Production of High Levels of Th1 and Th2 Cytokines in Mice with Acquired Transplantation Tolerance

Holáň, Vladimı́r; Sedláčková, Kateřina; Růžičková, Michaela

doi:10.1006/cimm.1996.0287

Cited by 15 publications

(3 citation statements)

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“…Supernatants were harvested after 24 h for IL‐2; 48 h for IFN‐ γ and 72 h for IL‐4 and IL‐10. The production of cytokines in the supernatants was measured by enzyme‐linked immunosorbent assay (ELISA) using sets of cytokine‐specific capture and detection mAb purchased from PharMingen (San Diego, CA, USA) [18]. For quantification of cytokine levels, standards for IL‐2, IL‐4, IL‐10 and IFN‐ γ (Genzyme, Boston, MA, USA) were included in all ELISA determinations.…”

Section: Methodsmentioning

confidence: 99%

Corneal rat-to-mouse xenotransplantation and the effects of anti-CD4 or anti-CD8 treatment on cytokine and nitric oxide production

Pindjáková¹,

Vítová²,

Krulová³

et al. 2005

Transplant Int

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Summary Corneal xenotransplantation may be an alternative approach to overcome shortage of allografts for clinical transplantation. Orthotopic corneal rat‐to‐mouse xenotransplantation and syngeneic transplantation was performed and the effects of anti‐CD4 and anti‐CD8 treatments on corneal xenograft survival and production of cytokines, interleukin (IL)‐2, IL‐4, IL‐10, γ‐interferon (IFN‐γ) and nitric oxide (NO) were evaluated. RT‐PCR was used to determine the expression of genes for cytokines and inducible nitric oxide synthase (iNOS) in the grafts. The presence of iNOS protein in grafts was detected by immunofluorescent staining. We found that corneal xenotransplantation was associated with a strong upregulation of genes for both Th1 and Th2 cytokines and with NO production in the graft. Treatment of xenograft recipients with mAb anti‐CD4, but not anti‐CD8, resulted in a profound inhibition of IL‐2, IL‐4 and IL‐10 production, and in a significant prolongation of corneal xenograft survival. The results show that upregulation of Th2 cytokines after corneal xenotransplantation does not correlate with xenograft rejection. Rather, corneal graft rejection is associated with the expression of genes for IFN‐γ and iNOS and with NO production.

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Section: Methodsmentioning

confidence: 99%

Corneal rat-to-mouse xenotransplantation and the effects of anti-CD4 or anti-CD8 treatment on cytokine and nitric oxide production

Pindjáková¹,

Vítová²,

Krulová³

et al. 2005

Transplant Int

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“…Cell culture supernatants were harvested at various time‐points (48–96 hr) and stored at −20° until assayed. The presence of IL‐2, IL‐4, IL‐10 and IFN‐γ in the supernatants was measured by enzyme‐linked immunosorbent assay (ELISA) 15 using sets of cytokine‐specific capture and detection monoclonal antibodies (mAbs) purchased from PharMingen (San Diego, CA) and exactly according to the manufacturer's instructions. For quantification of cytokine levels, standards for IL‐2, IL‐4, IL‐10 and IFN‐γ (all purchased from Genzyme, Cambridge, MA) were included in all ELISA determinations.…”

Section: Methodsmentioning

confidence: 99%

Induction of specific transplantation immunity by oral immunization with allogeneic cells

et al. 2000

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SUMMARYOral administration of antigen has been shown to be effective for both positive and negative modulation of immune responses. In the present study we characterized changes in the reactivity of the immune system after oral immunization with allogeneic spleen cells. Mice were orally immunized for 10 consecutive days with fresh allogeneic spleen cells, and the phenotype, proliferative response, cytotoxic activity and cytokine production pro®le of recipient spleen cells were assessed 1 or 7 days after the last immunization dose. Although no signi®cant changes in the proportion of CD4 + , CD8 + or CD25 + cells were observed in the spleen of orally immunized mice, signi®cant activation of alloreactivity in spleen cells was found. Cells from orally immunized mice exhibited enhanced proliferation and cytotoxic activity after stimulation with speci®c allogeneic cells in vitro, and produced considerably higher concentrations of interferon-c (IFN-c) and signi®cantly less interleukin (IL)-4 than did cells from control mice. The production of IL-2 was essentially unchanged and that of IL-10 was only slightly increased. The systemic allosensitization induced by oral immunization was demonstrated in vivo by increased resistance to the growth of allogeneic tumours induced by subcutaneous inoculation of high doses of tumour cells. In addition, orthotopic corneal allografts in orally immunized recipients were rejected more rapidly (in a secondset manner) than in control, untreated recipients. These data show that oral immunization with allogeneic cells modulates individual components of the immune response and that speci®c transplantation immunity, rather than tolerance, is induced in the treated recipients.

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“…The presence of cytokines in supernatants was measured by enzyme‐linked immunosorbent assay (ELISA) using sets of cytokine‐specific capture and detection mAb purchased from PharMingen (San Diego, CA, USA) as described [21]. For quantification of cytokine levels, standards for IL‐2, IL‐4, IL‐10 or IFN‐ γ (all purchased from Genzyme) were included in all ELISA determinations.…”

Section: Cytokine Determinationmentioning

confidence: 99%

The activity of inducible nitric oxide synthase in rejected skin xenografts is selectively inhibited by a factor produced by grafted cells

Holáň

Pindjáková

Zajı́cová

et al. 2005

Xenotransplantation

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Production of High Levels of Th1 and Th2 Cytokines in Mice with Acquired Transplantation Tolerance

Cited by 15 publications

References 0 publications

Corneal rat-to-mouse xenotransplantation and the effects of anti-CD4 or anti-CD8 treatment on cytokine and nitric oxide production

Corneal rat-to-mouse xenotransplantation and the effects of anti-CD4 or anti-CD8 treatment on cytokine and nitric oxide production

Induction of specific transplantation immunity by oral immunization with allogeneic cells

The activity of inducible nitric oxide synthase in rejected skin xenografts is selectively inhibited by a factor produced by grafted cells

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