Specific chemical
reactions only happen in the tumor region and produce abundant special
chemicals to in situ trigger a train of biological and pathological
effects that may enable tumor-specific curative effects to treat cancer
without causing serious side effects on normal cells or organs. Chemodynamic
therapy (CDT) is a rising tactic for cancer therapy, which induces
cancer cell death via a localized Fenton reaction. However, the tumor
therapeutic effect is limited by the efficiency of the chemical reaction
and relies heavily on the catalyst. Here, we constructed hollow porous
carbon coated FeS2 (HPFeS2@C)-based nanocatalysts
for triple-enhanced CDT. Tannic acid was encapsulated in HPFeS2@C for reducing Fe3+ to Fe2+, which
had a better catalytic activity to accelerate the Fenton reaction.
Afterward, glucose oxidase (GOx) in nanocatalysts could consume glucose
in the tumor microenvironment and in situ synchronously produce H2O2, which could improve Fenton reaction efficiency.
Meanwhile, the consumption of glucose could lead to the starvation
effect for cancer starvation therapy. The photothermal effects of
HPFeS2@C could generate heat, which further sped up the
Fenton process and implemented synergetic photothermal therapy/starvation
therapy/CDT. The biodistribution of nanoparticles was investigated
by multimodal magnetic resonance, ultrasound, and photoacoustic imaging.
These nanocatalysts could trigger the catalytic Fenton reaction at
a high degree, which might provide a good paradigm for nanocatalytic
tumor therapy.
By incorporation of a specific NO-binding group, 2-amino-3'-dimethylaminobiphenyl, into a Bodipy dye, fluorescent probe was constructed, which exhibited high selectivity for NO over other ROS/RNS as well as DHA, AA and MGO.
Enterocytozoon hepatopenaei (EHP) is one of the most serious pathogens in shrimp farming. This study combines recombinase polymerase amplification (RPA) with the Argonaute from Pyrococcus furiosus (PfAgo) and establishes a sensitive and reliable method for on-site detection of EHP. With careful screening of gDNA and optimization of the reaction, the method shows a good specificity and reaches a sensitivity of single copy per reaction, which is higher than the sensitivity of the currently available molecular assays. The whole procedure can be finished within 1.5 h including the sample processing time and only requires minimum laboratory support, which is user-friendly for on-site environments. This is the first application of PfAgo for the diagnosis of infectious diseases in seafood supply chains. It provides a reliable method for on-site detection of EHP in shrimp farms and establishes a groundwork for multiplex detection of important pathogens in seafood farming using PfAgo.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.