Cloning and characterization of a new ribitol dehydrogenase fromProvidencia alcalifaciensRIMD 1656011

Abstract

The substrate specificity indicated that the ribitol dehydrogenase from P. alcalifaciens RIMD 1656011 can be used for direct production of allitol from d-fructose without any by-product formation. © 2015 Society of Chemical Industry.

Synthesis of allitol from D-psicose using ribitol dehydrogenase and formate dehydrogenase

“…If polyol dehydrogenase is active on these substrates, D-fructose could be reduced to D-sorbitol or D-mannitol, which would result in impurities in the final product [16]. However, the RDH from P. alcalifaciens that we investigated was not active on D-sorbitol and D-mannitol, which indicated that the enzyme can be used for the production of allitol directly from D-fructose [17]. To the best of our knowledge, there is also an RDH from K. pneumonia which is not active on D-sorbitol and D-mannitol [16].…”

“…D-psicose was previously prepared in our laboratory from Dfructose [19]. Two reconstructed plasmids, containing FDH from the methanol-assimilating yeast O. parapolymorpha DL-1 and RDH from P. alcalifaciens RIMD 1656011 were synthesised by Sangon Biological Engineering Technology and Services (Shanghai, China), in accordance with the protocol reported by Hassanin et al [17].…”

“…The use of both bacterial reduction (using Enterobacter strain 221) and enzymatic reaction (using ribitol dehydrogenase: RDH) to produce allitol from Dpsicose has been reported [12,15,16]. In recent studies, a new RDH from Providencia alcalifaciens RIMD 1656011 and formate dehydrogenase (FDH) from the methanolassimilating yeast Ogataea parapolymorpha DL-1 were cloned and characterised [17,18]. The new RDH was found to possess high specificity towards ribitol and allitol and low specificity towards other polyols (D-sorbitol, L-arabitol, galactitol, D-mannitol, inositol, and glycerol) regarding catalytic targeting [17].…”

Allitol: production, properties and applications

“…RDHs have been isolated from various sources, such as Aerobacter aerogenes (Fossitt & Wood, 1966), Enterobacter agglomerans (Muniruzzaman et al, 1995b), Gluconobacter oxydans (Adachi et al, 2001), Klebsiella aerogenes (Taylor et al, 1974), Rhodobacter sphaeroides (Kahle et al, 1992), Klebsiella oxytoca (Han et al, 2104), Z. mobilis (Moon et al, 2010), E. aerogenes (Singh et al, 2015) and Providencia alcalifaciens (Hassanin et al, 2015) (Table 2). In addition, RDH catalyses production of D-ribulose from ribitol.…”

“…The Michaelis-Menten constants (K m ) of RDHs from K. aerogenes, Rhodobacter sphaeroides, E. agglomerans, G. oxydans, Z. mobilis, E. aerogenes and P. alcalifaciens with ribitol were 8, 6.3, 32.2, 1.2, 11.8, 10.3 and 13.9 mM, respectively (Kahle et al, 1992;Muniruzzaman et al, 1995b;Adachi et al, 2001;Moon et al, 2010;Han et al, 2014;Hassanin et al, 2015;Singh et al, 2015). No previous study has examined the kinetic parameters of reduction in D-psicose into allitol by RDH.…”