Genetically Transformed Root-Based Culture Technology in Medicinal Plant Cosmos bipinnatus

Jaberi, Mehdi; Sharafi, Ali; Sharafi, Ata Allah; Azadi, Pejman; Kheiri-Manjili, Hamidreza; Danafar, Hossein; Ahmadnia, Alireza

doi:10.5812/jjnpp.67182

Cited by 6 publications

(2 citation statements)

References 37 publications

(52 reference statements)

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“…Hairy root culture technology has mostly been used as a sustainable production platform for producing specific pharmaceutical secondary metabolites and in the evaluation of key genes involved in valuable medicinal metabolite pathway studies. It has been possible to increase the biosynthesis of bioactive secondary metabolites through induction of defense‐related gene expression . Genetically transformed root culture, as a green factory, has many advantages such as fast growth in the absence of growth regulators in culture media and the production of high‐value phytochemicals.…”

Section: Introductionmentioning

confidence: 99%

“…It has been possible to increase the biosynthesis of bioactive secondary metabolites through induction of defense-related gene expression. 21,22 Genetically transformed root culture, as a green factory, has many advantages such as fast growth in the absence of growth regulators in culture media and the production of high-value phytochemicals. Secondary metabolic engineering through the manipulation of the key genes responsible for secondary metabolic biosynthesis might aid the induction of the accumulation of pharmaceutical compounds in hairy root cultures.…”

Section: Introductionmentioning

confidence: 99%

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Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

et al. 2019

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BACKGROUND Dracocephalum kotschyi Boiss. is a valuable source of rosmarinic acid (RA) and methoxylated hydroxyflavones (such as xanthomicrol and cirsimaritin) with antioxidative and antiplatelet effects and with antiproliferative potential against various cancer cells. The extensive application of nanotechnology in hairy root cultures is a new sustainable production platform for producing these active constituents. In the present study, hairy roots derived from 4‐week‐old leaves and Agrobacterium rhizogenes strain ATCC15834 were used to investigate the impact of various concentrations of iron oxide nanoparticles (Fe NPs) in two elicitation time exposures (24 and 48 h) on growth, antioxidant enzyme activity, total phenolic and flavonoid content (TPC and TFC), and some polyphenols. Gene expression levels of phenylalanine ammonia‐lyase (pal) and rosmarinic acid synthase (ras) were also analyzed. RESULTS Iron nanoparticles enhanced biomass accumulation in hairy roots. The treatment time and Fe NP dosage largely improved the activity of antioxidant enzymes, TPC and TFC. The highest RA (1194 μg g−1 FW) content (9.7‐fold), compared to controls, was detected with 24 h of exposure to 75 mg L−1 Fe NP, which was consistent with the expression of pal and ras genes under the influence of elicitation. The xanthomicrol, cirsimaritin, and isokaempferide content was increased 11.87, 3.85, and 2.27‐fold, respectively. CONCLUSION Stimulation of D. kotschyi hairy roots by Fe NPs led to a significant increase in the induction and production of important pharmaceutical compounds such as rosmarinic acid and xanthomicrol. © 2019 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

et al. 2019

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Improvement of sulforaphane production in hairy root cultures of broccoli (Brassica oleracea L. var. italica) by eliciting Myrosinase gene expression and its effect on breast cancer cells

Amer,

Mohamed,

Rahman

et al. 2024

Plant Cell Tiss Organ Cult

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Plant-derived hairy root cultures have the potential to be a significant biotechnological tool for producing valuable secondary metabolite compounds. This study deals with the production of broccoli’s hairy root cultures via the mediation of Agrobacterium rhizogenes and investigates the effects of different concentrations of copper sulfate (CuSO4) and zinc sulfate (ZnSO4) as two elicitors on the production of sulforaphane in broccoli's hairy root. The expression of the Myrosinase (MY) gene, associated with sulforaphane production, was quantified using real-time PCR. Moreover, the cytotoxic effect of different treatments of sulforaphane on the breast cancer cell line, MDA-MB-231, was estimated and the transcription levels of apoptosis-associated genes were also determined. The highest amount of sulforaphane was produced at 4 µM CuSO4 after 16 h and 16 µM ZnSO4 after 8 h of elicitation. Overexpression of the MY gene was found to be associated with increased production of sulforaphane. All sulforaphane treatments induced a dose-dependent reduction in the viability of cancer cells. The sulforaphane extracted from broccoli's hairy roots treated with CuSO4 exerted a higher cytotoxic effect on the MDA-MB-231 breast cancer cell line than the sulforaphane extracted from broccoli's hairy root treated with ZnSO4. All treatments of sulforaphane up-regulated the Apoptosis Regulator (Bax) gene, Proapoptotic Bcl2 Associated X, Caspase-3, Caspase-8, and Caspase-9, while down-regulating the B-cell lymphoma 2 (Bcl-2) gene (antiapoptotic) transcription. The overall results showed an antiapoptotic effect of sulforaphane extract derived from broccoli's hairy roots on MDAMB-231 breast cancer cells, thereby establishing the potential anticancer activity of the sulforaphane produced in broccoli's hairy root cultures.

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Enhancing the aervine and methylaervine production in in vitro cultures of Aerva javanica (Burm. F.) Schult via elicitors and Agrobacterium rhizogenes-mediated hairy root cultures

Boobalan,

Srinivasan,

Keerthanaa

et al. 2024

J. Plant Biochem. Biotechnol.

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Genetically Transformed Root-Based Culture Technology in Medicinal Plant Cosmos bipinnatus

Cited by 6 publications

References 37 publications

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

Iron oxide nanoparticles: a novel elicitor to enhance anticancer flavonoid production and gene expression in Dracocephalum kotschyi hairy‐root cultures

Improvement of sulforaphane production in hairy root cultures of broccoli (Brassica oleracea L. var. italica) by eliciting Myrosinase gene expression and its effect on breast cancer cells

Enhancing the aervine and methylaervine production in in vitro cultures of Aerva javanica (Burm. F.) Schult via elicitors and Agrobacterium rhizogenes-mediated hairy root cultures

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