Recent developments in preparative enzymatic syntheses of carbohydrates

Abstract

This review covers advances and developments in the use of enzymes in synthetic mono- and oligosaccharide chemistry. Particular attention is paid to the use of aldolase/ketolases, glycosidases, glycosyltransferases, lipases, and coupled multi-enzyme biotransformations; 172 references are cited

Practical Synthesis of Sugar Monophosphonucleotides

“…The chemical and enzymatic syntheses of many natural, and modified, sugar-NDPs have received considerable attention because of the expanding use of glycosyltransferases as biocatalysts for preparative-scale biotransformations. [3,4] Conversely, the synthesis of hexose-derived sugar-NMPs has been less extensively addressed, and to our knowledge, the potential of native or engineered sialyltransferases as an alternative subset of biotransformation tools, beyond their use with CMP-sialic acid analogues, [5] remains unexplored. To be able to pursue such studies requires routine access to numerous sugar-NMP conjugates.…”

Studying non-covalent enzyme carbohydrate interactions by STD NMR

“…[1][2][3][4][5] They find applications in research and development 1,2,6-8 and are widely used in industrial processes. 9,10 Glycosidases and glycosyltransferases find use in the synthesis of glycosides and oligosaccharides, whereas oxidoreductases, lyases, and ligases are basically applied to modify monosaccharides.…”

“…9,10 Glycosidases and glycosyltransferases find use in the synthesis of glycosides and oligosaccharides, whereas oxidoreductases, lyases, and ligases are basically applied to modify monosaccharides. 1,2,11,12 Such broad range of applications implies a general interest to understand molecular foundations of these diverse catalytic processes. For that purpose, it is necessary to get a detailed knowledge of substrate recognition to understand enzymatic effectiveness and to study roles of enzymes in cellular identification procedures.…”

“…Relative STD effects of glucose (3), cellobiose(1), and lactose (2) bound to AfbGly. (a) Binding pattern of cellobiose (1) at 10°C under binding-only conditions.…”

Enzymatic synthesis of α-glucosyl-timosaponin BII catalyzed by the extremely thermophilic enzyme: Toruzyme 3.0L

“…On the contrary, synthesis of the glycoconjugate by enzymatic transformation is used as an alternative method in manufacturing pharmaceuticals, fine chemicals, and food ingredients, due to the highly selective catalytic reactions, its environmental friendly nature, and mild reaction conditions of biotechnology. [8][9][10][11] It has been found that some enzymes are able to catalyze transglycosylation reactions to synthesize oligosaccharides or glycoconjugate molecules. [12][13][14][15][16] It is known that, Cyclodextrin-glycosyltransferase (CGTase EC 2.4.…”