Pretreatment of maize straw with Pleurotus ostreatus and Lentinus squarrosulus for bioethanol production using Saccharomyces cerevisiae

Fasiku, Samuel Adedayo; Wakil, Sherifah Monilola

doi:10.21608/nrmj.2021.209731

Cited by 3 publications

(3 citation statements)

References 13 publications

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“…Lignocellulosic materials are abundant in nature and are principally made up of cellulose, hemicellulose and lignin [1, 2]. Hemicellulose is the second most plentiful biological polymer in the world after cellulose and the main component of hemicellulose is xylan, which is broken down to xylose by the enzyme called xylanase [3].…”

Section: Introductionmentioning

confidence: 99%

Production of xylanase by Aspergillus niger GIO and Bacillus megaterium through solid-state fermentation

Fasiku

Bello

Odeniyi

2023

Access Microbiology

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Xylanase breaks xylan down to xylose, which is used in industries such as pulp and paper, food and feed, among others. The utilization of wastes for xylanase production is economical, hence this work aimed at producing xylanase through solid-state fermentation and characterizing the enzyme. Xylanase-producing strains of Bacillus megaterium and Aspergillus niger GIO were inoculated separately in a 5 and 10 day solid fermentation study on maize straw, rice straw, sawdust, corn cob, sugarcane bagasse, conifer litters, alkaline-pretreated maize straw (APM) and combined alkaline and biological-pretreated maize straw, respectively. The best substrate was selected for xylanase production. The crude enzyme was extracted from the fermentation medium and xylanase activity was characterized using parameters such as temperature, cations, pH and surfactants. Among different substrates, the highest xylanase activity of 3.18 U ml−1 was recorded when A. niger GIO was grown on APM. The xylanase produced by A. niger GIO and B. megaterium had the highest activities (3.67 U ml−1 and 3.36 U ml−1) at 40 °C after 30 and 45 min of incubation, respectively. Optimal xylanase activities (4.58 and 3.58 U ml−1) of A. niger GIO and B. megaterium , respectively, were observed at pH 5.0 and 6.2. All cations used enhanced xylanase activities except magnesium ion. Sodium dodecyl sulfate supported the highest xylanase activity of 6.13 and 6.90 U ml−1 for A. niger GIO and B. megaterium , respectively. High yields of xylanase were obtained from A. niger GIO and B. megaterium cultivated on APM. The xylanase activities were affected by pH, temperature, surfactants and cations.

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Section: Introductionmentioning

confidence: 99%

Production of xylanase by Aspergillus niger GIO and Bacillus megaterium through solid-state fermentation

Fasiku

Bello

Odeniyi

2023

Access Microbiology

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“…Studies on the role of spent mushroom substrates after this cultivation produces many important materials, such as enzymes [12,19,126,127], biomass [118,128,129], bioethanol [19,127,130], feed ingredients, and functional foods [61,131,132]. Spent mushroom substrates can be recycled as a substrate for the "new cultivation cycle" of mushrooms, a feedstock for producing the second generation of biofuels, a bio-control agent, a biofertilizer, and for soil amendment [133][134][135]; 2.…”

Section: Mediamentioning

confidence: 99%

Green Biotechnology of Oyster Mushroom (Pleurotus ostreatus L.): A Sustainable Strategy for Myco-Remediation and Bio-Fermentation

et al. 2022

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The field of biotechnology presents us with a great chance to use many organisms, such as mushrooms, to find suitable solutions for issues that include the accumulation of agro-wastes in the environment. The green biotechnology of mushrooms (Pleurotus ostreatus L.) includes the myco-remediation of polluted soil and water as well as bio-fermentation. The circular economy approach could be effectively achieved by using oyster mushrooms (Pleurotus ostreatus L.), of which the substrate of their cultivation is considered as a vital source for producing biofertilizers, animal feeds, bioenergy, and bio-remediators. Spent mushroom substrate is also considered a crucial source for many applications, including the production of enzymes (e.g., manganese peroxidase, laccase, and lignin peroxidase) and bioethanol. The sustainable management of agro-industrial wastes (e.g., plant-based foods, animal-based foods, and non-food industries) could reduce, reuse and recycle using oyster mushrooms. This review aims to focus on the biotechnological applications of the oyster mushroom (P. ostreatus L.) concerning the field of the myco-remediation of pollutants and the bio-fermentation of agro-industrial wastes as a sustainable approach to environmental protection. This study can open new windows onto the green synthesis of metal-nanoparticles, such as nano-silver, nano-TiO2 and nano-ZnO. More investigations are needed concerning the new biotechnological approaches.

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“…Second-generation biofuels are produced from non-edible agricultural wastes however, reducing sugars needed for the generation of biofuel in these wastes are not readily released from them [1][2][3]. Therefore, it is necessary to pretreat non-edible agricultural wastes to obtain simple sugars needed for the production of biofuel.…”

Section: Introductionmentioning

confidence: 99%

Degradation of lignocellulosic substrates by Pleurotus ostreatus and Lentinus squarrosulus

Fasiku

Wakil

Alao³

2023

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Lignocellulosic substrates are wastes in the environment whose reducing sugars are not readily available for use. Biological pretreatment is the use of microorganisms and/or their metabolites to break down substrates to obtain simple sugars which is also cheap compared with other pretreatment techniques. This work is aimed at degrading lignocellulosic substrates with higher mushrooms to obtain simple sugars that could be used as raw materials for other industrial processes. The two mushrooms [Pleurotus ostreatus (PO) and Lentinus squarrosulus (LS)] with the ability to produce cellulase, xylanase, and lignase were used for degradation of lignocellulosic substrates [groundnut shell (GS), maize cob (MC), maize straw (MS), rice straw (RS) and sugarcane bagasse (SB)]. The residual extractives, cellulose, hemicellulose, lignin, and reducing sugar contents were determined every 7 days. Least extractives (1.12 %), hemicellulose (15.09 %), lignin (17.60 %), and cellulose (5.60 %) were recorded in PO-degraded MS, POLS-degraded GS, LS-degraded GS, and PO-degraded MS at 28, 35, 49 and 42 days of degradation, respectively. The highest reducing sugar contents (mg/g) obtained in GS (11.83), MS (27.03), SB (28.70), and RS (37.96) were recorded when degraded by PO for 49, 14, 7, and 49 days, respectively while that of MC (13.32) was recorded when degraded by LS for 42 days. Reducing sugar obtained was higher from sole degradation with PO compared with LS and POLS. Degraded MS, RS, and SB had better yield of reducing sugar than GS and MC. The amount of reducing sugar released varied with substrates, organisms, and degradation time.

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Pretreatment of maize straw with Pleurotus ostreatus and Lentinus squarrosulus for bioethanol production using Saccharomyces cerevisiae

Cited by 3 publications

References 13 publications

Production of xylanase by Aspergillus niger GIO and Bacillus megaterium through solid-state fermentation

Production of xylanase by Aspergillus niger GIO and Bacillus megaterium through solid-state fermentation

Green Biotechnology of Oyster Mushroom (Pleurotus ostreatus L.): A Sustainable Strategy for Myco-Remediation and Bio-Fermentation

Degradation of lignocellulosic substrates by Pleurotus ostreatus and Lentinus squarrosulus

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