Production of high-value bioproducts enriched with γ-linolenic acid and β-carotene by filamentous fungi Umbelopsis isabellina using solid-state fermentations

Abstract

Solid-state fermentation is a useful tool for utilizing different plant-based materials as cultivation substrates in order to produce potentially high-value fermented bioproducts. The aim of the present study was to successfully prepare various types of such bioproducts, using a zygomycetous strain Umbelopsis isabellina CCF2412. Various legume and cereal substrates were utilized effectively, while a few of them were obtained from agricultural waste, which is particularly advantageous from ecological and economic point of view. A common feature of the produced fermented materials was the increased content of different polyunsaturated fatty acids and carotenoid pigments in these bioproducts. Subsequent to the optimization of the solid-state fermentation process using cornmeal as the cultivation substrate, bioproducts enriched with γ-linolenic acid (11.45 mg γ-linolenic acid per gram of bioproduct), β-carotene (50.90 μg β-carotene per gram of bioproduct), and various microbial sterols were obtained. Appropriate n-6/n-3 acid ratio and enrichment of other microbial substances, such as the pigments and sterols mentioned above, in the fermented bioproducts widens the applicability of these bioproducts in different industries. The fermented cereal bioproducts produced in the present study from fermented wheat bran substrate were used for evaluating their application as feed for broiler chicken, and satisfactory results were obtained. Therefore, the present study creates novel opportunities for improving the quality of fermented bioproducts obtained during solid-state fermentation processes, especially for application in the feed industry.

Effect of Solid-State Fermented Wheat Bran Supplemented with Agrimony Extract on Growth Performance, Fatty Acid Profile, and Meat Quality of Broiler Chickens

“…In recent years, several studies have been published, which detail the use of filamentous fungi in SSF and its successful application in broiler nutrition. Specific examples include the use of Umbelopsis isabellina CCF2412 on cornmeal [ 7 , 10 , 11 ], Trichoderma pseudokoningii on wheat bran [ 4 ], Acremonium charticola and Rhizopus oryzae on cassava pulp [ 3 ], Cunninghamella elegans CCF 2591 on spelt brans [ 12 ], Trichoderma viride on copra meal [ 13 ], and Trichoderma virens on palm-fruit husk [ 14 ]. Čertík et al [ 15 ] observed that SSF with Cunninghamella echinulata ATHUM 4411 produces mainly gamma-linolenic acid (18:3, GLA).…”

Cultivable cellulolytic fungi isolated from the gut of Amazonian aquatic insects

“…Overall, we found that the guts of Phylloicus, Triplectides and Stenochironomus larvae analyzed in this study are colonized by Cladosporium, Gliocephalotrichum, Penicillium, Pestalotiopsis, Talaromyces Benjamin, Trichoderma and Umbelopsis fungi that have species that are traditionally used in biotechnological applications (Jalmi et al 2012;Copete-Pertuz et al 2019;Geetha et al 2019;Liuzzi et al 2019;Salazar-Cerezo et al 2019;Ge et al 2020;Slaný et al 2020).…”

Animal Fat as a Substrate for Production of n-6 Fatty Acids by Fungal Solid-State Fermentation

“…A specific fermentation process called solid-state fermentation (SSF) is a well-described process using a solid matrix and almost no water, accompanied by a high-production capacity [ 11 , 12 ]. Application of SSF with Zygomycetes fungi as a main production cell factories allow acquiring of bioproducts enriched with various long-chain unsaturated fatty acids [ 6 , 13 , 14 ] and also other specific valuable products, such as glucosamine [ 15 ], carotenoid pigments [ 6 , 16 , 17 , 18 ], coenzymes [ 19 ], etc. Furthermore, the process of SSF often leads to the increased enzyme activity (e.g.…”

“…Fungus Umbelopsis isabellina , used in this study, is a relatively well-known Zygomycetes species for application in bioremediation [ 29 , 30 ] and fermentation processes [ 16 , 17 , 31 ]. Its ability to effectively produce long-chain fatty acid and carotenoid pigments was described previously [ 16 , 31 , 32 ].…”

“…Effective production of such high value-added fermented products with no need for their fractionation predetermines it for possible direct industry usage, e.g. in the feed or food industry [ 17 , 35 ].…”