Metabolic engineering as a tool for enhanced lactic acid production

Upadhyaya, Bikram; DeVeaux, Linda C.; Christopher, Lew P.

doi:10.1016/j.tibtech.2014.10.005

Cited by 79 publications

(44 citation statements)

References 61 publications

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“…Still, researchers are making efforts to further develop sustainable LA fermentation processes to achieve efficient LA production. Till date, several studies have been conducted using metabolic engineering approach for LA production with improved productivities using different carbon sources (Varman et al ; Upadhyaya et al ). Microbial LA production under acidic fermentation conditions is favourable to reduce production costs.…”

Section: Lactic Acidmentioning

confidence: 99%

Polylactic acid: synthesis and biomedical applications

2019

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Social and economic development has driven considerable scientific and engineering efforts on the discovery, development and utilization of polymers. Polylactic acid (PLA) is one of the most promising biopolymers as it can be produced from nontoxic renewable feedstock. PLA has emerged as an important polymeric material for biomedical applications on account of its properties such as biocompatibility, biodegradability, mechanical strength and process ability. Lactic acid (LA) can be obtained by fermentation of sugars derived from renewable resources such as corn and sugarcane. PLA is thus an eco‐friendly nontoxic polymer with features that permit use in the human body. Although PLA has a wide spectrum of applications, there are certain limitations such as slow degradation rate, hydrophobicity and low impact toughness associated with its use. Blending PLA with other polymers offers convenient options to improve associated properties or to generate novel PLA polymers/blends for target applications. A variety of PLA blends have been explored for various biomedical applications such as drug delivery, implants, sutures and tissue engineering. PLA and their copolymers are becoming widely used in tissue engineering for function restoration of impaired tissues due to their excellent biocompatibility and mechanical properties. The relationship between PLA material properties, manufacturing processes and development of products with desirable characteristics is described in this article. LA production, PLA synthesis and their applications in the biomedical field are also discussed.

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Section: Lactic Acidmentioning

confidence: 99%

Polylactic acid: synthesis and biomedical applications

2019

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“…The bovine gene encoding LDH has been successfully expressed in both Candida utilis and Saccharomyces cerevisiae, and the gene encoding LDH from Lb. helveticus has been expressed in Candida sonorensis [1]. Different research teams have been attempting to produce lactate from engineered yeasts genera including Saccharomyces cerevisiae [13,14,92,94,95,96,97,98], Candida spp.…”

Section: Yeastsmentioning

confidence: 99%

Lactic acid production – producing microorganisms and substrates sources-state of art

Abedi

Hashemi

2020

Heliyon

230

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Lactic acid is an organic compound produced via fermentation by different microorganisms that are able to use different carbohydrate sources. Lactic acid bacteria are the main bacteria used to produce lactic acid and among these, Lactobacillus spp. have been showing interesting fermentation capacities. The use of Bacillus spp. revealed good possibilities to reduce the fermentative costs. Interestingly, lactic acid high productivity was achieved by Corynebacterium glutamicum and E. coli, mainly after engineering genetic modification. Fungi, like Rhizopus spp. can metabolize different renewable carbon resources, with advantageously amylolytic properties to produce lactic acid. Additionally, yeasts can tolerate environmental restrictions (for example acidic conditions), being the wildtype low lactic acid producers that have been improved by genetic manipulation. Microalgae and cyanobacteria, as photosynthetic microorganisms can be an alternative lactic acid producer without carbohydrate feed costs. For lactic acid production, it is necessary to have substrates in the fermentation medium. Different carbohydrate sources can be used, from plant waste as molasses, starchy, lignocellulosic materials as agricultural and forestry residues. Dairy waste also can be used by the addition of supplementary components with a nitrogen source.

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“…Međutim, ovakva proizvodnja je ekonomski nepovoljna, zato što su rafinisani šećeri skupi, a globalna potražnja za hranom je sve veća. Takođe, MK je prilično jeftin proizvod, trenutna tržišna cena MK za prehrambenu upotrebu iznosi 1400-1600 US$ po toni [31]. Zbog toga, u novije vreme se intenzivnije razmatra proizvodnja MK iz jeftinih sirovina, kao što su otpadne sirovine iz poljoprivrede, šumarstva i industrije.…”

Section: Sporedne I Otpadne Sirovine Za Proizvodnju Mlečne Kiselineunclassified

Opportunities, perspectives and limits in lactic acid production from waste and industrial by-products

Mladenović¹,

Djukic-Vukovic²,

Pejin

et al. 2016

Hem Ind

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In line with the goals of sustainable development and environmental protection today great attention is directed towards new technologies for waste and industrial by-products utilization. Waste products represent potentially good raw material for production other valuable products, such as bioethanol, biogas, biodiesel, organic acids, enzymes, microbial biomass, etc. Since the first industrial production to the present, lactic acid has found wide application in food, cosmetic, pharmaceutical and chemical industries. In recent years, the demand for lactic acid has been increasing considerably owing to its potential use as a monomer for the production of poly-lactic acid (PLA) polymers which are biodegradable and biocompatible with wide applications. Waste and industrial by-products such are whey, molasses, stillage, waste starch and lignocellulosic materials are a good source of fermentable sugars and many other substances of great importance for the growth of microorganisms, such as proteins, minerals and vitamins. Utilization of waste products for production of lactic acid could help to reduce the total cost of lactic acid production and except the economic viability of the process offers a solution of their disposal. Fermentation process depends on chemical and physical nature of feedstocks and the lactic acid producer. This review describes the characteristics, abilities and limits of microorganisms involved in lactic acid production, as well as the characteristics and types of waste products for lactic acid production. The fermentation methods that have been recently reported to improve lactic acid production are summarized and compared. In order to improve processes and productivity, fed-batch fermentation, fermentation with immobilized cell systems and mixed cultures and opportunities of open (non-sterilized) fermentation have been investigated.

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Metabolic engineering as a tool for enhanced lactic acid production

Cited by 79 publications

References 61 publications

Polylactic acid: synthesis and biomedical applications

Polylactic acid: synthesis and biomedical applications

Lactic acid production – producing microorganisms and substrates sources-state of art

Opportunities, perspectives and limits in lactic acid production from waste and industrial by-products

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