Plant-Based Vaccines as a Global Vaccination Approach: Current Perspectives

Govea‐Alonso, Dania O.; Rybicki, Edward P.; Rosales‐Mendoza, Sergio

doi:10.1007/978-1-4939-0850-9_13

Cited by 9 publications

(5 citation statements)

References 52 publications

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“…The ability of the plant expression system to produce diagnostic reagents, vaccine antigens, and monoclonal antibodies is well-documented [70][71][72][73][74][75][76][77][78][79][80]. Our group has recently developed a plant-produced subunit vaccine containing SARS-CoV-2 RBD domain fused with the Fc region of human IgG (SARS-CoV-2 RBD-Fc).…”

Section: Discussionmentioning

confidence: 99%

Immunogenicity Studies of Plant-Produced SARS-CoV-2 Receptor Binding Domain-Based Subunit Vaccine Candidate with Different Adjuvant Formulations

Siriwattananon

Manopwisedjaroen

Shanmugaraj³

et al. 2021

Vaccines

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Due to the rapid transmission of the coronavirus disease 2019 (COVID-19) causing serious public health problems and economic burden, the development of effective vaccines is a high priority for controlling the virus spread. Our group has previously demonstrated that the plant-produced receptor-binding domain (RBD) of SARS-CoV-2 fused with Fc of human IgG was capable of eliciting potent neutralizing antibody and cellular immune responses in animal studies, and the immunogenicity could be improved by the addition of an alum adjuvant. Here, we performed a head-to-head comparison of different commercially available adjuvants, including aluminum hydroxide gel (alum), AddaVax (MF59), monophosphoryl lipid A from Salmonella minnesota R595 (mPLA-SM), and polyinosinic-polycytidylic acid (poly(I:C)), in mice by combining them with plant-produced RBD-Fc, and the differences in the immunogenicity of RBD-Fc with different adjuvants were evaluated. The specific antibody responses in terms of total IgG, IgG1, and IgG2a subtypes and neutralizing antibodies, as well as vaccine-specific T-lymphocyte responses, induced by the different tested adjuvants were compared. We observed that all adjuvants tested here induced a high level of total IgG and neutralizing antibodies, but mPLA-SM and poly (I:C) showed the induction of a balanced IgG1 and IgG2a (Th2/Th1) immune response. Further, poly (I:C) significantly increased the frequency of IFN-γ-expressing cells compared with control, whereas no significant difference was observed between the adjuvanted groups. This data revealed the adjuvants’ role in enhancing the immune response of RBD-Fc vaccination and the immune profiles elicited by different adjuvants, which could prove helpful for the rational development of next-generation SARS-CoV-2 RBD-Fc subunit vaccines. However, additional research is essential to further investigate the efficacy and safety of this vaccine formulation before clinical trials.

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Section: Discussionmentioning

confidence: 99%

Immunogenicity Studies of Plant-Produced SARS-CoV-2 Receptor Binding Domain-Based Subunit Vaccine Candidate with Different Adjuvant Formulations

Siriwattananon

Manopwisedjaroen

Shanmugaraj³

et al. 2021

Vaccines

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“…Plants are a suitable platform to use during an emergency situation because, in comparison to other expression systems, it has many advantages, such as cost, safety, and speed of production, and it has been largely used for producing biopharmaceuticals over the past decade [20,[30][31][32][33]. Many research groups have evaluated and thoroughly studied the feasibility of plant-derived biopharmaceuticals and vaccines [34,35]. In particular, plants are used as an expression system for antibodies, vaccines, diagnostic reagents, human serum albumin, cytokines, and growth factors production [36].…”

Section: Discussionmentioning

confidence: 99%

Plant-Produced S1 Subunit Protein of SARS-CoV-2 Elicits Immunogenic Responses in Mice

et al. 2022

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SARS-CoV-2 is responsible for the ongoing COVID-19 pandemic. The virus spreads rapidly with a high transmission rate among humans, and hence virus management has been challenging owing to finding specific therapies or vaccinations. Hence, an effective, low-cost vaccine is urgently required. In this study, the immunogenicity of the plant-produced S1 subunit protein of SARS-CoV-2 was examined in order to assess it as a potential candidate for SARS-CoV-2. The SARS-CoV-2 S1-Fc fusion protein was transiently produced in Nicotiana benthamiana. Within four days of infiltration, the SARS-CoV-2 S1-Fc protein was expressed in high quantities, and using protein A affinity column chromatography, plant-produced S1-Fc protein was purified from the crude extracts. The characterization of plant-produced S1-Fc protein was analyzed by SDS-PAGE and Western blotting. Immunogenicity of the purified S1-Fc protein formulated with alum induced both RBD specific antibodies and T cell immune responses in mice. These preliminary results indicated that the plant-produced S1 protein is immunogenic in mice.

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“…The plant-made vaccines or therapeutic products can be easily stored or lyophilized for longer shelf life without the requirement of low temperatures for keeping them stable and retaining their activity [ 110 ]. Further, the pharmaceutically-relevant proteins produced in plants are considered to be safer when compared to bacterial or mammalian cells as the risk of contamination during the manufacturing processes is low and the do not present serious bio-safety threats [ 111 ]. The major advantage of this expression system is the ability to perform post-translational modifications which are likely crucial for protein folding and the biological function of AMP molecules [ 112 , 113 ].…”

Section: Plant Molecular Farmingmentioning

confidence: 99%

Biotechnological Insights on the Expression and Production of Antimicrobial Peptides in Plants

Shanmugaraj¹,

Bulaon

Malla³

et al. 2021

Molecules

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The emergence of drug-resistant pathogens poses a serious critical threat to global public health and requires immediate action. Antimicrobial peptides (AMPs) are a class of short peptides ubiquitously found in all living forms, including plants, insects, mammals, microorganisms and play a significant role in host innate immune system. These peptides are considered as promising candidates to treat microbial infections due to its distinct advantages over conventional antibiotics. Given their potent broad spectrum of antimicrobial action, several AMPs are currently being evaluated in preclinical/clinical trials. However, large quantities of highly purified AMPs are vital for basic research and clinical settings which is still a major bottleneck hindering its application. This can be overcome by genetic engineering approaches to produce sufficient amount of diverse peptides in heterologous host systems. Recently plants are considered as potential alternatives to conventional protein production systems such as microbial and mammalian platforms due to their unique advantages such as rapidity, scalability and safety. In addition, AMPs can also be utilized for development of novel approaches for plant protection thereby increasing the crop yield. Hence, in order to provide a spotlight for the expression of AMP in plants for both clinical or agricultural use, the present review presents the importance of AMPs and efforts aimed at producing recombinant AMPs in plants for molecular farming and plant protection so far.

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Plant-Based Vaccines as a Global Vaccination Approach: Current Perspectives

Cited by 9 publications

References 52 publications

Immunogenicity Studies of Plant-Produced SARS-CoV-2 Receptor Binding Domain-Based Subunit Vaccine Candidate with Different Adjuvant Formulations

Immunogenicity Studies of Plant-Produced SARS-CoV-2 Receptor Binding Domain-Based Subunit Vaccine Candidate with Different Adjuvant Formulations

Plant-Produced S1 Subunit Protein of SARS-CoV-2 Elicits Immunogenic Responses in Mice

Biotechnological Insights on the Expression and Production of Antimicrobial Peptides in Plants

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