Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Sharafi, Ali; Sohi, Haleh Hashemi; Azadi, Pejman; Sharafi, Ata Allah

doi:10.1007/s12298-013-0217-z

Cited by 45 publications

(22 citation statements)

References 24 publications

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“…Similar results have been drawn in another study on the bottle gourd that explains BA is essential for shoot bud formation (Saha et al 2007). In our experiments, shoot formation was not detected on the hormone-free medium, which is in consistence with the results obtained by Pogostemon cablin and Dracocephalum kotschyi (He-Ping et al 2011;Sharafi et al 2014), respectively. In summary, the insertion of native A. rhizogenes T-DNA into the O. vulgare genome worked successfully.…”

Section: Molecular Analysis Of Transgenic Hairy Rootssupporting

confidence: 92%

Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare

Habibi

Silva

et al. 2016

Physiol Mol Biol Plants

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Origanum vulgare L is commonly known as a wild marjoram and winter sweet which has been used in the traditional medicine due to its therapeutic effects as stimulant, anticancer, antioxidant, antibacterial, anti-inflammatory and many other diseases. A reliable gene transfer system via Agrobacterium rhizogenes and plant regeneration via hairy roots was established in O. vulgare for the first time. The frequency of induced hairy roots was different by modification of the co-cultivation medium elements after infection by Agrobacterium rhizogenes strains K599 and ATCC15834. ). The frequency of shoot generation (85.18 %) was achieved in medium fortified with 0.25 mg/L −1 of BA. Shoots were placed on MS medium with 0.25 mg/l IBA for root induction. Roots appeared and induction rate was achieved after 15 days.

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Section: Molecular Analysis Of Transgenic Hairy Rootssupporting

confidence: 92%

Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare

Habibi

Silva

et al. 2016

Physiol Mol Biol Plants

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“…Prior studies indicated that vir G expression can be triggered by low levels of PO 4 and proposed that PO 4 deficiency could be a helpful signal to prompt genetic transformation. Similar results were described in transformation by A. tumefaciens in Lilium (36) and genetically transformed root generation of Papaver bracteatum, Nepeta pogonosperma, Dracocephalum kotschyi, and Artemisia aucheri (26,31,34,37). 4 Jundishapur J Nat Pharm Prod.…”

Section: Discussionsupporting

confidence: 78%

“…Sharafi et al (2014a) used liquid inoculation and solid co-cultivation media in absence of macro elements, such as KH 2 PO 4 , NH 4 NO 3 , KNO 3 and CaCl 2 , to transform medicinal plant Dracocephalum kotschyi with high efficiency (31). In another study, Henzi et al (2000) indicated that the usage of acetosyringone and arginine in the agar solidified co-cultivation medium, which revealed major enhancement in transformation rate of Brassica by Agrobacterium (32).…”

Section: Introductionmentioning

confidence: 99%

Genetically Transformed Root-Based Culture Technology in Medicinal Plant Cosmos bipinnatus

Jaberi¹,

Sharafi²,

Sharafi³

et al. 2018

Jundishapur J Nat Pharm Prod

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Background: Cosmos bipinnatus is an important medicinal plant with antioxidative, antigenotoxic, anti-inflammatory, and antiproliferative effects on several cancer cell lines. It has great potential for development as a promising cancer chemo-preventive agent. Hairy root-based culture technology is a new sustainable production platform for producing specific pharmaceutical secondary metabolites. Objectives: The current study developed and introduced a reliable transformation system for C. bipinnatus by optimization of aspects important in transformation frequency using Agrobacterium rhizogenes. Methods: Five bacterial strains, including ATCC 15834, ATCC 31798, A7, MAFF-02-10266, and MSU440, 2 explant types (leaf and stem), and 2 co-cultivation media (full MS and ½ MS) were examined. Genomic DNA was extracted using a modified CTAB protocol from putative transgenic root lines and the control root. Transgenic hairy root lines were approved by means of Polymerase Chain Reaction (PCR) using specific rolB gene primers. Results:The highest ratio of genetically transformed root induction was found from leaf explants using A. rhisogenes strains ATCC15834 and MSU440 (72% to 73%). When ½ MS medium was used as a co-cultivation medium, a significant increase in transformation frequency (84%) was observed. Conclusions: The MSU440 Agrobacterium strain and ½ MS co-cultivation medium could significantly improve genetic transformation efficiency for establishment of hairy root-based cultures for C. bipinnatus.

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“…Transformation efficiency can be over 40 % using binary vector (co-transformation of rol gene and target gene) by this protocol. The transgenic hairy roots can be used as explants for plantlet regeneration to obtain stable transgenic plants (Sharafi et al 2013a). …”

Section: Discussionmentioning

confidence: 99%

A reliable and efficient protocol for inducing genetically transformed roots in medicinal plant Nepeta pogonosperma

Valimehr

Sanjarian

Sohi

et al. 2014

Physiol Mol Biol Plants

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Nepeta pogonosperma is an important medicinal plant with anti-inflammatory effects. An efficient and reliable transformation system for this plant was developed through optimization of several factors which affected the rate of Agrobacterium rhizogenes mediated transformation. Five bacterial strains, A4, ATCC15834, LBA9402, MSU440 and A13, two explant types, leaves and stems, and several co-cultivation media were examined. The maximum rate of hairy root induction was obtained from stem explants using MSU440 and ATCC15834 bacterial strains. A drastic increase in the frequency of transformation (91 %) was observed when MS medium lacking NH 4 NO 3 , KH 2 PO 4 , KNO 3 and CaCl 2 . Hairy root lines were confirmed by polymerase chain reaction (PCR) using primers of the rolB gene. According to Southern blot analysis, one T-DNA copy was inserted into each of the hairy root lines. In the present study, transgenic hairy roots have been obtained trough genetic transformation by A. rhizogenes harbouring two plasmids, the Ri plasmid and pBI121 binary vector harbouring gus reporter gene. Expression of the gus gene in transgenic hairy root was confirmed by histochemical GUS assay.

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Hairy root induction and plant regeneration of medicinal plant Dracocephalum kotschyi

Cited by 45 publications

References 24 publications

Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare

Efficient genetic transformation and regeneration system from hairy root of Origanum vulgare

Genetically Transformed Root-Based Culture Technology in Medicinal Plant Cosmos bipinnatus

A reliable and efficient protocol for inducing genetically transformed roots in medicinal plant Nepeta pogonosperma

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