Nitrogen stress induction on Levisticum officinale hairy roots grown in darkness and under photoperiod conditions: effect on growth and volatile components

Costa, Monya M.; Figueiredo, A. Cristina; Barroso, José G.; Pedro, Luís G.; Deans, Stanley G.; Scheffer, J. J. C.

doi:10.1007/s10529-008-9664-y

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…The production of this compound by the hairy roots and not by the plant roots may reflect a stress defence mechanism induced by the in vitro growth conditions [18]. The differences seen in the present analysis when compared to the previous study on the constitutive volatiles of L. officinale hairy roots maintained in SH medium [13], such as higher n-octanal levels and the much lower β-phellandrene levels, can be partly explained by the fact that these are biological systems and, as mentioned by Costa et al [17], the use of different developmental hairy root stages and/or the type of inoculum (richer in meristematic zones, such as lateral branching roots) may explain slight differences in the growth of the roots that will be reflected in their secondary metabolite production capacity. Independently of the added substrate, be it geraniol or menthol, the constitutive volatiles of L. officinale hairy roots were always detected in variable amounts, together with the substrates and the corresponding biotransformation products, when produced.…”

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confidence: 68%

“…1B. Despite some minor variations in growth due to possible different inocula developmental stages, the outline of the growth curves was identical to those previously obtained for hairy roots of the same species [13], [17]. Forty-one compounds were identified in the oils isolated from the control lovage hairy roots (constitutive components), which were maintained in SH medium in darkness for seven weeks.…”

supporting

confidence: 67%

“…, palmitic acid (3 -10 %), and (Z)-falcarinol (1 -45 %) were the main volatile components -i. e., those that were detected in relative amounts > 5% , Table 1S. All these compounds had previously been detected in lovage hairy roots [13], [17], although falcarinol was not detected in the plant roots [13]. The production of this compound by the hairy roots and not by the plant roots may reflect a stress defence mechanism induced by the in vitro growth conditions [18].…”

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confidence: 85%

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Menthol and Geraniol Biotransformation and Glycosylation Capacity ofLevisticum officinaleHairy Roots

et al. 2009

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The biotransformation capacity of Levisticum officinale W.D.J. Koch hairy root cultures was studied by evaluating the effect of the addition of 25 mg/L menthol or geraniol on morphology, growth, and volatiles production. L. officinale hairy root cultures were maintained for 7 weeks in SH medium, in darkness at 24 degrees C and 80 r.p.m., and the substrates were added 15 days after inoculation. Growth was evaluated by measuring fresh and dry weight and by using the dissimilation method. Volatiles composition was analyzed by GC and GC-MS. Hairy roots morphology and growth were not influenced by substrate addition. No new volatiles were detected after menthol addition and, as was also the case with the control cultures, volatiles of these hairy roots were dominated by (Z)-falcarinol (1-45%), N-octanal (3-8%), palmitic acid (3-10%), and (Z)-ligustilide (2-9%). The addition of geraniol induced the production of six new volatiles: nerol/citronellol/neral (traces-15%), alpha-terpineol (0.2-3%), linalool (0.1-1.2%), and geranyl acetate (traces-2%). The relative amounts of the substrates and some of their biotransformation products decreased during the course of the experiment. Following the addition of beta-glycosidase to the remaining distillation water, analysis of the extracted volatiles showed that lovage hairy roots were able to convert both substrates and their biotransformation products into glycosidic forms. GC:gas chromatography GC-MS:gas chromatography-mass spectrometry SH:Schenk and Hildebrandt (1972) culture medium.

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confidence: 67%

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confidence: 85%

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Menthol and Geraniol Biotransformation and Glycosylation Capacity ofLevisticum officinaleHairy Roots

et al. 2009

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“…These results are in agreement with those obtained previously by Santos et al (2002) with the same in vitro system, suggesting that A. graveolens hairy roots have a relatively stable production of volatile compounds, as the cultures have been maintained for over twelve years with a routine subculture every three weeks. The higher relative amount of fatty acids, found in the present study, when compared to that obtained by Santos et al (2002), may be due to the different culture medium used, which may alter the volatile composition as was found in L. officinale hairy roots (Costa et al 2008). …”

Section: Constitutive Volatile Componentscontrasting

confidence: 51%

Biotransformation of menthol and geraniol by hairy root cultures of Anethum graveolens: effect on growth and volatile components

et al. 2009

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Two oxygen-containing monoterpene substrates, menthol or geraniol (25 mg l(-1)), were added to Anethum graveolens hairy root cultures to evaluate the influence of the biotransformation capacity on growth and production of volatile compounds. Growth was assessed by the dissimilation method and by fresh and dry weight measurement. The volatiles were analyzed by GC and GC-MS. The total constitutive volatile component was composed, in more than 50%, by falcarinol (17-52%), apiole (11-24%), palmitic acid (7-16%), linoleic acid (4-9%), myristicin (4-8%) and n-octanal (2-5%). Substrate addition had no negative influence on growth. The relative amount of menthol quickly decreased 48 h after addition, and the biotransformation product menthyl acetate was concomitantly formed. Likewise, the added geraniol quickly decreased over 48 h alongside with the production of the biotransformation products. The added geraniol was biotransformed in 10 new products, the alcohols linalool, alpha-terpineol and citronellol, the aldehydes neral and geranial, the esters citronellyl, neryl and geranyl acetates and linalool and nerol oxides.

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Efficiency of different Agrobacterium rhizogenes strains on hairy roots induction in Solanum mammosum

Ooi

Ahmad

Stanslas

et al. 2012

World J Microbiol Biotechnol

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This article presents the abilities and efficiencies of five different strains of Agrobacterium rhizogenes (strain ATCC 31798, ATCC 43057, AR12, A4 and A13) to induce hairy roots on Solanum mammosum through genetic transformation. There is significant difference in the transformation efficiency (average number of days of hairy root induction) and transformation frequency for all strains of A. rhizogenes (P < 0.05). Both A. rhizogenes strain AR12 and A13 were able to induce hairy root at 6 days of co-cultivation, which were the fastest among those tested. However, the transformation frequencies of all five strains were below 30 %, with A. rhizogenes strain A4 and A13 showing the highest, which were 21.41 ± 10.60 % and 21.43 ± 8.13 % respectively. Subsequently, the cultures for five different hairy root lines generated by five different strains of bacteria were established. However, different hairy root lines showed different growth index under the same culture condition, with the hairy root lines induced by A. rhizogenes strain ATCC 31798 exhibited largest increase in fresh biomass at 45 days of culture under 16 h light/8 h dark photoperiod in half-strength MS medium. The slowest growing hairy root line, which was previously induced by A. rhizogenes strain A13, when cultured in optimized half-strength MS medium containing 1.5 times the standard amount of ammonium nitrate and potassium nitrate and 5 % (w/v) sucrose, had exhibited improvement in growth index, that is, the fresh biomass was almost double as compared to its initial growth in unmodified half-strength MS medium.

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Nitrogen stress induction on Levisticum officinale hairy roots grown in darkness and under photoperiod conditions: effect on growth and volatile components

Cited by 7 publications

References 12 publications

Menthol and Geraniol Biotransformation and Glycosylation Capacity ofLevisticum officinaleHairy Roots

Menthol and Geraniol Biotransformation and Glycosylation Capacity ofLevisticum officinaleHairy Roots

Biotransformation of menthol and geraniol by hairy root cultures of Anethum graveolens: effect on growth and volatile components

Efficiency of different Agrobacterium rhizogenes strains on hairy roots induction in Solanum mammosum

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