Production of a new sucrose derivative by transglycosylation of recombinant Sulfolobus shibatae β-glycosidase

Park, Na Young; Baek, Nam In; Cha, Jaeho; Lee, Soo Bok; Auh, Joong-Hyuck; Park, Cheon Seok

doi:10.1016/j.carres.2005.02.003

Cited by 14 publications

(6 citation statements)

References 34 publications

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“…The transglycosylation activity of recombinant b-glycosidase from Sulfolobus shibatae (rSSG) has also been tested using lactose as a donor and various sugar acceptors. 61 With sucrose as acceptor, lactosucrose analogue 56 was produced, which may have similar applications to sucrose as a dietary supplement.…”

Section: Methodsmentioning

confidence: 99%

Recent developments in preparative enzymatic syntheses of carbohydrates

Rowan

Hamilton

2006

Nat. Prod. Rep.

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Section: Methodsmentioning

confidence: 99%

Recent developments in preparative enzymatic syntheses of carbohydrates

Rowan

Hamilton

2006

Nat. Prod. Rep.

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“…Escherichia coli MC1061 strain harboring the cloned DGAS gene (dgas) (Deinococcus geothermalis ASase) in the pHCXHD vector (pHC-dgas) and E. coli BL21(DE3) strain harboring the cloned ssg (Sulfolobus shibatae β-glycosidase) in the pRSET-B vector (pRB-ssg) were utilized for the production of DGAS and SSG, respectively. Purified enzymes were prepared according to the method described in the previous reports [19,25]. In final, DGAS and SSG were then concentrated to 1 and 5 mg/ml, respectively, in the buffer [100 mM sodium citrate buffer (pH 5) and 50 mM Tris-HCl buffer (pH 7) for SSG and DGAS, respectively] using ultrafiltration (30,000 MW cut-off membrane; Amicon, USA).…”

Section: Enzyme Preparationmentioning

confidence: 99%

“…In the presence of appropriate nucleophiles other than water, the biosynthesis of glycoconjugates can occur instead of the hydrolysis reaction [17]. β-Glycosidase driven biosynthesis has been employed in the synthesis of a variety of biologically important compounds [18,19]. In addition, amylosucrase (ASase, E.C.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Synthesis of ��-Glucosylglycerol and Its Unnatural Glycosides Via ��-Glycosidase and Amylosucrase

Jung¹,

Seo²,

Park³

et al. 2019

Journal of Microbiology and Biotechnology

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β-Glucosylglycerol (β-GG) and their derivatives have potential applications in food, cosmetics and the healthcare industry, including antitumor medications. In this study, β-GG and its unnatural glycosides were synthesized through the transglycosylation of two enzymes, Sulfolobus shibatae β-glycosidase (SSG) and Deinococcus geothermalis amylosucrase (DGAS). SSG catalyzed a transglycosylation reaction with glycerol as an acceptor and cellobiose as a donor to produce 56% of β-GGs [β-D-glucopyranosyl-(1 → 1/3)-D-glycerol and β-D-glucopyranosyl-(1 → 2)-D-glycerol]. In the second transglycosylation reaction, β-D-glucopyranosyl-(1 → 1/3)-Dglycerol was used as acceptor molecules of the DGAS reaction. As a result, 61% of α-Dglucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→1/3)-D-glycerol and 28% of α-D-maltopyranosyl-(1→4)-β-D-glucopyranosyl-(1→1/3)-D-glycerol were synthesized as unnatural glucosylglycerols. In conclusion, the combined enzymatic synthesis of the unnatural glycosides of β-GG was established. The synthesis of these unnatural glycosides may provide an opportunity to discover new applications in the biotechnological industry.

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“…β-Glucosidases exist in all living organisms including archaea, bacteria, fungi, plants and animals (Park et al, 2005). These enzymes have been extensively studied due to its strong potential of utilization in the food and medical industries (Briante et al, 2004;Gu et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Construction of recombinant Pichia strain GS115-Ch-Glu expressing β-glucosidase and cyanide hydratase for cyanogenic glycosides detoxification

Wu¹

2012

Afr. J. Biotechnol.

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A recombinant Pichia strain GS115-Ch-Glu expressing both β-glucosidase and cyanide hydratase was constructed to remove cyanogenic glycosides in edible plants. The β-glucosidase could hydrolyze cyanogenic glycosides into cyanide and its gene (Glu) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). A cyanide hydratase could catalyze cyanide to formamide and its gene (Ch) was obtained by C (PCR). Both Glu and Ch genes were integrated into the genome of Pichia strain GS115 by homologous recombination. The engineering strain GS115-Ch-Glu was confirmed by multiple analytical methods, and was inoculated to induce expression of the recombinant Glu and Ch genes. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that the fused proteins of interest had specifically been expressed as 31 and 52 KDa, which corresponded to the predicted molecular weights of the two enzymes. The enzyme preparation containing cyanide hydratase and β-glucosidase produced by strain GS115-Ch-Glu could completely decompose cyanogenic glycosides in flaxseed power into cyanide, and further catalyze the hydrolysis of over 80% cyanide.

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Production of a new sucrose derivative by transglycosylation of recombinant Sulfolobus shibatae β-glycosidase

Cited by 14 publications

References 34 publications

Recent developments in preparative enzymatic syntheses of carbohydrates

Recent developments in preparative enzymatic syntheses of carbohydrates

Enzymatic Synthesis of ��-Glucosylglycerol and Its Unnatural Glycosides Via ��-Glycosidase and Amylosucrase

Construction of recombinant Pichia strain GS115-Ch-Glu expressing β-glucosidase and cyanide hydratase for cyanogenic glycosides detoxification

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