Production of ethanol by Enterobacter sp. EtK3 during fruit waste biotransformation

Sarkar, Debapriya; Prajapati, Sushant; Poddar, Kasturi; Sarkar, Angana

doi:10.1016/j.ibiod.2019.104795

Cited by 31 publications

(14 citation statements)

References 37 publications

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“…These statistics signified the good fit of the model. The ANOVA analysis ( Table 4 , Table 5 ) also suggested the model is signified with considerable F and p values and lack of fit was also found non-significant [ 43 ]. Biomass = 0.0951−0.0006A + 0.0056B + 0.014C - 0.0177AB - 0.0055AC + 0.0013BC + 0.0162A 2 + 0.0043B 2 - 0.0052C 2 - 0.005ABC + 0.0396A 2 B - 0.0252A 2 C - 0.0232AB 2 where A, B, and C are temperature, pH, and inoculum percentage, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The pigment-producing colonies of BPW19 were isolated and pure colonies of the strain was obtained by subsequent rounds of quadrant streaking method on sterile agar plates with 1.5 % (w/v) of agar. The strain was further identified by analyzing its 16S rRNA gene [ 43 ]. The genomic DNA of the strain was isolated from the cell culture pellet using a standard enzymatic protocol with some modifications along with a Gram-positive control, Bacillus subtilis, and a Gram-negative control, Escherichia coli .…”

Section: Methodsmentioning

confidence: 99%

“…To optimize the biomass production and cellular growth of the isolated strain BPW19, a two-level, three factorial central composite design (CCD) was obtained using Design-Expert software (Trial version). For the biomass production, the three main contributing factors were taken as the incubation temperature, pH and inoculum percentage which are represented as coded values of A, B, and C, respectively [ 43 , 53 ]. The levels for A, B, and C were considered as 25 °C and 37 °C, 6 and 8, 1.5, and 3.5, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Production, purification, and process optimization of intracellular pigment from novel psychrotolerant Paenibacillus sp. BPW19

Padhan

Poddar

Sarkar

et al. 2021

Biotechnology Reports

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Production, purification, and process optimization of intracellular pigment from novel psychrotolerant Paenibacillus sp. BPW19

Padhan

Poddar

Sarkar

et al. 2021

Biotechnology Reports

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“…The bacteria were characterized by Gram staining using Gram Staining Kit (K001) from Himedia, India [21]. The spore-forming nature of the isolates was identified by the standard procedure using malachite green for 10 min on the pre-heat-fixed smear that stains up the endospores produced by the bacteria on exposure to stress [22].…”

Section: Biochemical Characterizationmentioning

confidence: 99%

Purification and optimization of pink pigment produced by newly isolated bacterial strain Enterobacter sp. PWN1

et al. 2021

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Pigment-producing bacteria were isolated from kitchen wastewaters of the National Institute of Technology, Rourkela. A pink non-virulent bacterial strain PWN1 was selected based on the India Ink Broth and Coomassie Brilliant Blue (R-250) dye assay. According to morphological and biochemical characterization, the strain PWN1was a Gram-negative, rod-shaped, motile, non-coliform bacterium and could utilize only glucose and adonitol as sole carbon source. The pigment was found to be a growth-associated product, and the pigment production was accelerated after 40 h of bacterial culture. Further, 16S rRNA gene-based molecular identification showed its similarity with Enterobacter sp. The pigments were extracted by the solvent extraction method using chloroform and ethanol (3:1). The extracted pigments were then purified through thin-layer chromatography and column chromatography. To maximize pigment production, the culture condition was optimized for maximum biomass production using statistical software Design Expert v13. A quadratic model was structured describing the process efficiently and it suggested a moderate temperature, pH, and a high inoculum concentration which generated biomass of 3.81 ± 0.02 g/L. At optimized condition, 1 L of cell culture produced 3.77 g of biomass which produced a crude pigment of 0.234 g after solvent extraction and 0.131 g after column chromatography, implying a yield of 6.2% for crude pigment and 3.47% for purified pigment from biomass. The yield of the obtained pigment was high enough to draw interest for industrial production, although the application of the pigment is considerable for further study.

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“…SARKAR et al, 2019)three-factor optimization study using Response Surface Methodology (RSM. Especialmente, o bagaço de mandioca é um resíduo obtido do processo de extração de amido da mandioca.…”

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Resina Benzoxazina: Obtenção E Potencial De Aplicação No Setor Aeroespacial

Bandeira¹,

Trofino²,

Montoro³

et al. 2020

Avanços Das Pesquisas E Inovações Na Engenharia Química

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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Production of ethanol by Enterobacter sp. EtK3 during fruit waste biotransformation

Cited by 31 publications

References 37 publications

Production, purification, and process optimization of intracellular pigment from novel psychrotolerant Paenibacillus sp. BPW19

Production, purification, and process optimization of intracellular pigment from novel psychrotolerant Paenibacillus sp. BPW19

Purification and optimization of pink pigment produced by newly isolated bacterial strain Enterobacter sp. PWN1

Resina Benzoxazina: Obtenção E Potencial De Aplicação No Setor Aeroespacial

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