Production of Anti-Amyloid β Antibodies in Mice Fed Rice Expressing Amyloid β

Nojima, Jun; Ishii-Katsuno, Rika; Futai, Eugene; Sasagawa, Noboru; Watanabe, Yoshihisa; Yoshida, Taiji; Ishiura, Shoichi

doi:10.1271/bbb.100861

Cited by 11 publications

(6 citation statements)

References 20 publications

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“…We reported previously that mice were able to produce antibodies against Aβ following oral immunization and the induction of immunotolerance to vehicle proteins. 11) Therefore, the side effects induced by oral adjuvant can be avoided for safer future vaccine therapies.…”

Section: Discussionmentioning

confidence: 99%

“…Oral vaccinations using plant-derived antigens have fewer side effects than those administered by injection. 10,11,22) In addition to the benefit of immunological safety, plant-derived vaccines are considered to be safer and cheaper than those produced from animal cells or microbes. A vaccine produced from animal cells or microbes requires refinement during the manufacturing process, because animal cells may contain viruses and prions that might infect humans.…”

Section: Discussionmentioning

confidence: 99%

“…When Aβ-containing plants (green pepper, 10) rice, 11,13) and potato 15) ) were administered to mice, their serum anti-Aβ antibody titers were elevated. In these experiments, CTB was added as an adjuvant.…”

Section: Discussionmentioning

confidence: 99%

“…Aβ has been expressed in edible plants (green pepper, 9,10) rice, 11–13) and potato, 14,15) but these plants generally have few bioactive compounds. Herbs contain various secondary metabolites that may have synergistic effects with the vaccine.…”

Section: Introductionmentioning

confidence: 99%

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Production of the herb <i>Ruta chalepensis</i> L. expressing amyloid β-GFP fusion protein

Yoshida¹,

Watanabe

Ishiura

2019

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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The herb Ruta chalepensis L. exhibits medical effects, such as anti-inflammatory, central nervous system depressant, and antipyretic activities. However, a genetic transformation method has not yet been developed for this species. In this paper, a simple and efficient tissue culture and genetic transformation system for R. chalepensis is reported. An amyloid β-peptide (Aβ) gene, which is considered to be a causative agent of Alzheimer’s disease (AD), fused with green-fluorescent protein (GFP), was introduced into R. chalepensis. When the leaves of R. chalepensis expressing Aβ-GFP were administered orally to C57BL/6J mice, serum anti-Aβ antibody titers of several mice were elevated without the use of an adjuvant. These results indicated that an oral vaccine against AD using R. chalepensis may be feasible. R. chalepensis is rich in bioactive compounds that may have synergistic effects with the vaccine for AD. Plant-derived vaccines are safer and cheaper than those produced from animal cells or microbes, because plants can serve as biofactories at low cost and with high biosynthetic capacity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Production of the herb <i>Ruta chalepensis</i> L. expressing amyloid β-GFP fusion protein

Yoshida¹,

Watanabe

Ishiura

2019

Proceedings of the Japan Academy. Ser. B: Physical and Biologic

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“…Nevertheless, when CTB was employed as an adjuvant, additional rice seed protein antibodies were detected, indicating that this vaccine may induce food allergies. As a consequence, in order to verify the vaccine’s safety, serum IgG1 and IgG2a isotypes of anti-seed protein antibodies were examined to evaluate possible inflammatory reactions, and it was observed that Aβ fed mice generated a non-inflammatory Th2 response [ 93 ]. Nonetheless, the authors stated that the production of rice seed protein antibodies might be suppressed when immunological tolerance is induced by constant rice consumption.…”

Section: Progress On Plant-based Vaccines For Alzheimer’s Diseasementioning

confidence: 99%

Current Advances of Plant-Based Vaccines for Neurodegenerative Diseases

et al. 2023

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Neurodegenerative diseases (NDDs) are characterized by the progressive degeneration and/or loss of neurons belonging to the central nervous system, and represent one of the major global health issues. Therefore, a number of immunotherapeutic approaches targeting the non-functional or toxic proteins that induce neurodegeneration in NDDs have been designed in the last decades. In this context, due to unprecedented advances in genetic engineering techniques and molecular farming technology, pioneering plant-based immunogenic antigen expression systems have been developed aiming to offer reliable alternatives to deal with important NDDs, including Alzheimer’s disease, Parkinson’s disease, and multiple sclerosis. Diverse reports have evidenced that plant-made vaccines trigger significant immune responses in model animals, supported by the production of antibodies against the aberrant proteins expressed in the aforementioned NDDs. Moreover, these immunogenic tools have various advantages that make them a viable alternative for preventing and treating NDDs, such as high scalability, no risk of contamination with human pathogens, cold chain free production, and lower production costs. Hence, this article presents an overview of the current progress on plant-manufactured vaccines for NDDs and discusses its future prospects.

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