Improvement of sulforaphane production in hairy root cultures of broccoli (Brassica oleracea L. var. italica) by eliciting Myrosinase gene expression and its effect on breast cancer cells
May Ahmed Amer,
Thoria Rashad Mohamed,
Raoufa A. Abdel Rahman
et al.
Abstract:Plant-derived hairy root cultures have the potential to be a significant biotechnological tool for producing valuable secondary metabolite compounds. This study deals with the production of broccoli’s hairy root cultures via the mediation of Agrobacterium rhizogenes and investigates the effects of different concentrations of copper sulfate (CuSO4) and zinc sulfate (ZnSO4) as two elicitors on the production of sulforaphane in broccoli's hairy root. The expression of the Myrosinase (MY) gene, associated with sul… Show more
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