Corneal rat-to-mouse xenotransplantation and the effects of anti-CD4 or anti-CD8 treatment on cytokine and nitric oxide production

Pindjáková, Jana; Vítová, Andrea; Krulová, Magdaléna; Zajı́cová, Alena; Filipec, Martin; Holáň, Vladimı́r

doi:10.1111/j.1432-2277.2005.00112.x

Cited by 17 publications

(22 citation statements)

References 37 publications

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“…Corneal xenotransplantation is not successful in animal models [17], and our data show that xenotransplantation of human corneal fibroblasts elicits a T‐cell‐mediated adaptive immune response together with development of corneal haze (Figs. 3–5).…”

Section: Discussionmentioning

confidence: 99%

Stem Cell Therapy Restores Transparency to Defective Murine Corneas

et al. 2009

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Corneal scarring from trauma and inflammation disrupts vision for millions worldwide, but corneal transplantation, the primary therapy for corneal blindness, is unavailable to many affected individuals. In this study, stem cells isolated from adult human corneal stroma were examined for the ability to correct stromal opacity in a murine model by direct injection of cells into the corneal stroma. In wild-type mice, injected human stem cells remained viable for months without fusing with host cells or eliciting an immune T-cell response. Human corneal-specific extracellular matrix, including the proteoglycans lumican and keratocan, accumulated in the treated corneas. Lumican-null mice have corneal opacity similar to that of scar tissue as a result of disruption of stromal collagen organization. After injection with human stromal stem cells, stromal thickness and collagen fibril defects in these mice were restored to that of normal mice. Corneal transparency in the treated mice was indistinguishable from that of wild-type mice. These results support the immune privilege of adult stem cells and the ability of stem cell therapy to regenerate tissue in a manner analogous to organogenesis and clearly different from that of normal wound healing. The results suggest that cell-based therapy can be an effective approach to treatment of human corneal blindness.

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Section: Discussionmentioning

confidence: 99%

Stem Cell Therapy Restores Transparency to Defective Murine Corneas

et al. 2009

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“…In rat-to-mouse corneal xenotransplantation, rejection was directly correlated to iNOS expression in the grafted button (22). These findings suggest that NO produced by iNOS in the corneal button may have potential endothelium toxicity mediated through nitrosative stress.…”

Section: Discussionmentioning

confidence: 89%

Nitrosative Stress and Corneal Transplant Endothelial Cell Death During Acute Graft Rejection

Bourges¹,

Torriglia²,

Valamanesh³

et al. 2007

Transplantation

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“…This observation underlines the role of the Th1 response in corneal allograft rejection. However, abundant Th2 cytokine genes (IL-4 and IL-10) are expressed, in addition to expression of Th1 cytokine genes 69. The rejection of xenogeneic corneal grafts appears to be mediated by proinflammatory cytokines, especially IFN-γ, that are released by CD4 + and/or CD8 + T cells at the graft site 33,69.…”

Section: The Immune Response To Corneal Xenograftsmentioning

confidence: 99%

Xenotransplantation-The Future of Corneal Transplantation?

Hara

Cooper

2011

Cornea

117

104

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Although corneal transplantation is readily available in the USA and certain other regions of the developed world, the need for human donor corneas worldwide far exceeds supply. There is currently renewed interest in the possibility of using corneas from other species, especially pigs, for transplantation into humans (xenotransplantation). The biomechanical properties of human and pig corneas are similar. Studies in animal models of corneal xenotransplantation have documented both humoral and cellular immune responses that play roles in xenograft rejection. The results obtained from the Tx of corneas from wild-type (i.e., genetically-unmodified) pigs into nonhuman primates have been surprisingly good and encouraging. Recent progress in the genetic manipulation of pigs has led to the prospect that the remaining immunological barriers will be overcome. There is every reason for optimism that corneal xenoTx will become a clinical reality within the next few years.

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Corneal rat-to-mouse xenotransplantation and the effects of anti-CD4 or anti-CD8 treatment on cytokine and nitric oxide production

Cited by 17 publications

References 37 publications

Stem Cell Therapy Restores Transparency to Defective Murine Corneas

Stem Cell Therapy Restores Transparency to Defective Murine Corneas

Nitrosative Stress and Corneal Transplant Endothelial Cell Death During Acute Graft Rejection

Xenotransplantation-The Future of Corneal Transplantation?

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