Production of allitol from D‐psicose by a novel isolated strain of Klebsiella oxytoca G4A4

Abstract

A novel bacterium capable of producing allitol from D-psicose was isolated from soil and identified as Klebsiella oxytoca G4A4. An efficient method for the transformation of D-psicose to allitol was achieved through the resting cell reaction. Ribitol as an inducer is suitable for cell cultivation, and cells are most active in Tris-HCl buffer (pH 8.0) at 37 °C with a density of 40 (OD600 nm ). After the reaction, the final conversion rates of the washed cells were approximately 87, 83, and 55% at D-psicose concentrations of 0.25, 0.5, and 1%, respectively. The product from D-psicose was purified and determined to be allitol by high-performance liquid chromatography and nuclear magnetic resonance spectroscopy.

Cloning and characterization of a new ribitol dehydrogenase fromProvidencia alcalifaciensRIMD 1656011

“…Allitol is a six-carbon alcohol that can be used as a low-calorie sweetener and as a laxative for treatment of constipation. 3 -6 RDH has previously been produced from Aerobacter aerogenes, 7 Enterobacter agglomerans, 8 Gluconobacter oxydans, 4 Klebsiella aerogenes, 9 Rhodobacter sphaeroides, 10 Klebsiella oxytoca 11 and Zymomonas mobilis. 5 In a previous study the cloning, expression and characterization of a new RDH from Z. mobilis was reported as the first characterized recombinant RDH.…”

Recent advances in d -allulose: Physiological functionalities, applications, and biological production

“…Moreover, D-allulose could be spray-dried by the addition of excipients, which would be helpful for pulmonary drug delivery (Kawakami et al, 2013;Kawakami et al, 2014). Additionally, it is reported that D-allulose is the precursor of other hexoses and plays an extremely important role in the production of D-allose (Feng, Mu, & Jiang, 2013;Yeom, Seo, Kim, & Oh, 2011), D-allitol (Han et al, 2014), D-talitol (Sasahara, Mine, & Izumori, 1998), D-tagatose (Yoshihara, Shinohara, Hirotsu, & Izumori, 2006) and D-altrose (Menavuvu et al, 2006).…”

Allitol: production, properties and applications

“…The Michaelis-Menten constants (K m ) of RDHs from K. aerogenes, Rhodobacter sphaeroides, E. agglomerans, G. oxydans, Z. mobilis, E. aerogenes and P. alcalifaciens with ribitol were 8, 6.3, 32.2, 1.2, 11.8, 10.3 and 13.9 mM, respectively (Kahle et al, 1992;Muniruzzaman et al, 1995b;Adachi et al, 2001;Moon et al, 2010;Han et al, 2014;Hassanin et al, 2015;Singh et al, 2015). No previous study has examined the kinetic parameters of reduction in D-psicose into allitol by RDH.…”

“…Use of this strain for production of allitol did not involve consumption of the substrate (D-psicose) (Muniruzzaman et al, 1995a). Han et al (2014) reported production of allitol from D-psicose using Klebsiella oxytoca G4A4 isolated from soil. Transformation was investigated using a resting cell reaction.…”

“…However, the kinetic parameters of RDH with ribitol in the presence of the coenzyme NAD + have been reported. The Michaelis-Menten constants (K m ) of RDHs from K. aerogenes, Rhodobacter sphaeroides, E. agglomerans, G. oxydans, Z. mobilis, E. aerogenes and P. alcalifaciens with ribitol were 8, 6.3, 32.2, 1.2, 11.8, 10.3 and 13.9 mM, respectively (Kahle et al, 1992;Muniruzzaman et al, 1995b;Adachi et al, 2001;Moon et al, 2010;Han et al, 2014;Hassanin et al, 2015;Singh et al, 2015). The K m values for NAD + were 2.36, 0.08, 0.1, 0.08, 0.18 and 0.042 mM for RDHs from E. agglomerans, G. oxydans, K. aerogenes, R. sphaeroides, Z. mobilis and P. alcalifaciens, respectively (Kahle et al, 1992;Muniruzzaman et al, 1995b;Adachi et al, 2001;Moon et al, 2010;Han et al, 2014;Hassanin et al, 2015).…”