Delignification of Lignocellulosic Biomass Sugarcane Bagasse by Using Ozone as Initial Step to Produce Bioethanol

Abstract

The choice of pretreatment is a very important in bioethanol production from lignocellulosic biomass. This helps to eliminate lignin partition between cellulose and hemicellulose. However, various methods generate diverse effects on the material structure and composition. The purpose of this study, therefore, was to delignify sugarcane bagasse by ozonolysis, followed by hydrolysis and fermentation. Also, the morphology of the samples was analyzed using SEM, while hemicellulose, cellulose, and lignin content were characterized by the Chesson process. The sample was hydrolyzed using 1% (v/v) sulfuric acid and the bioethanol fermented with Saccharomyces cerevisiae was detected by gas chromatography. Furthermore, ozone was applied for 90 minutes at pH 3.0 in the delignification process. This produces cellulose, hemicellulose, and lignin estimated at 59%, 22%, and 6%, respectively. However, ozonolysis was employed to reduce lignin for up to 217%. Meanwhile, the hydrolysed samples were known to rapidly decrease the reducing sugar from 19.342 to 2.86 mg/L after heating at 100ºC. Subsequently, the fermentation stage recorded the highest ethanol production, estimated at 0.79% (v/v). The result showed lignin removal was conducted in an eco-friendly and efficient condition. Therefore, the need for further study is possible in order to optimize certain parameters for maximum bioethanol production.

Comparative Analysis of Acidic and Alkaline Pretreatment Techniques for Bioethanol Production from Perennial Grasses

“…Accurate compositional analysis of LCB is vital for biofuel production, impacting parameters such as ethanol yield, recovery rates, and techno-economic assessments [48,49]. Reliable analytical techniques, such as those provided by the National Renewable Energy Laboratory (NREL) and the Chesson standard method, are employed for quantifying carbohydrates, lignin, and ash in biomass [49][50][51][52][53][54]. Table 2 summarizes the composition of commonly employed grasses in the biofuel industry, as documented in the literature.…”

The Effects of Ozonolysis on Oil Palm Fruit Mesocarp Delignification

“…OPFM powder was weighed for 5 g each and wetted with 10% H 2 O by weight of the material pH of 3 (Hermansyah et al, 2021). The wetted sample was put into the ozone reactor and the flow rate was adjusted with variations of 1 Lmin -1 , 2 Lmin -1 , and 3 Lmin -1 .…”

“…In this study, the optimum reaction time for ozonolysis delignification was 15 min with 42.03% of lignin removal. The reaction time influences the extent of lignin degradation achieved during the treatment process, with longer reaction times generally leading to more extensive lignin removal (Baig, 2022;Hermansyah et al, 2021). However, this increased the expense of delignification.…”

Synthesis Lignin-Mg(OH)₂ from Lignin Bagasse as Green Adsorbent of Heavy Metal Pb (II)

“…The amount of waste generated as solid, liquid, or gas will be proportional to sugarcane production activities. Bagasse is the most solid waste from sugarcane production [7,8].…”