 and CO (p < 0.0001). Treatment B2 improved the rate of S. aureus phagocytosis in relation to CO (p = 0.0111), and both the GOS and B2 treatments had a better index for E. coli phagocytosis than the CO treatment (p = 0.0067). In conclusion, there was indication that both prebiotics GOS and B2 at 1.0% inclusion improved the immunity of healthy dogs.

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Section: Introductionmentioning

confidence: 99%

Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs

Rentas

Pedreira²,

Perini

et al. 2020

PLoS ONE

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“…Microorganisms P. kluyveri and P. tsukubaensis were previously isolated from peach (Prunus persica) and nectarine (P. persica var. nucipersica) fruits, respectively, identified by 28S rDNA gene sequencing and phylogenetic analysis and deposited in the GenBank database with accession numbers KF268596 and KF268597, for P. kluyveri and P. tsukubaensis, respectively (Fai et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

“…Despite considerable work being performed in the last years to improve GOS synthesis process, few studies focused on the alternative to produce GOS by fermentation (Fai et al, 2014;Onishi et al, 1996;Onishi et al, 1995). Thus, the objective of this paper was to optimize the production of GOS by Pseudozyma tsukubaensis and Pichia kluyveri fermentation of lactose, using an experimental design and surface analysis methodology.…”

Section: Introductionmentioning

confidence: 98%

“…As for most reactions catalyzed by a biological entity, GOS production can be accomplished in various manners. GOS can be produced either by enzymes or by fermentation, and lactose serves as both a galactosyl donor and an acceptor to yield di-, tri-, or higher oligosaccharides (Bicas, Silva, Dionísio, & Pastore, 2010;Fai, Silva, Andrade, Bution, & Pastore, 2014;Goslin et al, 2010;Kim, Lee, & Lee, 2001). Although many microbial products are synthesized by extracellular release, it is suggested that much larger proportions of the potentially useful microbial products be retained within the cells (Panesar, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sequential optimization approach for prebiotic galactooligosaccharides synthesis by Pseudozyma tsukubaensis and Pichia kluyveri

Fai

Simiqueli

Ghiselli

et al. 2015

LWT - Food Science and Technology

Self Cite

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“…Os galactooligossacarídeos (GOS) são prebióticos de alto valor agregado, sintetizados a partir da transgalactosilação da lactose. Estudos recentes atribuem a estes oligossacarídeos uma série de potenciais benefícios à saúde (FAI & PASTORE, 2015). Seus efeitos positivos ao animal ocorrem principalmente devido ao aumento da população de Bifidobactérias no cólon e por efeito antagônico, supressão da atividade de bactérias putrefativas, reduzindo a formação de metabólitos tóxicos (NERI et al, 2009;MONTILLA et al, 2015).…”

Section: Lista De Quadrosunclassified

>b<Efeitos da adição de diferentes promotores de saúde intestinal na digestibilidade, produtos fermentativos e parâmetros imunológicos em cães>/b<

Rentas¹

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, pela orientação e ensinamentos tanto para minha formação acadêmica como pessoal, pela confiança, amizade, paciência, disponibilidade e oportunidade.  A toda equipe de funcinários, colaboradores e animais do CEPEN PET por fazerem meus dias mais felizes, amo vocês.  A Deus, por me dar saúde e força para conseguir realizar esse trabalho.  Aos meus pais, Robson e Miriam por toda a dedicação, esforço e amor para que eu pudesse realizar mais esse sonho.  Ao meu irmão, Marco Aurélio pela companhia, carinho, risadas, brigas e crescimento, sem você eu não seria a mesma.  Aos meus avós Shirley, Nelson (in memoriam), Edna e René por serem essas pessoas tão maravilhosas.  Aos meus padrinhos Isabel e Renato, e a todos os meus tios, tias e primos por sempre torcerem por mim e me ajudar quando preciso.  Aos meus melhores amigos, Renan Zala, Talita Alves, e Filipe Zanferari, e afilhada Pietra que estão sempre presentes mesmo distantes, enchendo meu coração de alegria.  Ao meu namorado, Caio Ueda Arata (Vel) por cuidar de mim nesses momentos em que nem eu mesma achava que conseguiria.  A Nozes, minha segunda família. Vocês tornam meus dias mais sorridentes, obrigada por tudo, vocês são demais: Lari, Robs, Dressete, Perini, Tobs, Rafa e Cati.

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Production of prebiotic galactooligosaccharides from lactose by Pseudozyma tsukubaensis and Pichia kluyveri

Cited by 26 publications

References 57 publications

Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs

Galactoligosaccharide and a prebiotic blend improve colonic health and immunity of adult dogs

Sequential optimization approach for prebiotic galactooligosaccharides synthesis by Pseudozyma tsukubaensis and Pichia kluyveri

>b<Efeitos da adição de diferentes promotores de saúde intestinal na digestibilidade, produtos fermentativos e parâmetros imunológicos em cães>/b<

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