Production of an Allelopathic Polyacetylene in Hairy Root Cultures of Goldenrod (<i>Solidago altissima</i>L.)

Inoguchi, Masahiko; Ogawa, Satoshi; Furukawa, Shoei; Kondo, Hirokiyo

doi:10.1271/bbb.67.863

Cited by 30 publications

(11 citation statements)

References 25 publications

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“…Some possible reasons for an increased rate of transformation could be the effect of PO 4 starvation in activating vir G expression (Winans 1990), the formation of biofilm (Danhorn et al 2004), the possibility of a Ca +2 inhibitory effect on the expression of virulence genes of bacteria (Flego et al 1997), and increased proliferation of Agrobacterium in the medium which is lacking some elements (Azadi et al 2010 The high rate of root formation obtained in low strength MS salt medium in Solidago altissima has also been reported (Inoguchi et al 2003). More studies on the role of these elements are needed to discover how T-DNA transfer is regulated by mineral compounds.…”

Section: Resultsmentioning

confidence: 93%

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Sharafi

Sohi

Azadi

et al. 2014

Physiol Mol Biol Plants

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An efficient hairy root induction system for an important endangered medicinal plant, Dracocephalum kotschyi, was developed through Agrobacterium rhizogenesmediated transformation by modifying the co-cultivation medium using five bacterial strains, A4, ATCC15834, LBA9402, MSU440, and A13 (MAFF-02-10266). A drastic increase in transformation frequency was observed when a Murashige and Skoog medium lacking NH 4 NO 3 KH 2 PO 4, KNO 3 and CaCl 2 was used, resulting in hairy root induction frequencies of 52.3 %, 69.6 %, 48.6 %, 89.0 %, and 80.0 % by A4, A13, LBA9402, MSU440, and ATCC15834 strains, respectively. For shoot induction, hairy roots and unorganized tumors induced by strain ATCC15834 were placed on an MS media supplemented with 0.1, 0.25, 0.5, and 1 mg/l BA plus 0.1 mg/l NAA. The high frequency of shoot regeneration and number of shoot were obtained in the medium containing 0.25 mg/l BA and 0.1 mg/l NAA. Root induction occurred from the base of regenerated shoots on the MS medium supplemented with 0.5 mg/l IBA after 10 days.

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Section: Resultsmentioning

confidence: 93%

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Sharafi

Sohi

Azadi

et al. 2014

Physiol Mol Biol Plants

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“…According to these authors, differences in the content of polyacetylenes could possibly be caused by storage of plant material before analysis and not necessarily by (Hansen and Boll 1986). Although falcarinol has not been identified in L. officinale roots in planta, polyacetylenes are known to be produced by roots of other Apiaceae (Lam et al 1992), and have been found in essential oils isolated from other hairy roots (Inoguchi et al 2003).…”

Section: Resultsmentioning

confidence: 99%

Nitrogen stress induction on Levisticum officinale hairy roots grown in darkness and under photoperiod conditions: effect on growth and volatile components

et al. 2008

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Six-year-old Levisticum officinale (lovage) hairy root cultures were used to study the effect of eight different NH(4) (+):NO(3) (-) ratios on their growth and volatile components. All cultures were kept at 24 degrees C on orbital shakers at 80 rpm, in darkness or in a 16 h light/8 h dark photoperiod. Growth was evaluated by dry and fresh weight determination. The volatiles were isolated by distillation-extraction and analysed by GC and GC-MS. Greater growth was attained in darkness with 10:90 (control, SH medium), 50:50 and 25:75 NH(4) (+):NO(3) (-) ratios, and also with SH control medium under the photoperiod condition, with a 10, 14, 12.5 and 12.5 fold increase of biomass in terms of dry weight, respectively, at the end of 42 days of growth. UPGMA cluster analysis of the mixtures of volatiles isolated from the hairy roots grown with different NH(4) (+):NO(3) (-) ratios confirmed their chemical variability. Although no particular grouping was detected in relation to the NH(4) (+):NO(3) (-) ratios or light conditions studied, most of the mixtures of volatiles isolated from the hairy roots were either dominated by n-octanal, (Z)-falcarinol or both components in about the same relative amounts.

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“…1) under dark conditions, which was 2-3 times the production from untransformed roots [270]. While the greenish roots cultured under illumination grew more rapidly, which was presumed to be due to hemiautotrophy, the production of the polyacetylenes was consistently lower.…”

Section: Use Of Higher Plant Cell Cultures To Study Polyacetylene Biomentioning

confidence: 90%

Biosynthesis and function of polyacetylenes and allied natural products

Minto

Blacklock

2008

Progress in Lipid Research

288

228

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Polyacetylenic natural products are a substantial class of often unstable compounds containing a unique carbon-carbon triple bond functionality, that are intriguing for their wide variety of biochemical and ecological functions, economic potential, and surprising mode of biosynthesis. Isotopic tracer experiments between 1960 and 1990 demonstrated that the majority of these compounds are derived from fatty acid and polyketide precursors. During the past decade, research into the metabolism of polyacetylenes has swiftly advanced, driven by the cloning of the first genes responsible for polyacetylene biosynthesis in plants, moss, fungi, and actinomycetes, and the initial characterization of the gene products.The current state of knowledge of the biochemistry and molecular genetics of polyacetylenic secondary metabolic pathways will be presented together with an up-to-date survey of new terrestrial and marine natural products, their known biological activities, and a discussion of their likely metabolic origins.

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Production of an Allelopathic Polyacetylene in Hairy Root Cultures of Goldenrod (Solidago altissimaL.)

Cited by 30 publications

References 25 publications

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Nitrogen stress induction on Levisticum officinale hairy roots grown in darkness and under photoperiod conditions: effect on growth and volatile components

Biosynthesis and function of polyacetylenes and allied natural products

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