Production of ethanol from alkali-pretreated sugarcane bagasse under the influence of different process parameters

Abstract

The present study was designed to produce ethanol from sugarcane bagasse treated with 2.5% NaOH through a simultaneous saccharification and fermentation process using Saccharomyces cerevisiae G1. Various process parameters such as incubation temperature, incubation period, initial pH and nitrogen sources were studied to achieve the maximum yield of ethanol. The results showed that the optimum ethanol yield (5.90%) was achieved at an incubation temperature of 30°C, initial pH 5.5, inoculum size 3% (v/v), after an incubation period of 96 h. Among various nitrogen sources, ammonium sulphate was found to be the most suitable source for ethanol production. These findings indicate that the optimization of process parameters is necessary to make the fermentation process economical, and the medium designed in the present study could be exploited on a commercial scale after suitable processing.

Delignification of Lignocellulosic Biomass Sugarcane Bagasse by Using Ozone as Initial Step to Produce Bioethanol

“…Abo-state produced ethanol using SHF with enzymatic hydrolysis Trichoderma viride and candida tropicalis resulting to 226 kg (convert to %) ethanol per ton bagasse, while Aspergillus terreus for hydrolysis and S.cerevisiae for fermentation produced 185 kg per ton bagasse (convert to %) [31]. By using simultaneous saccharification and fermentation (SSF), it produced better results, the fermentation performed at 30ºC for 96 hour in the presence of ammonium nitrate generated 5.90% yield [32]. However, saccharification step is conducted by commercial cellulose enzyme known to be expensive.…”

Pretreatment of maize straw with Pleurotus ostreatus and Lentinus squarrosulus for bioethanol production using Saccharomyces cerevisiae

“…water until the pH of the filtrate was about 7. The residue was dried at 105 °C until a constant weight was obtained (Irfan et al, 2011;Nadeem et al, 2015). The alkali-pretreated sample was then degraded with a mushroom (Pleurotus ostreatus), which already had a recorded better degradability in terms of the released reducing sugars for 21 d; as described before in the step of pretreatment of MS with the white rot fungi.…”

“…The alkali-pretreated sample was then degraded with a mushroom (Pleurotus ostreatus), which already had a recorded better degradability in terms of the released reducing sugars for 21 d; as described before in the step of pretreatment of MS with the white rot fungi. After the degradation, the samples were dried at 105 °C until a Novel Research in Microbiology Journal, 2021 constant weight was obtained, and then kept in an airtight nylon until further use (Adenipekun and Fasidi, 2005;Nadeem et al, 2015). After drying, the reducing sugar contents and sugar profiles of the degraded samples were determined, and then these samples were used for bioethanol production.…”

“…; allowed to cool, and then inoculated individually with 2 % inoculum of 1.0 MacFarland standard of S. cerevisiae SA01 and S. cerevisiae SA02. All treatments were incubated at 30 °C for 5 d (Nadeem et al, 2015). An aliquot of 100 ml of the fermenting samples was withdrawn every 24 h for estimation of the reducing sugars and the bioethanol contents.…”

Hydrodynamic cavitation as a new approach for sugarcane bagasse pretreatment aiming to second generation ethanol production

“…The efficiency of fermentation in that study was lower than obtained in our study (94%), which could be associated to optimal temperature used for fermentation process. In our work, temperature was maintained at 30 °C in the fermentation column during the whole process and this temperature has to do with the ethanol yield by Saccharomyces cerevisiae G1 as reported by Nadeem et al (2015). It was reported that the maximal yield (0.14 g/g) was achieved at 30 °C, it further reduced in 2% and 32.6% with an increase to 35°C and 40°C, respectively.…”