Production, purification, and process optimization of intracellular pigment from novel psychrotolerant Paenibacillus sp. BPW19

Heritage and Sustainable Development

et al. 2022

The study was aimed to assess culturable bacterial endophytes from the medicinal plant Aloe vera, their antimicrobial spectra against pathogens, and the potential of bacterial endophytes in textile and paper dyeing. Culturable seventeen bacterial endophytes were isolated from the Aloe vera plant out of which 16 showed varied antimicrobial activity against both human pathogens i.e., bacteria & fungi E. coli, S. pyogenes, acne bacterial isolate (ABI), A. niger, and F. oxysporum. Simultaneously, the bacterial endophyte ENDB3 is producing extracellular green-brown color pigment under submerged (SmF) condition and the extracted pigment has shown promising results in textile and paper dyeing at lab scale without using mordant. All the bacterial endophytes showed resistance against standard antibiotics (penicillin G P(10 units), Oxacillin (1 mcg), Cephalathin (30 mcg), Clindamycin (2 mcg), Erythromycin (15 mcg), and Amoxyclav (30 mcg)) at the specific concentration used. Concludingly, bacterial endophyte ENDB3 is found capable to produce bioactive molecules with pharmaceutical and dyeing industries which may provide a new path in the pursuit of new biological sources of drug and natural dyeing candidates. Hence, we suggest further evaluation and characterization of their bioactive molecules for pharmacological and dyeing potential.

Section: Discussionmentioning

confidence: 99%

Bacterial endophytes of aloe vera and their potential applications

Chitranshi

Gupta

Heritage and Sustainable Development

et al. 2022

“…This edited sequence was then analyzed for local sequence alignment using BLAST (Basic Local Alignment Searching Tool) provided by the National Centre for Biotechnology Information (NCBI) to identify the related strains. MEGA X software was used for further multiple sequence alignment using the CLUSTALW tool and a phylogenetic tree was constructed using the neighbor‐joining method 21 . For the construction of the phylogenetic tree, Rhodobacter capsulatus JA232 AM90347.1 was considered to form an outgroup.…”

Section: Methodsmentioning

confidence: 99%

“…MEGA X software was used for further multiple sequence alignment using the CLUSTALW tool and a phylogenetic tree was constructed using the neighbor-joining method. 21 For the construction of the phylogenetic tree, Rhodobacter capsulatus JA232 AM90347.1 was considered to form an outgroup.…”

Section: Antibiotic Susceptibility Profilementioning

confidence: 99%

Synthesis and characterization of polyhydroxyalkanoates from soil bacterium Bacillus sp. PhNs9

Patil

J of Chemical Tech & Biotech

Poddar

et al. 2022

Self Cite

Background: Synthetic plastic pollution has increased tremendously over the past few decades. Supplanting synthetic plastics with a biodegradable alternative could save the biosphere from the drastic effects of plastic pollution. Polyhydroxyalkanoates (PHA) have been identified with the potential to become a feasible option.Results: A bacterial strain isolated from pavement soil was investigated for its PHA production ability. The isolated strain was found to be non-virulent, and susceptible to various antibiotics, especially cefalexin, tetracycline, chloramphenicol, norfloxacin and ofloxacin. The carbohydrate utilization profile revealed that isolated bacteria can utilize different carbohydrates with a variable rate of consumption. The 16S rRNA identification revealed that the strain was related to the Bacillus genus. The produced PHA was predicted to be polyhydroxybutyrate as suggested by differential scanning calorimetry and NMR, Fourier transform infrared and Raman spectroscopies. Moreover, the hydrophobic nature of the PHA produced was confirmed by contact angle measurement. The growth, production and substrate utilization kinetics revealed the specific growth rate of Bacillus sp. PhNs9 was 0.07 h −1 , specific biomass productivity was 0.06 g L −1 h −1 and specific PHA productivity was 0.05 g L −1 h −1 . The yield of PHA from biomass was found to be 0.67 g per gram of biomass.Conclusions: The yield of PHA from the isolated strain was found to be higher than that of most earlier reports. Hence the findings of this study could catalyze the progress to completely move away from the use of synthetic plastics and replace them with an economical and sustainable solution.

“…The isolated bacterial strain was biochemically characterized by its indole production activity (IP), methyl red test (MR), Voges–Proskauer test (VP), and citrate utilization test (CU) using the IMViC test kit (Himedia, India). The rigidity of preferred carbohydrate as a carbon source of bacterial strain was evaluated by growing the bacterial strain in different media adding a sole carbon source, such as adonitol, arabinose, glucose, lactose, mannitol, rhamnose, sorbitol, and sucrose (Padhan et al., 2021).…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of an antibacterial compound producing Stenotrophomonas strain from sewage water, production optimization, and its antibiotic potential evaluation

Environmental Quality Mgmt

Nanda

Poddar

et al. 2021

Self Cite

The increasing antibiotic resistance due to inappropriate administration of antibiotics is a huge concern for the scientific society, recurring the demand for novel antibiotics against antibiotic‐resistant bacteria. In this study, ABN18 bacterial strain was isolated from sewage wastewater with antibacterial substance production ability and was identified as Stenotrophomonas sp. by partial 16S rRNA sequencing. The isolated bacterial strain was Gram‐negative, long oval‐shaped, motile, nonendospore forming with an average length of 1.7025 μm determined by environmental scanning electron microscope (ESEM). Stenotrophomonas sp. ABN18 was found to have a flexible carbohydrate preference, only unable to grow in sorbitol. The strain showed moderate antibiotic resistance with significant sensitivity against antibiotics such as ceftazidime, doxycycline hydrochloride, and rifampicin. Antibacterial activity of ABN18 was found to be more active against Gram‐negative strains as compared with Gram‐positive bacterial strains. The well‐diffusion study confirmed the extracellular production of the antibacterial substance and its presence in the fermentation broth. Moreover, the temperature, pH, and incubation time were optimized at 33.17°C, 7.45, and 88.8 h, respectively, to maximize antibacterial substance production, and the highest zone of inhibition diameter of 22 mm against E. coli was achieved.