Production of biologically active hydroxytyrosol rich extract <i>via</i> catalytic conversion of tyrosol

Neji, Soumaya Bouguerra; Bouaziz, Mohamed

doi:10.1039/d1ra08875k

Cited by 7 publications

(8 citation statements)

References 37 publications

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“…In this research, the contents of 17 secondary metabolites in fruiting bodies were highest, such as phenylethylamine, midodrine, syringic acid, nandrolone, hydroxytyrosol, oxazepam, anileridine, agmatine, and carbidopa. These metabolites present various pharmacological properties such as antioxidant, antimicrobial, anti‐inflammatory, protective agents for the nervous system, and enforcing immunity system 48,49 . Fruiting bodies were rich in active secondary metabolites, which may have various pharmacological activities.…”

Section: Discussionmentioning

confidence: 99%

“…These metabolites present various pharmacological properties such as antioxidant, antimicrobial, antiinflammatory, protective agents for the nervous system, and enforcing immunity system. 48,49 Fruiting bodies were rich in active secondary metabolites, which may have various pharmacological activities. There were more stachydrine, alprenolol and phenibut in mycelia than those in the other three cultures.…”

Section: Saccharides Hold a Large Proportion Inmentioning

confidence: 99%

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Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Wang

Yang

Tong

et al. 2023

Phytochemical Analysis

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IntroductionOphiocordyceps gracilis, a type of edible and medicinal fungus, exhibits multiple health‐promoting effects. Due to the scarcity of natural O. gracilis, artificial cultures have been developed as its substitutes. However, lacking comprehension of the metabolite composition of cultures limits its utilisation.ObjectiveThis research aimed to evaluate the nutritional and medicinal value of four cultures of O. gracilis by analysing their metabolite composition. In addition, metabolic pathways in mycelia and fruiting bodies were analysed to explore fruiting body formation mechanism at metabolic level.MethodThe mycelia, fermentation broth, spore powder and fruiting bodies of O. gracilis were cultivated in this study. Their metabolite composition was compared using an untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry (LC–MS/MS).ResultsPrincipal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS‐DA) showed that the four cultures have noticeable differences in metabolite composition. A total of 612 metabolites were identified, among which 159 metabolites showed significant differences, and these differential metabolites were classified into 13 categories. The metabolites in the fruiting bodies were the most abundant compared with other cultures. However, each culture had its own advantages and significantly accumulates some active metabolites respectively. Pearson's correlation analysed the mutual relationship among metabolites. In addition, seven metabolic pathways were closely related to fruiting body formation, such as “Biosynthesis of plant secondary metabolites”, “amino acids metabolism”, “tricarboxylic acid (TCA) cycle”.ConclusionThis study offered a reference to mycelia, fermentation broth, spore powder and fruiting bodies of O. gracilis as health‐promoting functional foods and medicine.

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Section: Discussionmentioning

confidence: 99%

Section: Saccharides Hold a Large Proportion Inmentioning

confidence: 99%

Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Wang

Yang

Tong

et al. 2023

Phytochemical Analysis

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“…The differences in the antioxidant activity observed between the modified oils prepared in this work were ascribed to the chemical structure of the three phenolic alcohols. HTYR has two aromatic hydroxyl groups (catecholic structure) in contrast to the one of HVA and VA, which subserve its ability to act as a stronger antioxidant [32,33]. Previous research has also demonstrated that the enzymatic process of lipophilization enhanced the antioxidant activity of the raw oils and acylating agents that were conjugated with ethyl ferulate [19].…”

Section: Antioxidant Evaluation Of the Control And Modified Oilsmentioning

confidence: 99%

“…(catecholic structure) in contrast to the one of HVA and VA, which subserve its ability to act as a stronger antioxidant [32,33]. Previous research has also demonstrated that the enzymatic process of lipophilization enhanced the antioxidant activity of the raw oils and acylating agents that were conjugated with ethyl ferulate [19].…”

Section: Antioxidant Evaluation Of the Control And Modified Oilsmentioning

confidence: 99%

Enzymatic Modification of Pomace Olive Oil with Natural Antioxidants: Effect on Oxidative Stability

et al. 2023

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Enzymatic lipophilization has been proposed as a cost-effective strategy to produce new liposoluble antioxidant compounds. In this study, modified oils rich in structured phenolipids were prepared via one-pot enzymatic acylation of hydroxytyrosol (HTYR), vanillyl alcohol (VA) and homovanillyl alcohol (HVA) with pomace olive oil (POO) in solvent-free conditions using immobilized lipase on biogenic nanoparticles. The effect of temperature (30–70 °C) and enzyme concentration (0.1–1%, w/w) on the efficiency of the bioprocess as well as the reusability of the nanobiocatalyst were thoroughly investigated. The modified oils exhibited increased antioxidant activity compared to the control oil according to DPPH and CUPRAC assays (p < 0.05). The oxidative stability of pomace olive oil was also significantly enhanced after modification, as depicted by the K232 values and TBARS contents under accelerated oxidation at 60 °C (p < 0.05). Moreover, a fortified mayonnaise containing modified oil with HTYR was prepared that was noticeably stable compared to the control mayonnaise at 28 °C for 5 months (p < 0.05). Enzymatically modified oils have great potential for application in the nutraceutical and food industry, encouraging the exploitation of immobilized lipases as effective and green catalytic tools.

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“…Following purification, a minimum of 3 kg of HT with a purity of 90-95% was obtained. Neji et al [4] catalyzed the generation of HT extracts from tyrosol through a wet hydrogen peroxide-catalyzed oxidation reaction using montmorillonite Key Success Factors (KSF) as a solid acid catalyst at room temperature. Papageorgiou et al [5] employed a solid-liquid extraction method to obtain HT from oleuropein, utilizing a water-ethanol mixture, hydrochloric acid hydrolysis, and optimized ethyl acetate extraction.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis and Biotechnological Synthesis of Hydroxytyrosol

Tang,

Wang,

Gong

et al. 2023

Preprint

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Background： Hydroxytyrosol (HT), a phenolic compound derived from plants, exhibits robust antioxidant activity and possesses pharmacological properties, including anti-inflammatory, anti-cancer, anti-bacterial, and anti-viral effects. As a result, HT has gained significant attention as an excellent nutraceutical and food additive. Purpose and scope： Currently HT is mainly produced by plant extraction and chemical synthesis. With the gradual development of biosynthesis technology and the increasing concern for food health, biosynthesis of HT has gained more and more attention from researchers. Summary and conclusion: Biotechnological synthesis of HT offers distinct advantages over plant extraction and chemical synthesis, such as being environmentally friendly, safe, and cost-effective. This comprehensive review focuses specifically on the biosynthetic pathway of HT and recent advancements in biotechnological synthesis of HT. The review also discusses the potential applications of HT as a nutraceutical. Through a comprehensive analysis of the biosynthesis and biotechnological synthesis of HT, this review contributes to the growing body of knowledge in this field and paves the way for sustainable and scalable production of this versatile compound.

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Production of biologically active hydroxytyrosol rich extract via catalytic conversion of tyrosol

Abstract: An effective and economical process was established to produce hydroxytyrosol, a natural antioxidant molecule.

Cited by 7 publications

References 37 publications

Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Enzymatic Modification of Pomace Olive Oil with Natural Antioxidants: Effect on Oxidative Stability

Biosynthesis and Biotechnological Synthesis of Hydroxytyrosol

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