Production of lactic acid using a new homofermentative <scp>E</scp>nterococcus faecalis isolate

Subramanian, Mohan Raj; Talluri, Suvarna; Christopher, Lew P.

doi:10.1111/1751-7915.12133

Cited by 43 publications

(26 citation statements)

References 27 publications

(27 reference statements)

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“…The best titer in these studies was reported from a fed-batch fermentation with a cellobiose-xylose mixture, reaching 163 g·L −1 l-(+)-LA of >99% optical purity in 240 h [33]. l-(+)-LA titers of 182, 134.9, and 93 g·L −1 were achieved with E. faecalis in batch fermentations utilizing glucose, sugar molasses, and oak wood chips, respectively [34][35][36]. Overall productivities using glucose, sugar molasses, and oak wood chips were 5.0, 4.3, and 1.7 g·L −1 ·h −1 , respectively.…”

Section: Lactic Acidmentioning

confidence: 94%

Multi-Product Lactic Acid Bacteria Fermentations: A Review

Mora-Villalobos¹,

Montero-Zamora²,

Barboza

et al. 2020

Fermentation

123

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Industrial biotechnology is a continuously expanding field focused on the application of microorganisms to produce chemicals using renewable sources as substrates. Currently, an increasing interest in new versatile processes, able to utilize a variety of substrates to obtain diverse products, can be observed. A robust microbial strain is critical in the creation of such processes. Lactic acid bacteria (LAB) are used to produce a wide variety of chemicals with high commercial interest. Lactic acid (LA) is the most predominant industrial product obtained from LAB fermentations, and its production is forecasted to rise as the result of the increasing demand of polylactic acid. Hence, the creation of new ways to revalorize LA production processes is of high interest and could further enhance its economic value. Therefore, this review explores some co-products of LA fermentations, derived from LAB, with special focus on bacteriocins, lipoteichoic acid, and probiotics. Finally, a multi-product process involving LA and the other compounds of interest is proposed.Fermentation 2020, 6, 23 2 of 21 by-products, were proposed to improve industrial single-product biotechnological processes [2]. One example is the use of cellulose and hemicellulose from sugarcane bagasse (a residue obtained during the bioethanol production and usually used for energy generation) for the production of value-added chemicals [3]. Likewise, glycerol, a by-product of the biodiesel industry with low value in the market, is targeted as a molecule of interest in fermentation processes. Such multi-product processes, based on the conversion of renewable materials into biobased products, are known as biorefineries [4].The study, development, and application of robust microbial strains, able to utilize a variety of substrates and to produce a wide range of products, is considered a milestone in the development of biorefineries [4]. Lactic acid bacteria (LAB) are a diverse group with recognized potential for the development of integrated biorefineries [5]. LAB are non-sporulating, non-motile, acid-tolerant, non-respiring but aerotolerant, catalase-negative, Gram-positive cocci or rods. They are characterized by the production of lactic acid (LA) as the major end metabolic product of carbohydrate fermentation [6][7][8][9]. Given the lack of a functional respiratory system, LAB obtain energy through substrate-level phosphorylation following two metabolic pathways for hexose fermentation, i.e., homofermentative and heterofermentative. As shown in Figure 1, the first pathway is based on glycolysis with the production of mainly LA, whereas the second one, known as the pentose phosphate pathway, is characterized for the production of CO 2 and ethanol, or acetate in addition to LA [6].

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Section: Lactic Acidmentioning

confidence: 94%

Multi-Product Lactic Acid Bacteria Fermentations: A Review

Mora-Villalobos¹,

Montero-Zamora²,

Barboza

et al. 2020

Fermentation

123

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“…and Lactococcus (L.) sp. are the predominant species used for lactic acid production, but we have recently reported a high yield lactic acid producing Enterococcus isolate that is a promising candidate for metabolic engineering, and does not have the complex nutritional requirements of other LAB [6,8]. Because of their innate acid tolerance, yeasts such as Candida sp.…”

Section: Challenges and Metabolic Engineering Solutions For Lactic Acmentioning

confidence: 99%

“…The commercial production process requires the use of non-fastidious and robust microorganisms that can meet the industrial needs for high yield, productivity and titer of lactic acid of at least 80%, 2.5 g/l/h and 100 g/l, respectively [8]. Other important fermentation parameters include specific growth rate of the production organism, metabolite formation, ability to ferment both pentose and hexose sugars, no substrate or end-product inhibition, resistance to inhibitory compounds formed during pre-treatment of lignocellulosic biomass, and minimizing carbon catabolite repression [44].…”

Section: Challenge 4: Parameters For Industrial Lactic Acid Productionmentioning

confidence: 99%

Metabolic engineering as a tool for enhanced lactic acid production

Upadhyaya

DeVeaux

Christopher

2014

Trends in Biotechnology

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“…Glucose is the carbon source that has been studied the most. A recent study by Subramanian et al (2014) evaluated lactic acid production from the homofermentative strain of Enterococcus faecalis CBRD01. It obtained volumetric productivity of 3.10 g.l -1…”

Section: Discussionmentioning

confidence: 99%

Evaluation of biological production of lactic acid in a synthetic medium and in Aloe vera (L.) Burm. f. processing by-products

Gómez-Gómez

Giraldo-Estrada

Habeych³

et al. 2015

Univ. Sci.

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This study evaluated lactic acid production through batch fermentation in a bioreactor with Thermoanaerobacter sp. strain USBA-018 and a chemically defined culture medium and with hydrolyzed pressed extract of Aloe vera peel (AHE). The strain USBA-018 fermented various sugars, but its primary end-product was L-lactic acid. Factors which influenced L- lactic acid production were pH, addition of yeast extract (YE) and manganese chloride. Under the most favorable growing conditions for the production of lactic acid, yield (Yp/s) increased from 0.66 to 0.96 g/g with a productivity (Qp) of 0.62 g.l-1.h and a maximum lactic acid concentration of 178 mM at 26 hours of fermentation. When AHE was used, 93.3 mM, or 0.175 g.h/L, was obtained. These results show the potential for transformation of sugars that strain USBA-018 offers, but additional studies are needed to find out if different strategies using AHE as carbon source can produce large enough quantities of lactic acid to allow AHE to become a low-cost alternative substrate.

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Production of lactic acid using a new homofermentative Enterococcus faecalis isolate

Cited by 43 publications

References 27 publications

Multi-Product Lactic Acid Bacteria Fermentations: A Review

Multi-Product Lactic Acid Bacteria Fermentations: A Review

Metabolic engineering as a tool for enhanced lactic acid production

Evaluation of biological production of lactic acid in a synthetic medium and in Aloe vera (L.) Burm. f. processing by-products

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