Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon

Abstract

Abstract. Septiani D, Suryadi H, Mun’im A, Mangunwardoyo W. 2019. Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon. Biodiversitas 20: 3539-3544. Cellulase is one of hydrolytic enzymes that breakdown cellulose into glucose. Cellulases are promising to be applied in natural products which may improve the yield of bioactive in plant extract through cellulose depolymerization. Cellulases from mixed culture of Aspergillus niger and Trichoderma reesei can produce a high cellulase activity because of the synergism activity among endoglucanase, exoglucanase, and also β-glucoside. Cellulase production and partial purification of monoculture and mixed culture (1:1) of these fungi on carboxymethylcellulose media were investigated in this study. Total cellulase activity was measured by filter paper assay followed by protein estimation with Bradford method. The crude extract of Aspergillus niger monoculture has the highest cellulase activity (0.131 U/mL, P<005) followed by mixed culture (0.109 U/mL) and Trichoderma reesei (0.106 U/mL). The cellulase activity of partially purified cellulase from mixed culture significantly increased (0.335, 0.348, 0.374 U/mL, P<0.05) compared to crude extract along with stepwise addition of ammonium sulfate. Cellulase activity of mixed culture at 80% ammonium sulfate increase up to 2.238-fold and showed highest value (P<0.05) compared to monocultures. In conclusion, combination of Aspergillus niger and Trichoderma reesei fungi in carboxymethyl cellulose media followed by 80% ammonium sulfate precipitation can be a promising cellulase production with high cellulase activity.

Microbial Secondary Metabolites via Fermentation Approaches for Dietary Supplementation Formulations

“…Continuous advancements in biotechnology and synthetic biology are continually expanding the possibilities for microbiological production of carotenoids, offering sustainable and efficient alternatives to traditional methods [138]. The followings steps are involved in the carotenoid's extraction process from microorganisms [138][139][140][141][142][143][144][145][146]:…”

Isolation and Evaluation of Endophytic Fungi from Paddy (Oryzasativa) for Antifungal Activity Against Fusarium sp.

“…In addition, Trichoderma sp produced cellulose enzymes that can damage the cell walls of pathogenic fungi. In this case, Septiani et al [22] stated that Trichoderma sp can produce cellulase to break down cellulose into glucose. Cellulose is the main component of the cell wall of the pathogenic fungus.…”

Improving Enzyme-Assisted Extraction of Brazilin from Sappanwood (Caesalpinia Sappan L.) Extract by Fungal Cellulase

“…17 Cellulase from Aspergillus niger, Trichoderma reesei, and its mix culture have been produced by submerged-fermentation method through previous research. 13 That obtained the highest cellulase activity was from A.niger cellulase (0.467 U/mL). From that result, in this study, cellulase from A.niger is used to extract sappanwood by enzyme-assisted extraction (EAE).…”

“…Pharmacognosy Journal, Vol 14, Issue 1, Jan-Feb, 2022 by Badan Penelitian Tanaman Rempah dan Obat (BALITTRO), Bogor, West Java. Dried cellulase enzyme from Aspergillus niger UICC 371 from carboxymethyl cellulose submerged fermentation's media (Septiani et al, 2019). All the chemicals were analytical grade, aqua pro injection (IKA Pharmaceutical, Jerman), methanol HPLC grade (Merck, Jerman), acetonitrile HPLC grade (Merck, Jerman), acetic acid glacial (Merck, Jerman), brazilin standard (Sigma Aldrich, USA), 95% ethanol (Brataco, Indonesia), oven (WTB Binder, Germany), analytical balance (Acculab, USA), hotplate stirrer (Corning, USA), pH meter (Hanna, USA), air shaking incubator (Heidolph, Germany), laminar airflow (Kubota, Japan), ultrasonic bath (Krisbow), digital scales (Vibra HT), hotplate stirrer (IKA C-MAG), high performance liquid chromatography (Shimadzu LC-20AT, Japan), column C 18 4.6 mm×150 mm (Inertsil), rotary vacuum evaporator (Buchi), centrifuge (Hettich Zentrifugen), micropore 0,45 µm (Whatman), and syringe filter (Agilent).…”